Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of Leucine Intake on Body Weight Reduction in Rats Fed High Fat Diet

Leucine의 섭취가 고지방 식이를 섭취한 흰쥐의 체중 감소에 미치는 영향

  • Park, Hoon-Jung (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Lee, Eun-Ju (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kim, Joo-Hee (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kim, Ji-Yeon (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kwon, O-Ran (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kim, Mi-Kyung (Department of Nutritional Science and Food Management, Ewha Womans University)
  • 박훈정 (이화여자대학교 식품영양학과) ;
  • 이은주 (이화여자대학교 식품영양학과) ;
  • 김주희 (이화여자대학교 식품영양학과) ;
  • 김지연 (이화여자대학교 식품영양학과) ;
  • 권오란 (이화여자대학교 식품영양학과) ;
  • 김미경 (이화여자대학교 식품영양학과)
  • Published : 2009.12.31
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Abstract

The principal objective of this study was to determine the effects of leucine on body weight reduction in high fat diet-induced overweight rats. To induce overweight, six-month-old male Sprague-Dawley rats (n = 80) were divided into 8 groups; one group of 10 rats was fed on a normal fat diet and the remaining 70 rats were fed on a high-fat diet (40% of energy as fat) for 14 weeks. Then, 10 rats fed on the normal fat diet and another 10 rats fed on the high fat diet were sacrificed to identify overweight induction. The remaining 60 rats were divided randomly into 6 groups according to body weight and fed on one of the diets with different dietary fat levels (9.6% or 40% of energy as fat) and leucine levels (0, 0.6 or 1.2 g/kg BW) for the following 5 weeks of experiments. The body weight loss in the Leu-administered groups (0.6 g, 1.2 g/kg BW) was significantly higher than those of Leu non-administered groups. The perirenal fat pad weights in the Leu-administered groups were significantly lower than those of the Leu non-administered groups. Of the hepatic enzymes, glucose-6-phosphate dehydrogenase (G6PDH) activities were reduced significantly in the Leu-administered groups than in the Leu non-administered groups. With the oral glucose tolerance test (OGTT), the incremental areas under the curve of the glucose response (IAUC) of the Leu-administered groups were significantly lower than those of the Leu non-administered groups. The fasting glucose concentration and HOMA-IR of the Leu-administered groups were significantly lower than those of the Leu non-administered groups. In conclusion, the results of this study suggest that one of the possible mechanisms of leucine in the observed body weight reduction might involve the inhibition of lipogenic enzyme activities such as glucose-6-phosphate dehydrogenase, rather than the activation of lipolysis enzymes. Additionally, leucine adminstration resulted in improved glucose metabolism.

Keywords

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