Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Isolation and Characteristics of Brachybacterium sp. SY -97 Killing the Harmful Dinoflagellate Cochlodinium polykrikoides

유해적조생물 Cochlodinum polykrikoides를 살멸하는 Brachybacterium sp. SY-97의 분리 및 특성

  • Kim, Yun-Sook (Department of Microbiology, Pukyong National University) ;
  • Jeong, Seong-Yun (International Ginseng & Medical Plant Research Institute) ;
  • Lee, Sang-Joon (Department of Microbiology, Pusan National University) ;
  • Lee, Won-Jae (Department of Microbiology, Pukyong National University)
  • Published : 2009.04.30
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Abstract

A bacterial strain SY-97 that showed algicidal activity against Cochlodinium polykrikoides was isolated from coastal water of Uljin (eastern coast of Korea) in August, 2005. The isolated strain was identified as Brachybacterium sp. by morphological and biological tests, and analysis of 16S rDNA sequence. The optimal culture conditions for the growth of strain SY-97 were $30^{\circ}C$, initial pH 7.0, and salinity 2.0%. From the result of cell culture insert experiment, Brachybacterium sp. SY-97 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.2{\times}10^4\;cells/m{\ell}$) cultures, 100% of C. polykrikoides cells was destroyed within 15 hours. The released algicides were heat-tolerant to $100^{\circ}C$ and stable in pH $6.0{\sim}10.0$. These results suggest that Brachybacterium sp. SY-97 is potentially useful for controlling outbreaks of C. polykrikoides.

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