Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Antioxidant Activity of Seaweed Extracts from Kongsfjorden, an Inlet in the Arctic's Svalbard Archipelago

북극 스발바드 군도 콩스피요르드에서 채집한 해조류 추출물의 항산화 활성

  • Lee, Jung-Im (Division of Marine Environment & Bioscience Korea Maritime University) ;
  • Kim, You-Ah (Division of Marine Environment & Bioscience Korea Maritime University) ;
  • Kong, Chang-Suk (Marine Bioprocess Research Center Pukyong National University) ;
  • Yea, Sung-Su (Department of Biochemistry, College of Medicine Inje University) ;
  • Han, Tae-Jun (Department of Biology University of Incheon) ;
  • Kang, Sung-Ho (Korea Polar Research Institute, KORDI) ;
  • Kim, Jee-Hee (Korea Polar Research Institute, KORDI) ;
  • Seo, Young-Wan (Division of Marine Environment & Bioscience Korea Maritime University)
  • 이정임 (한국해양대학교 해양환경.생명과학부) ;
  • 김유아 (한국해양대학교 해양환경.생명과학부) ;
  • 공창숙 (부경대학교 해양바이오세스연구단) ;
  • 예성수 (인제대학교 의과대학 생화학교실) ;
  • 한태준 (인천대학교 생물학과) ;
  • 강성호 (한국해양연구원 부설 극지연구소) ;
  • 김지희 (한국해양연구원 부설 극지연구소) ;
  • 서영완 (한국해양대학교 해양환경.생명과학부)
  • Published : 2009.06.30
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Abstract

We examined the intracellular antioxidative effects of 20 Arctic seaweed extracts in Raw 264.7 cells. Each seaweed species was subjected to extraction using acetone/dichloromethane and methanol, respectively, after which the extracts were combined and used as the test sample. The antioxidant ability of all 20 seaweeds extracts was evaluated using four different activity tests, including the degree of occurrence of intracellular reactive oxygen species (ROS), $ONOO^-$, and lipid peroxidation in Raw 264.7 cells, as well as the extent of oxidative damage of genomic DNA purified from Raw 264.7 cells. Crude extracts from Monostroma obscurum, Alaria esculnta, Laminaria digitata, Desmarestia aculeata, Chorda filum, Ptilota seriata, Phycidrys rubens, Devaleraea ramentacea and Palmaria palmata exhibited significant scavenging effects on the generation of intracellular ROS. Among them, Monostroma obscurum and Phycidrys rubens significantly inhibited membrane lipid peroxidation and DNA oxidation. Moreover, Phycidrys rubens exhibited scavenging effects on peroxynitrite generated from SIN-1.

Keywords

References

  1. Azuma K, Nakayama M, Koshika M, Lpoushi K, Yamaguchi Y, Kohata K, Yamaguchi Y, Ito H, Higashio H (1999) Phenolic antioxidants from the leaves of Corchorus olitorius L. J Agric Food Chem 47:3963-3966 https://doi.org/10.1021/jf990347p
  2. Dawson TM, Snyder SH (1994) Gases as biological messenger: nitric oxide and carbon monoxide in the brain. J Neurosci 14:5147-5159
  3. Diplock AT, Charleus JL, Crozier-willi G, Kok FJ, Rice-Evans C, Roberfroid, M., Stahl, W., Vina-Ribes, J. (1998) Functional food science and defence against reactive oxidative species. Brit J Nutr 80:S77-S112 https://doi.org/10.1079/BJN19980106
  4. Frankel EN, Waterhous AI, Kinsella JE (1993) Inhibition of human LDL oxidation by reveratrol. Lancet 341:1103-1104 https://doi.org/10.1016/0140-6736(93)92472-6
  5. Giese J (1996) Antioxidants tolls for preventing lipid foods and their on food quality. Food Chem 57:51-55 https://doi.org/10.1016/0308-8146(96)00067-2
  6. Ha TY, Kang SH, Kwon TY, Ahn JY, Kim SR, Kim DS (2006) Antioxidant activity and contents of bioactive components in Polar Microalgae. Ocean and Polar Res. 28:37-43 https://doi.org/10.4217/OPR.2006.28.1.037
  7. Halliwell B (1996) Antioxidants in human health and disease. Annu Rev Nutr 16:33-50 https://doi.org/10.1146/annurev.nu.16.070196.000341
  8. Hansen MB, Nielsen SE, Berg K (1989) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Meth 119:203-210 https://doi.org/10.1016/0022-1759(89)90397-9
  9. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189-198 https://doi.org/10.1016/0003-9861(68)90654-1
  10. Ichiniose M (2003) Inflammatory mechanisms in bronchial asthma and COPD. Tohuku J Exper Med 200:1-6 https://doi.org/10.1620/tjem.200.1
  11. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS (1994) Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med 16:149-156 https://doi.org/10.1016/0891-5849(94)90138-4
  12. Milne L, Nicotera P, Orrenius S, Burkitt M (1993) Effects of glutathione and chelating agents on copper-mediated DNA oxidation: pro-oxidant and antioxidant propertise of glutathione. Arch Biochem Biophys 304:102-109 https://doi.org/10.1006/abbi.1993.1327
  13. Okimoto Y, Watanabe A, Niki E, Yamashita T, Noguchi N (2000) A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett 474:137-140 https://doi.org/10.1016/S0014-5793(00)01587-8
  14. Regoli F, Nigro M, Bompadre S, Winston G (2000) Total oxidant scavenging capacity (TOSC) of microsomal and cytosolic fractions from Antarctic, Arctic and Mediterranean scallops : differentiation between three potent oxidants. Aquat Toxicol 49:13-25 https://doi.org/10.1016/S0166-445X(99)00070-3
  15. Rice-Evans CA, Miller HJ, Oaganga G (1996) Structure antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol Med 20:933-956 https://doi.org/10.1016/0891-5849(95)02227-9
  16. Rein D, Paglieroni TG, Wun T, Oearson DA, Schmitz HH, Gosselin R, Keen CL (2000) Cocoa inhibits platelet activation and function. Am J Clin Nutr 72:30-35
  17. Seo Y, Lee HJ, Kim YA, Lee JI, Kim JH, Chung H, Kang SH (2007) In vitro Peroxynitrite Scavenging Activity of Arctic Seaweed Extracts. Ocean and Polar Res 29:187-191 https://doi.org/10.4217/OPR.2007.29.2.187

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