Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of High Protein Diet and Resveratrol Supplementation on the Nutritional Status and Immunoreactivity in the Irradiation-induced Inflammatory Rats

방사선 조사된 흰쥐에서 고단백식이와 레스베라트롤 첨가가 영양상태 및 면역기능 증진에 미치는 효과

  • Kim, Kyoung-Ok (Division of Biological Science, College of Science, Sookmyung Women's University) ;
  • Chun, Mi-Son (Department of Radiation Oncology, Ajou University School of Medicine) ;
  • Kang, Seung-Hee (Department of Radiation Oncology, Ajou University School of Medicine) ;
  • Kim, Hyun-Sook (Division of Biological Science, College of Science, Sookmyung Women's University)
  • 김경옥 (숙명여자대학교 생명과학부 식품영양과학) ;
  • 전미선 (아주대학교 의과대학 방사선종양학과) ;
  • 강승희 (아주대학교 의과대학 방사선종양학과) ;
  • 김현숙 (숙명여자대학교 생명과학부 식품영양과학)
  • Published : 2009.10.31
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Abstract

Most cancer patients are treated with surgery, chemotherapy or radiation as anticancer therapies. Especially in the case of radiation, these treatments produce adverse effects such as vomiting, weight loss, anorexia, normal cell damage and malabsorption. The major goal of this study was to determine the effect of irradiation on the nutritional and immune status in irradiated rats. A secondary goal was to determine the effectiveness of high protein diet (HP) and resveratrol (Res) in minimizing the adverse effects of radiation. Rats were divided into four groups: normal diet (NP), HP, NP + Res and HP + Res groups. Each group was further divided into subgroups that received radiation (RT group) and one that did not (non-RT group). Each diet was supplied from $12^{th}$ day prior to irradiation treatment with irradiation dose of 17.5 Gy. The diets were continued until 10th day after radiation treatment and animals were sacrificed. The radiation treatment showed decreased body weight, serum protein and HDL levels and increased TG and LDL levels in nutritional status. HP, NP + Res and HP + Res groups reduced the level of serum LDL and TG in irradiated rats. NP + Res and HP + Res groups increased reduced albumin level of serum in RT group. In case of immune status, the radiation treat-ment showed decreased WBC, lymphocytes and increased neutrophil and eosinophil levels. The levels of serum IL-2 and IL-6 were significantly increased by radiation, however the cytokine levels decreased in all dietary treatment groups. These results showed that high protein diet and resveratrol supplementation seem to minimize the adverse effects of radiation on lipid nutritional status and inflammation response in the rat model.

방사선 치료는 대부분의 암환자 치료에 적용되고 있다. 그러나 방사선 조사로 인한 부작용으로 환자들은 고통을 받고 있으며, 이러한 방사선치료의 부작용을 줄이고 환자의 영양상태 및 면역기능을 강화시킴으로써 암을 이겨낼 수 있는 능력을 강화시키는 것은 매우 중요한 일이다. 따라서 본 연구에서는 방사선 조사로 염증이 유도된 흰쥐를 사용하여 고단백식이와 레스베라트롤을 식이에 첨가하였을 경우 영양상태와 면역지표에 변화가 있는지를 조사하여 다음과 같은 결론을 얻었다. 1) 방사선 조사로 인한 체중 감소 현상이 모든 군에서 뚜렷하게 나타났다. 정상식이 섭취량에 있어서는 방사선조사 전에는 모든 군에서 유사하게 나타났으나 방사선 치료후 유의하게 식이섭취가 감소하였다. 증가된 IL-6는 leptin의 분비와 단백질 분해를 야기시키고 이로 인하여 식욕감 퇴로 이어져 체중감소가 생긴다. 2) 본 연구에서는 방사선 조사로 인한 영양상태 변화를 살펴 본 결과 혈중 총 단백질과 알부민 농도가 모든 군에서 현저하게 감소하였으며, 레스베라트롤을 식이에 첨가하였을 경우 식이단백질 수준에 상관없이 혈중 알부민 농도를 증가시켰다. 3) 방사선 조사로 인하여 혈중 중성지방 농도가 증가하였으며, LDL-cholesterol 농도의 증가와 함께 HDL-cholesterol 농도가 감소됨으로써 지질 영양상태는 매우 불량한 상태를 초래하였다. 이 경우 단백질 섭취 수준을 높이거나 식이에 레스베라트롤을 첨가한 모든 군에서 혈중 중성지방 농도를 유의하게 감소시킬 수 있었으며, 특히 레스베라트롤을 첨가함으로써 혈중 LDL-cholesterol 수준도 유의하게 낮출 수 있는 것으로 나타났다. 3) 방사선 조사에 따른 혈액세포 수의 변화에 있어서는 유의한 백혈구의 증가와 혈소판의 감소를 들 수 있는데 고단백과 레스베라트롤을 동시에 처리한 군에서 백혈구수를 정상대조군 수준으로 낮출 수 있었으며, 감소된 혈소판 농도는 식이단백질 수준을 높임으로써 정상대조군 수준으로 회복시킬 수 있었다. 4) 또한 본 실험에서는 방사선조사에 의한 백혈구 백분율 변화에 있어서도 림프구의 백분율이 현저하게 감소한 반면 중성구와 호산구의 비율은 유의하게 증가하였다. 이 경우 레스베라트롤 첨가와 무관하게 단백질 섭취 수준을 높임으로써 방사선 조사에 의한 호산구 비율의 증가를 유의하게 낮출 수 있었다. 5) 방사선조사에 의하여 혈중 사이토카인 농도에도 변화가 있었다. 가장 현저한 변화로는 방사선 조사에 의하여 혈중 IL-2와 IL-6의 농도가 유의하게 증가하였으며 두 경우 모두에서 단백질 수준을 높이거나 레스베라트롤을 첨가함으로써 사이토카인의 농도를 정상대조군 수준으로 회복 시켰다. 또한 방사선 조사에 의하여 혈중 IL-12 농도가 현저히 감소하였는데 레스베라트롤을 첨가함으로써 식이단백질 수준에 상관없이 정상대조군 수준으로 증가시켰다. 본 연구의 결과를 통해 항암치료를 위하여 널리 사용되고 있는 방사선 조사는 환자의 영양상태와 면역능에 있어 다양한 변화를 초래함으로써 부작용을 야기시키는 것으로 확인되었다. 이 경우 식이의 단백질 수준을 높이거나 식이에 적정 수준의 레스베라트롤을 첨가함으로써 저하된 환자의 기능을 증진시켜 영양불균형을 개선함과 동시에 면역기능을 높여 염증반응의 부작용을 완화시킬 수 있는 것으로 나타났다.

Keywords

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