Korea-Australia Rheology Journal

The Korean Society of Rheology (한국유변학회)
권호 표지

The effect of L-carnosine on the rheological characteristics of erythrocytes incubated in glucose media

  • Received : 2009.03.16
  • Accepted : 2009.04.01
  • Published : 2009.06.30
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Abstract

Hemorheological properties are easily modified by glucose-induced oxidation and glycation. Carnosine, a naturally occurring dipeptide ($\beta$-alanyl-LFull-size image-histidine), has been recently proposed to act as an antioxidant as well as a free-radical scavenger. In the present study, we investigate its protective and rejuvenating effects in erythrocytes that are exposed to glucose-rich plasma. Erythrocytes that were incubated in glucose solutions were treated with different concentrations of carnosine and for different incubation times. Their hemorheological alterations were examined. The results reveal that the presence of carnosine effectively prevented these rheological alterations in a concentration-dependent manner in glucose-rich media. It is proposed that moderate concentrations of carnosine might be further explored as potential therapeutic agents for pathologies that involve hemorheological modification.

Keywords

References

  1. Aydogan, S., H. Yapislar, S. Artis and B. Aydogan, 2008, Impaired erythrocytes deformability in $H_2O_2$-induced oxidative stress: protective effect of L-carnosine, Clin. Hemorheol. Microcir. 39, 93-98
  2. Babu, N. and M. Singh, 2004, Influence of hyperglycemia on aggregation deformability and shape parameters of erythrocytes, Clin. Hemorheol. Microcir. 31, 273-280
  3. Brown, C. D., H. S. Ghali, Z. Zhao, L. L. Thomas and E. A. Friedman, 2005, Association of reduced red blood cell deformability and diabetic nephropathy, Kidney International 67, 295–300 https://doi.org/10.1111/j.1523-1755.2005.00082.x
  4. Crush, K. G., 1970, Carnosine and related substances in animal tissues, Comparative Biochemistry and Physiology 34, 3-30 https://doi.org/10.1016/0010-406X(70)90049-6
  5. Hipkiss, A. R. and C. Brownson, 2000, Review: A possible new role for the anti-ageing peptide carnosine, Cell. Mol. Life Sci. 57, 747–753 https://doi.org/10.1007/s000180050039
  6. Iwata, H., H. Ukeda, T. Maruyama, T. Fujino and M. Sawamura, 2004, Effect of carbonyl compounds on red blood cells deformability, Biochem Biophys Res Commun. 321, 700-706 https://doi.org/10.1016/j.bbrc.2004.07.026
  7. Mullarkey, C. J., D. Edelstein and M. Brownlee, 1990, Free radical generation by early glycation products: a mechanism for accelerated atherogenesis in diabetes, Biochem Biophys Res Commun. 173, 932-939 https://doi.org/10.1016/S0006-291X(05)80875-7
  8. Nagai, K., A. Niijima., T. Yamano, H. Otani, N. Okumra, N. Tsuruoka, M. Nakai and Y. Kiso, 2003, Possible Role of L-Carnosine in the Regulation of Blood Glucose through Controlling Autonomic Nerves, Experimental biology and medicine 228, 1138-1145
  9. Quinn, P. J., A. A. Boldyrev and V. E. Formazuyk, 1992, Carnosine: its properties, functions and potential therapeutic applications, Mol. Aspects Med. 13, 379–444 https://doi.org/10.1016/0098-2997(92)90006-L
  10. Riquelme, B., P. Foresto, M. D’Arrigo, J. Valverde and R. Rasia, 2005, A dynamic and stationary rheological study of erythrocytes incubated in a glucose medium, J. Biochem. Biophys. Methods 62, 131-141 https://doi.org/10.1016/j.jbbm.2004.10.004
  11. Schmid-Schonbein, H. and E. Volger, 1976, Red-cell aggregation and red-cell deformability in diabetes, Diabetes 25 (Suppl 2), 897-902
  12. Seidler, N. W., G. S. Yeargans and T. G. Morgan, 2004, Carnosine disaggregates glycated $\alpha$-crystallin: an in vitro study, Archives of Biochemistry and Biophysics 427, 110-115 https://doi.org/10.1016/j.abb.2004.04.024
  13. Shin, S., Y. Yang and J. S. Suh, 2007, Microchip-based cell aggregometer using stirring-disaggregation mechanism, Korea-Australia Rheology J. 19, 109-115
  14. Shin, S., Y. H. Ku, J. S. Suh and M. Singh, 2008, Rheological characteristics of erythrocytes incubated in glucose media, Clin. Hemorheol. Microcir. 38, 153-161
  15. Shin, S., Y. Yang and J. S. Suh, 2009, Measurement of erythrocyte aggregation in a microchip stirring system by light transmission, Clin. Hemorheol. Microcir. 41, 197–207
  16. Snyder, L. M., N. L. Fortier, J. Trainor, J. Jacobs, L. Leb, B. Lubin, D. Chiu, S. Shohet and N. Mohandas, 1985, Effect of hydrogen peroxide exposure on normal human erythrocyte deformability, morphology, surface characteristics and spectrinhemoglobin cross-linking, J. Clin. Invest. 76, 1971–1977 https://doi.org/10.1172/JCI112196
  17. Tamba, M., and A. Torreggiani, 1999, Hydroxyl radical scavenging by carnosine and Cu (II)-carnosine complexes: a pulseradiolysis and spectroscopic study, Int. J. Radiat. Biol. 75, 1177-1188 https://doi.org/10.1080/095530099139656
  18. Thornalley, P. J., 1998, Cell activation by glycated proteins: AGE receptors, receptor recognition factors and functional classification of AGEs. Cell Mol. Biol. 44, 1013–1023
  19. Yilmaz, F. and M. Gundogdu, 2008, A critical review on blood flow in large arteries; relevance to blood rheology, viscosity models, and physiologic conditions, Korea-Australia Rheology J. 20, 197-211