Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Optimal Extraction Condition and Characterization of Antidementia Acetylcholinesterase Inhibitor from Job's Tears (Coix lachrymajobi L.)

율무로부터 항치매성 Acetylcholinesterase 저해물질의 최적추출 조건 및 특성

  • Seo, Dong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Jang, Jeong-Hoon (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Kim, Na-Mi (KT&G Central Research Institute) ;
  • Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
  • 서동수 (배재대학교 생명유전공학과) ;
  • 장정훈 (배재대학교 생명유전공학과) ;
  • 김나미 ((주) KT&G 중앙 연구원) ;
  • 이종수 (배재대학교 생명유전공학과)
  • Published : 2009.12.30
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Abstract

For the development of a new antidementia functional food or alternative drug using agricultural products, Job's tears (Coix lachrymajobi L.), which shows high acetylcholinesterase (AChE) inhibitory activity (55.1%) was selected and the extraction conditions of AChE inhibitor were optimized. AChE inhibitor of Job's tears was maximally extracted when it was treated with 60% methanol at $40^{\circ}C$ for 6 h. The AChE inhibitor of the methanol extracts was partially purified by systematic solvent extraction, thin layer chromatography, silica gel chromatography and reverse-phase HPLC and the partial purified AChE inhibitor with inhibitory activity ($IC_{50}$) of $0.608\;{\mu}g$ was obtained. The partial purified AChE inhibitor was soluble in methanol and hexane, and insoluble in water. Its maximum absorption spectra was 230 nm and also it was stable in the range of $30^{\circ}C$ and $70^{\circ}C$ and pH 4.0-8.0 for 1 h.

Keywords

References

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