Journal of Microbiology and Biotechnology

  • 제19권4호
  • /
  • Pages.409-415
  • /
  • 2009
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Degradation and Detoxification of Disperse Dye Scarlet RR by Galactomyces geotrichum MTCC 1360

  • Jadhav, S.U. (Department of Microbiology, Shivaji University) ;
  • Ghodake, G.S. (Department of Biochemistry, Shivaji University) ;
  • Telke, A.A. (Department of Biochemistry, Shivaji University) ;
  • Tamboli, D.P. (Department of Biotechnology, Shivaji University) ;
  • Govindwar, S.P. (Department of Biochemistry, Shivaji University)
  • 발행 : 2009.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Galactomyces geotrichum MTCC 1360 degraded the Scarlet RR(100 mg/l) dye within 18 h, under shaking conditions(150 rpm) in malt yeast medium. The optimum pH and the temperature for decolorization were pH 12 and $50^{\circ}C$, respectively. Enzymatic studies revealed an induction of the enzymes, including flavin reductase during the initial stage and lignin peroxidase after complete decolorization of the dye. Decolorization of the dye was induced by the addition of $CaCO_3$ to the medium. EDTA had an inhibitory effect on the dye decolorization along with the laccase activity. The metabolites formed after complete decolorization were analyzed by UV-VIS, HPLC, and FTIR. The GC/MS identification of 3 H quinazolin-4-one, 2-ethylamino-acetamide, 1-chloro-4-nitro-benzene, N-(4-chloro-phenyl)-hydroxylamine, and 4-chloro-pheny-lamine as the final metabolites corroborated with the degradation of Scarlet RR. The phytotoxicity study revealed the nontoxic nature of the final metabolites. A possible degradation pathway is suggested to understand the mechanism used by G. geotrichum and thereby aiding development of technologies for the application of this organism to the cleaning-up of aquatic and terrestrial environments.

키워드

참고문헌

  1. Abadulla, E., T. Tzanov, S. Costa, K. H Robra, A. Cavaco-Paulo, and G. M. Gubitz. 2000. Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl. Environ. Microbiol. 66: 3357-3362 https://doi.org/10.1128/AEM.66.8.3357-3362.2000
  2. Eichlerova, I., L. Homolka, and F. Nerud. 2006. Evaluation of synthetic dye decolorization capacity in Ischnoderma resinosum. J. Ind. Microbiol. Biotechnol. 33: 759-766 https://doi.org/10.1007/s10295-006-0102-7
  3. Flurkey, W. H., B. Ratcliff, L. Lopez, J. Kunglin, and R. M. Dawley. 1995. Differentiation of fungal tyrosinases and laccase using selective inhibitors and substrates, pp. 805-814. In C. Y. Lee and J. R. Whitaker (eds.), Enzymatic Browning and its Prevention. American Chemical Society, Washington DC
  4. Govindwar, S. and R. Dalvi. 1991. Stimulatory and inhibitory effect of dimethyl sulfoxide on microsomal aniline hydroxylase activity. Toxicol. Lett. 55: 317-323 https://doi.org/10.1016/0378-4274(91)90013-V
  5. Jadhav, S. U., S. D. Kalme, and S. P. Govindwar. 2007. Biodegradation of methyl red by Galactomyces geotrichum MTCC 1360. Inter. Biodeter. Biodegrad. (doi: 10.1016/ j.ibiod.2007.12.010)
  6. Kalme, S. D., G. K Parshetti, S. U. Jadhav, and S. P. Govindwar. 2007. Biodegradation of benzidine based dye direct Blue-6 by Pseudomonas desmolyticum NCIM 2112. Bioresour. Technol. 98: 1405-1410 https://doi.org/10.1016/j.biortech.2006.05.023
  7. Kandelbauer, A., A. Erlacher, A. Cavaco-Paulo, and G. Guebitz. 2004. Laccase-catalyzed decolorization of the synthetic azo dye Diamond Black PV 200 and of some structurally related derivatives. Biocat. Biotrans. 22: 331-339 https://doi.org/10.1080/10242420400024573
  8. Khehra, M. S., H. S. Saini, D. K. Sharma, B. S. Chadha, and S. S. Chimni. 2005. Comparative studies on potential of consortium and constituent pure bacterial isolates to decolorize azo dye. Water Res. 39: 5135-5141 https://doi.org/10.1016/j.watres.2005.09.033
  9. Macedo, J. M. B., L. M. F. Gottschalk, and E. P. S. Bon. 1999. Calcium carbonate mediates higher lignin peroxidase activity in the culture supernatant of Streptomyces viridosporus T7A. Braz. J. Chem. Eng. [online] 16: 163-169 https://doi.org/10.1590/S0104-66321999000200008
  10. Mali, P. L., M. M. Mahajan, D. P. Patil, and M. V. Kulkarni. 1999. Biodecolorizations of membranes of triphenylmethane and azo groups of dyes. J. Sci. Ind. Res. 59: 221-224
  11. Moosvi, S., H. Keharia, and D. Madamwar. 2005. Decolourization of textile dye reactive violet 5 by a newly isolated bacterial consortium RVM 11.1. World J. Microbiol. Biotechnol. 21: 667-672 https://doi.org/10.1007/s11274-004-3612-3
  12. Nagai, M., T. Sato, H. Watanabe, K. Saito, M. Kawata, and H. Enei. 2002. Purification and characterization of an extracellular laccase from the edible mushroom Lentinula edodes, and decolorization of chemically different dyes. Appl. Microbiol. Biotechnol. 60: 327-335 https://doi.org/10.1007/s00253-002-1109-2
  13. Niku-Paavola, M. L., R. Fagerström, K. Kruus, and L. Viikari. 2004. Thermostable laccases produced by a white-rot fungus from Peniophora species. Enz. Microbiol. Technol. 35: 100-102 https://doi.org/10.1016/j.enzmictec.2004.03.008
  14. Pajot, H. F., L. I. C. deFigueroa, and J. I. Farina. 2007. Dyedecolorizing activity in isolated yeasts from the ecoregion of Las Yungas (Tucum'an, Argentina). Enz. Microbiol. Technol. 40: 1503-1511 https://doi.org/10.1016/j.enzmictec.2006.10.038
  15. Parshetti, G., S. Kalme, G. Saratale, and S. Govindwar. 2006. Biodegradation of malachite green by Kocuria rosea MTCC 1532. Acta Chim. Slov. 53: 492-498
  16. Pourbabaee, A. A., F. Malekzadeh, M. N. Sarbolouki, and F. Najafi. 2006. Aerobic decolorization and detoxification of a disperse dye in textile effluent by a new isolate of Bacillus sp. Biotechnol. Bioeng. 93: 631-635 https://doi.org/10.1002/bit.20732
  17. Russ, R., J. Rau, and A. Stolz. 2000. The function of cytoplasm flavin reductases in the reduction of azo dyes by bacteria. Appl. Environ. Microbiol. 66: 1429-1434 https://doi.org/10.1128/AEM.66.4.1429-1434.2000
  18. Salokhe, M. D. and S. P. Govindwar. 1999. Effect of carbon source on the biotransformation enzymes in Serratia marcescens. World J. Microbiol. Biotechnol. 15: 229-232
  19. Sani, R. and U. Banerjee. 2003. Decolorization of triphenylmethane dyes and textile and dye stuff effluent by Kuruthia sp. Enz. Microbiol. Technol. 24: 433-437 https://doi.org/10.1016/S0141-0229(98)00159-8
  20. Saratale, G. D., S. D. Kalme, and S. P. Govindwar. 2006. Decolourization of textile dyes by Aspergillus ochraceus. Ind. J. Biotechnol. 5: 407-410
  21. Shanmugam, V., M. Kumari, and K. D. Yadav. 1999. n-Propanol as a substrate for assaying the lignin peroxidase activity of Phanerochaete chrysosporium. Ind. J. Biochem. Biophys. 36: 39-43
  22. Shrivastava, R., V. Christian, and B. R. M. Vyas. 2005. Enzymatic decolorization of sulfonpthalein dyes. Enz. Microbiol. Technol. 36: 333-337 https://doi.org/10.1016/j.enzmictec.2004.09.004
  23. Wong, P. and P. Yuen. 1998. Decolorization and biodegradation of N,N-dimethyl-p-phenylenediamine by Klebsiella pneumoniae RS-13 and Acinitobacter liquifacienss-1. J. Appl. Microbiol. 85: 79-87 https://doi.org/10.1046/j.1365-2672.1998.00479.x
  24. Xiao, Y. Z., X. M. Tu, J. Wang, M. Zhang, Q. Cheng, W. Y. Zeng, and Y. Y. Shi. 2003. Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp. strain AH28-2. Appl. Microbiol. Biotechnol. 60: 700-707 https://doi.org/10.1007/s00253-002-1169-3
  25. Xueheng, Z. and I. R. Hardin. 2007. HPLC and spectrophotometric analysis of biodegradation of azo dyes by Pleurotus ostreatus. Dyes Pigments 73: 322-325 https://doi.org/10.1016/j.dyepig.2005.11.014

피인용 문헌

  1. Exploring the potential of natural bacterial consortium to degrade mixture of dyes and textile effluent vol.64, pp.7, 2009, https://doi.org/10.1016/j.ibiod.2010.07.001
  2. Biochemical characterization of laccase from hairy root culture of Brassica juncea L. and role of redox mediators to enhance its potential for the decolorization of textile dyes vol.234, pp.6, 2011, https://doi.org/10.1007/s00425-011-1469-x
  3. Decolorization of aqueous solutions of disperse textile dyes by oxidoreductases vol.30, pp.1, 2009, https://doi.org/10.3109/10242422.2012.645339
  4. Microbial biotechnology for decolorization of textile wastewaters vol.14, pp.1, 2009, https://doi.org/10.1007/s11157-014-9344-4
  5. Degradation and detoxification of Navy Blue CBF dye by native bacterial communities: an environmental bioremedial approach vol.57, pp.50, 2009, https://doi.org/10.1080/19443994.2016.1138145
  6. Descoloração do corante Azul Brilhante de Remazol R por leveduras isoladas de moluscos do Rio Subaé, no estado da Bahia, Brasil vol.22, pp.6, 2009, https://doi.org/10.1590/s1413-41522017157568
  7. Biodegradation and Detoxification Efficiency of Azo-Dye Reactive Orange 16 by Pichia kudriavzevii CR-Y103 vol.229, pp.1, 2009, https://doi.org/10.1007/s11270-017-3668-y