Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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In vitro anti oxidant activity of methanol extract of Clerodendrum infortunatum Linn

  • Sannigrahi, Santanu (Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences) ;
  • Mazumder, Upal Kanti (Department of Pharmaceutical Technology, Jadavpur University) ;
  • Pal, Dilip Kumar (Department of Pharmaceutical Chemistry, Seemanta Institute of Pharmaceutical Sciences) ;
  • Parida, Sambit (Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences)
  • Published : 2009.06.30
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Abstract

The antioxidant potency of methanolic extract Clerodendrum infortunatum Linn. (MECI), which are widely used in the Indian indigenous system of medicine for different purposes, was studied. The antioxidant potential was evaluated using different established in vitro antioxidant tests viz. determination of total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, nitric oxide scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging and reductive power assay. It was found that MECI contain a high amount of polyphenolics and possesses significant free radical scavenging activity in all the assay. The higher activity was may be due to presence of richest amount of polyphenolics and flavonoids in it.

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References

  1. Akihisa T, Matsubara Y, Ghosp P, Thakur S, Tamura T, Matsumoto T. (1989) Sterols of some Clerodendrum species (Verbanaceae): Occurrence of the 24-$\alpha$- and 24-$\beta$- epimer of 24 ethylsterols lacking a $\delta$-25-bond. Steroids 55, 625-638 https://doi.org/10.1016/0039-128X(89)90036-6
  2. Aruoma OI. (1998) Free radicals, oxidative stress, and antioxidants in human health and diseases. J. Am. Oil Chem. Soc. 75, 199-212 https://doi.org/10.1007/s11746-998-0032-9
  3. Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F, Tripathi PC, Seal T, Mukherjee B. (2003) Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. J. Ethnopharmacol. 84, 131-138 https://doi.org/10.1016/S0378-8741(02)00322-7
  4. Blois MS. (1968) Antioxidant determination by the use of a stable free radical. Nature 29, 1199-1200
  5. Branen AL. (1975) Toxicology and biochemistry of butylated hydroxyanisol and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 5, 59-63 https://doi.org/10.1007/BF02901825
  6. Chae S, Kim JS, Kang KA, Bu HD, Lee Y, Hyun JW, Kang SS. (2004) Antioxidant activity of Jionoside D from Clerodendron trichotomum. Biol. Pharm. Bull. 27, 1504-1508 https://doi.org/10.1248/bpb.27.1504
  7. Chae S, Kim JS, Kang KA, Bu HD, Lee Y, Seo YR, Hyun JW, Kang SS. (2005) Antioxidant activity of isoacteoside from Clerodendron trichotomum. J. Toxicol. Environ. Health A. 68, 389-400 https://doi.org/10.1080/15287390590900750
  8. Chopra RN, Nayer SL, Chopra IC. (1992) Glossay of Indian medicinal plant. pp. 71, Publication and Information Directorate, CSIR, New Delhi
  9. Clavin M, Gorzalczany S, Macho A, Munoz E, Ferraro G, Acevedo C, Martino V. (2007) Anti-inflammatory activity of flavonoids from Eupatorium arnottianum. J. Ethnopharmacol. 12, 585-589 https://doi.org/10.1016/j.jep.2007.04.007
  10. Cook NC, Samman S. (1996) Flavonoids- chemistry, metabolism, cardio-protective effects, and dietary sources. Nutritional effects and dietary sources. Nutritional Biochem. 7, 66- 76 https://doi.org/10.1016/0955-2863(95)00168-9
  11. Elizabeth K, Rao MNA. (1990) Oxygen radical scavenging activity of curcumin. Int. J. Pharma. 58, 237-240 https://doi.org/10.1016/0378-5173(90)90201-E
  12. Frautchy SA, Hu W, Kim P, Miller SA, Chu T, Harris-White ME, Cole GM. (2001) Phenolic anti-inflammatory antioxidant reversal of Abeta-induced cognitive deficits and neuropathology. Neurobiol. Aging 22, 993-1005 https://doi.org/10.1016/S0197-4580(01)00300-1
  13. Gopal N, Sengottuvelu S. (2008) Hepatoprotective activity of Clerodendrum inerme against $CCl_4$ induced hepatic injury in rats. Fitoterapia 79, 24-26 https://doi.org/10.1016/j.fitote.2007.07.006
  14. Gordon MH. (1990) The mechanism of the antioxidant action in vitro. In Food Antioxidants, edited by Hudson BJF, pp. 1-18, Elsevier, London
  15. Goswami A, Dixit VK, Srivastava BK. (1998) Antimalarial activity of aqueous extract of Clerodendrum infortunatum. Bionature 48, 45-48
  16. Grice HP. (1988) Enhanced tumour development by butylated hydroxyanisole (BHA) from the prospective of effect on fore-stomach and oesophageal squamous epithelium. Food Chem. Toxicol. 26, 717-723 https://doi.org/10.1016/0278-6915(88)90072-5
  17. Gutteridgde JMC. (1995) Free radicals in disease processes: A complication of cause and consequence. Free Rad. Res. Comm. 19, 141-158 https://doi.org/10.3109/10715769309111598
  18. Halliwell B, Gutteridge JMC, Aruoma OI. (1987) The deoxyribose method: a simple 'test-tube' assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem. 165, 215-219 https://doi.org/10.1016/0003-2697(87)90222-3
  19. Halliwell B. (2000) The antioxidant paradox. Lancet 355, 1179-1180 https://doi.org/10.1016/S0140-6736(00)02075-4
  20. Joshi KC, Prakash L, Shah RK. (1978) Chemical constituents of Clerodendrum infortunatum and Ficus racemosa. J. Indian Chem. Soc. 54, 1104
  21. Khandelwal KR. (2000) Practical Pharmacognosy Techniques and Experiments, 9th edition, Nirali Prkashan, Pune, India
  22. Kirtikar KR, Basu BD. (2001) Indian Medicinal Plants Vol. VIII, p-2686 editors Mhaskar KS, Blatter E and Caius JF, Sri Satguru Publications, Delhi
  23. Koleva II, Van Beek TA, Linssen JPH, de Groot A, Evstatieva LN. (2002) Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal. 13, 8-17 https://doi.org/10.1002/pca.611
  24. Mantle D, Eddeb F, Pickering AT. (2000) Comparison of relative antioxidant activities of British medicinal plant species in vitro. J. Ethnopharmacol. 72, 47-51 https://doi.org/10.1016/S0378-8741(00)00199-9
  25. Marcocci L, Maguire JJ, Droy-Lafaix MT, Packer L. (1994) The nitric oxide scavenging property of Ginkgo biloba extracts EGb 761. Biochem. Biophys. Res. Commun. 201, 748-755 https://doi.org/10.1006/bbrc.1994.1764
  26. Miller MJ, Sadowska-Krowicka H, Chotinaruemol S, Kakkis JL, Clark DA. (1993) Amelioration of chronic ileitis by nitric oxide synthase inhibition. J. Pharmacol. Exp. Ther. 264, 11-16
  27. Moller JKS, Madsen HL, Altonen T, Skibsted LH. (1999) Dittany (Origanum dictamnus) as asource of waterextractable antioxidants. Food Chem. 64, 215–219 https://doi.org/10.1016/S0308-8146(98)00143-5
  28. Nadkarni KM, Nadkarni AK. (2002) Indian Materia Medica, p. 353, Popular Publications, Mumbai
  29. Nishimiki M, Rao NA, Yagi K. (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem. Biophys. Res. Comm. 46, 849-853 https://doi.org/10.1016/S0006-291X(72)80218-3
  30. Oyaizu M. (1986) Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nut. 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  31. Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Saavedra G, Murcia MA, Jimenez AM, Codina C. (2003) Investigation of bovilian plant extracts for their radical scavenging activity and antioxidant activity. Life Sci. 73, 1667-1681 https://doi.org/10.1016/S0024-3205(03)00488-0
  32. Pin-Der Duh. (1998) Antioxidant activity of Budrock (Arctium lappa Linn): Its scavenging effect on free radical and active oxygen. J. American Oil Chem. Soc. 75, 455-461 https://doi.org/10.1007/s11746-998-0248-8
  33. Rajakaruna N, Harris CS, Towers GHN. (2002) Antimicrobial Activity of Plants Collected from Serpentine Outcrops in Sri Lanka. Pharm. Biol. 40, 235-244 https://doi.org/10.1076/phbi.40.3.235.5825
  34. Rajlakshmi D, Banerjee SK, Sood S, Maulik SK. (2003) In-vitro and in-vivo antioxidant activity of different extracts of the leaves of Clerodendron colebrookianum Walp in the rat. J. Pharm. Pharmacol. 55, 1681-1686 https://doi.org/10.1211/0022357022296
  35. Schuler P. (1990) Natural antioxidants exploited commercially. In: Food Antioxidants edited by Hudson BJF, pp. 99-170, Elsevier, London
  36. Sinha NK, Seth KK, Pandey VB, Dasgupta B, Shah A. (1981) Flavonoids from the flower of Clerodendrum infortunatum. Planta Med. 42, 296-298 https://doi.org/10.1055/s-2007-971645
  37. Slinkard K, Singleton VL. (1977) Total phenol analyses: automation and comparison with manual methods. Am. J. Enol. Vitic. 28, 49-55
  38. Wang L, Tu YC, Lian TW, Hung JT, Yen JH, Wu MJ. (2006) Distinctive antioxidant and anti inflammatory effects of flavonols. J. Agric. Food Chem. 54, 9798-9804 https://doi.org/10.1021/jf0620719
  39. Wichi HC. (1986) Safety evaluation of butylated hydroxytoluene (BHT) in the liver, lung and gastrointestinal tract. Food Chem. Toxicol. 24, 1127-1130 https://doi.org/10.1016/0278-6915(86)90298-X

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