Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory activity of methanol extract isolated from stem bark of Albizia julibrissin

  • Na, Ho-Jeong (Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Johns Hopkins Asthma Center) ;
  • Cha, Dong-Seok (Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Republic of Korea Department of Medicine) ;
  • Jeon, Hoon (Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Republic of Korea Department of Medicine)
  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Albizia julibrissin (AJ) has been used widely as a traditional medicine. In macrophages nitric oxide (NO) is released as an inflammatory mediator and has been proposed to be an important modulator of many pathophysiological conditions including inflammation and carcinogenesis. In this study we have examined the NO inhibition effect of 85% methanol extracts of AJ in mouse macrophage. Lipopolysaccharide (LPS) has been reported to induce production of NO. Extracts of AJ (1, 10, $100{\mu}g/ml$) suppressed nitric oxide production in LPS-stimulated ($100{\mu}g/ml$) mouse (C57BL/6) macrophages and analyzed by ELISA. In addition, it also attenuated the expression of inflammatory products like Interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), and inducible NOS (iNOS) as assessed by immunoblotting with specific antibodies. These results suggest that 85% methanol extracts of AJ would be useful in inflammatory diseases.

Keywords

References

  1. The Pharmacopoeia Committee of People's Republic of China (ed.), (1995) 'Pharmacopoeia (I),' People Health Press, Beijing, 119-120
  2. Adams DO, Hamilton TA. (1984) The cell biology of macrophage activation. Annu. Rev. Immunol. 2, 283- 318 https://doi.org/10.1146/annurev.iy.02.040184.001435
  3. Ambs S, Merriam WG, Bennett WP, Felley-Bosco E, Ogunfusika MO, Oser SM, Klein S, Shields PG, Billiar TR, Harris CC. (1998) Frequent nitric oxide synthase-2 expression in human colon adenomas: implication for tumor angiogenesis and colon cancer progression. Cancer Res. 58, 334-341
  4. Beutler B. (1995) TNF, immunity and inflammatory disease: lessons of the past decade. J. Invest. Med., 43, 227-235
  5. Beutler B. (2000) Tlr4: central component of the sole mammalian LPS sensor, Curr. Opin. Immuno. 12, 20-26 https://doi.org/10.1016/S0952-7915(99)00046-1
  6. Blackman DJ, Morris-Thurgood JA, Atherton JJ, Ellis G.R, Anderson RA, Cockcroft JR, Frenneaux MP. (2000) Endothelium-derived nitric oxide contributes to the regulation of venous tone in humans. Circulation 18, 165-170
  7. Chung HS, Jeong HJ, Hong SH, Kim MS, Kim SJ, Song BK, Jeong IS, Lee EJ, Ahn JW, Baek SH. Kim HM. (2002) Induction of nitric oxide synthase by Oldenlandia diffusa in mouse peritoneal macrophages. Biol. Pharm. Bull. 25, 1142-1146 https://doi.org/10.1248/bpb.25.1142
  8. Dinarello CA. (2000) Proinflammatory cytokines. Chest 118, 503-508 https://doi.org/10.1378/chest.118.2.503
  9. Goldstein SR, Yang GY, Chen X, Curtis SK, Yang CS. (1998) Studies of iron deposits, inducible nitric oxide synthase and nitrotyrosine in a rat model for esophageal adenocarcinoma. Carcinogenesis 19, 1445-1449 https://doi.org/10.1093/carcin/19.8.1445
  10. Kanazawa K., Kawasaki H, Samejima K, Ashida H, Danno G.. (1995) Specific desmutagen (antimutagen) in oregano against a dietary carcinogen, Trp-P-2, are galangin and quercetin. J. Agric. Food Chem. 43, 404-409 https://doi.org/10.1021/jf00050a028
  11. Kim MS, Lim WK, Cha JG, An NH, Yoo SJ, Park JH, Kim HM, Lee YM. (2001) The activation of PI 3-K and PKC zeta in PMA-induced differentiation of HL-60 cells. Cancer Lett. 171, 79-85 https://doi.org/10.1016/S0304-3835(01)00505-5
  12. Koyanagi M, Egashira K, Kubo-Inoue M, Usui M, Kitamoto S, Tomita H, Shimokawa H, Takeshita A. (2000) Role of transforming growth factor-beta1 in cardiovascular inflammatory changes induced by chronic inhibition of nitric oxide synthesis. Hypertension 35, 86-90 https://doi.org/10.1161/01.HYP.35.1.86
  13. Medzhitov R, Janeway CA Jr. (1997a) Innate immunity: the virtues of a nonclonal system of recognition. Cell 9, 295-298 https://doi.org/10.1016/S0092-8674(00)80412-2
  14. Medzhitov R, Janeway CA. (1997b) Innate immunity: impact on the adaptive immune response., Curr. Opin. Immunol. 9, 4-9 https://doi.org/10.1016/S0952-7915(97)80152-5
  15. Minghetti L, Levi G. (1998) Microglia as effector cells in brain damage and repair: focus on prostanoids and nitric oxide. Prog. Neurobiol. 54, 99-125 https://doi.org/10.1016/S0301-0082(97)00052-X
  16. Mosmann T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  17. Parrillo JE. (1993) Pathogenetic mechanisms of septic shock. New Engl. J. Med. 328, 1471-1477 https://doi.org/10.1056/NEJM199305203282008
  18. Remick DG, Strieter R.M, Eskandari MK, Nguyen DT, Genord MA, Raiford CL, Kunkel SL. (1990) Role of tumor necrosis factor-alpha in lipopolysaccharideinduced pathologic alterations. Am. J. Pathol. 136, 49-60
  19. Sharma R, Coats AJ, Anker SD. (2000) The role of inflammatory mediators in chronic heart failure: cytokines, nitric oxide, and endothelin-1. Int. J. Cardiol. 15, 175-186 https://doi.org/10.1016/S0167-5273(99)00186-2
  20. Thiemermann C, Vane J. (1990) Inhibition of nitric oxide synthesis reduces the hypotension induced by bacterial lipopolysaccharides in the rat in vivo. Eur. J. Pharmacol. 182, 591-595 https://doi.org/10.1016/0014-2999(90)90062-B
  21. Vilcek J, Lee TH. (1991) Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions. J. Biol. Chem. 266, 7313-7316
  22. Wallace JL. (1999) Distribution and expression of cyclooxygenase (COX) isoenzymes, their physiological roles, and the categorization of nonsteroidal antiinflammatory drugs (NSAIDs). Am. J. Med., 13, 107, 11S-16S; discussion 16S-17S https://doi.org/10.1016/S0002-9343(99)00363-0
  23. Wilson KT, Fu S, Ramanujam KS, Meltzer SJ. (1998) Increased expression of inducible nitric oxide synthase and cyclooxygenase-2 in Barrett's esophagus and associated adenocarcinomas. Cancer Res., 58, 2929-2934
  24. Zhang RY. (1999) Pharmacopoeia Committee of China. Chinese Pharmacopoeia, C-hemical Industry Press: Beijing, 2005; Vol. 1, p 97. S. Q.; Chem. J. Chin. Univ. 20, 1877