Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activity and Antioxidant Components in Methanolic Extract from Geumjong Rice

금종쌀 Methanol 추출물의 항산화 성분 및 항산화 효과

  • Kong, Su-Hyun (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Choi, Young-Min (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Young-Wha (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Dae-Jung (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
  • Published : 2009.06.30
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Abstract

In this study, the antioxidant compounds and antioxidant activities of Geumjong brown rice were measured to evaluate functional properties and to compare with white rice and Chuchung brown rice. The content of polyphenolics, flavonoids and $\gamma$-oryzanol were measured by spectrophometric methods and tocopherol and tocotrienol analyses were carried out by HPLC. ABTS and DPPH radical scavenging activity and reducing power were used to measure antioxidant activity. The extraction yield of Chuchung white rice, Chuchung brown rice, and Geumjong brown rice were 1.5, 2.3, and 3.5%, respectively. The high level of antioxidant activity (69.77 mg TEAC/100 g) and total polyphenolic content (69.1 mg gallic acid equivalents/100 g) were found in Geumjong brown rice, whereas the reducing power of Geumjong brown rice was lower than that of Chuchung brown rice.

본 연구에서는 도정되지 않은 현미상태의 금종쌀(Geumjong brown rice) methanol 추출물의 항산화 성분과 항산화활성을 측정하여 새로운 품종의 기능적 가치를 평가하고자 하였다. 이와 함께 백미(Chuchung white rice) 및 현미(Chuchung brown rice)와의 항산화 성분과 항산화력을 비교 분석하고자 하였다. 항산화 성분으로는 polyphenol, flavonoid, vitamin E와 $\gamma$-oryzanol을 측정하였으며 항산화력은 ABTS와 DPPH 라디칼 제거능 및 환원력을 측정하였다. 백미 및 현미, 금종쌀은 각각 1.5, 2.3, 그리고 3.5%의 추출수율을 나타냈다. 실험결과, 금종쌀은 높은 항산화 활성(69.77 mg TEAC/100 g)과 polyphenol함량(69.1 mg gallic acid equivalents/100 g)을 나타냈다. 반면에 환원력에 있어서는 현미(Chuchung brown rice)보다 낮게 측정되었다.

Keywords

References

  1. Anderson JW. 2003. Whole grains protect against atherosclerotic cardiovascular disease. Proc Nutr Soc 62: 135-142 https://doi.org/10.1079/PNS2002222
  2. Chatenoud L, Tavani A, Vecchia CL, Jacobs DR Jr, Negri FL, Franceschi S. 1998. Whole grain food intake and cancer risk. Int J Cancer 77: 24-28 https://doi.org/10.1002/(SICI)1097-0215(19980703)77:1<24::AID-IJC5>3.0.CO;2-1
  3. Kahlon TS, Saunders RM, Sayre RN, Chow FI, Chiu MM, Betschart AA. 1992. Cholesterol-lowering effects of rice bran and rice bran oil fractions in hypercholesterolemic hamsters. Cereal Chem 69: 485-489
  4. Nam SH, Choi SP, Kang MY. 2005. Antioxidative, antimutagenic, and anticarcinogenic ativities of rice bran extracts in chemical and cell assays. J Agric Food Chem 53: 816-822 https://doi.org/10.1021/jf0490293
  5. Chotimarkorn C, Benjakul S, Silalai N. 2008. Antioxidant components and properties of five long grained rice bran extracts from commercial available cultivars in Thailend. Food Chem 111: 636-641 https://doi.org/10.1016/j.foodchem.2008.04.031
  6. Slavin JL, Martini MC, Jacobs DR, Marqart L. 1999. Plausible mechanisms for the protectiveness of whole grains. Am J Clin Nutr 70: 459S-463S
  7. Choi Y, Jeong HS, Lee J. 2007. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 103: 130-138 https://doi.org/10.1016/j.foodchem.2006.08.004
  8. Hu C, Zawistowski J, Ling W, Kitts DD. 2003. Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J Agric Food Chem 51: 5271-5277 https://doi.org/10.1021/jf034466n
  9. Velioglu YS, Mazza G, Cao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruit, vegetables, and grain products. J Agric Food Chem 46: 4113-4117 https://doi.org/10.1021/jf9801973
  10. Jia Z, Tang M, Wu J. 1999. The determination of flavonoid contents in mulberry and they scavenging effects on superoxide radicals. Food Chem 64: 555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  11. Lee SM, Lee J. 2006. Tocopherol and tocotrienol contents of vegetable oils, margarines, butters, and peanut butters consumed in korean diet. Food Sci Biotechnol 15: 183-188
  12. Lilitchan S, Tangprawat C, Aryusuk K, Krisnangkura S, Chokmoh S, Krisnangkura K. 2008. Partial extraction method for the rapid analysis of total lipids and $\gamma$-oryzanol contents in rice bran. Food Chem 106: 752-759 https://doi.org/10.1016/j.foodchem.2007.06.052
  13. Kim DO, Lee KW, Lee HJ, Lee CY. 2002. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J Agric Food Chem 50: 3713-3717 https://doi.org/10.1021/jf020071c
  14. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  15. Mau JL, Lin HC, Song SF. 2002. Antioxidant properties of several specialty mushrooms. Food Res Int 35: 519-526 https://doi.org/10.1016/S0963-9969(01)00150-8
  16. Zielinski H, Kozlowska H. 2000. Antioxidant activity and total phenolics in selected grains and their different morphological fractions. J Agric Food Chem 48: 2008-2016 https://doi.org/10.1021/jf990619o
  17. Seo SJ, Choi YC, Lee SM, Kong S, Lee J. 2007. Antioxidant activities and antioxidant compounds of some specialty rices. J Korean Soc Food Sci Nutr 37: 129-135 https://doi.org/10.3746/jkfn.2008.37.2.129
  18. Zhou Z, Robards K, Helliwell S, Blanchard C. 2004. The distribution of phenolic acids in rice. Food Chem 87: 401- 406 https://doi.org/10.1016/j.foodchem.2003.12.015
  19. Zhimin X, Samuel GJ. 1999. Purification and identification of components of $\gamma$-oryzanol in rice bran oil. J Agric Food Chem 47: 2724-2728 https://doi.org/10.1021/jf981175j
  20. Seo SJ, Choi YC, Lee SM, Kim KJ, Son JR, Lee J. 2007. Determination of selected antioxidant compounds in specialty rice. J Korean Soc Food Sci Nutr 36: 499-502 https://doi.org/10.3746/jkfn.2007.36.4.499
  21. Lee SM, Lee HB, Lee J. 2006. Comparison of extraction methods for the determination of vitamin E in some grains. J Korean Soc Food Sci Nutr 35: 248-253 https://doi.org/10.3746/jkfn.2006.35.2.248
  22. Shimade K, Fujikawa K, Yahara K, Nakamura T. 1992. Antioxidant properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 40: 945-948 https://doi.org/10.1021/jf00018a005

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