Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Phytochemical Constituents of Thesium chinense TURCZ and Their Cytotoxic Activities In Vitro

  • Lee, Il-Kyun (Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University) ;
  • Kim, Ki-Hyun (Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University) ;
  • Choi, Sang-Un (Research Institute of Chemical Technology) ;
  • Lee, Jae-Hyun (College of Oriental Medicine, Dongguk University) ;
  • Lee, Kang-Ro (Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University)
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Column chromatographic separation of the MeOH extract from the aerial parts of Thesium chinense TURCZ led to the isolation of two norsesquiterpenes (1 - 2), two phenylpropanes (3 - 4) and four flavonoids (5 - 8). Their structures were determined by spectroscopic means to be 5,6-epoxy-3-hydroxy-7-megastigmen-9-ene (1), (-)-loliolide (2), methyl-p-hydroxycinnamate (3), methyl caffeate (4), kaempferol (5), kaempferol-3-O-${\beta}$-Dglucopyranoside (6), kaepmferol-3,7-di-O-${\beta}$-D-glucopyranoside (7) and kaempferol-3-O-${\beta}$-D-glucopyranoside-6''-(3-hydroxy-3-methylglutarate) (8). Compounds 1 - 4, 7 and 8 were first isolated from this source. The isolated compounds were evaluated for their cytotoxicty in vitro using the sulforhodamin B bioassay (SRB).

Keywords

References

  1. Ahn, D.K., Illustrated Book of Korean Medicinal Herbs, Kyo-hak Publishing Co., p.165 (1998)
  2. Berhow, M.A., Bennett, R.D., Poling, S.M., Vannier, S., Hidaka, T., and Omura, M., Acylated flavonoids in dallus cultures of Citrus aurantifolia. Phytochemistry, 36, 1225-1227, (1994) https://doi.org/10.1016/S0031-9422(00)89641-7
  3. Choi, S.Z., Choi, S.U., and Lee, K.R., Phytochemical constituents of the aerial parts from Solidago virga-aurea var. gigintea. Arch. Pharm. Res., 27, 164-168, (2004) https://doi.org/10.1007/BF02980100
  4. Do, J.C., Uu, Y.J., Jung, K.Y., and Son, K.H., Flavonoids from the leaves of Polygala japonica. J. Kor. Chem. Soc., 23, 9-13, (1992)
  5. Jung, K.Y., Do, J.C., and Son, K.H., Kaempferol-3-O-[6$^{\prime}$$^{\prime}$-O-(3-hydroxy-3-methylglutaroyl)-glucoside] from leaves of Polygala japonica. Phytochemistry, 34, 1196-1197, (1993) https://doi.org/10.1016/S0031-9422(00)90747-7
  6. Kim, K.H, Lee, K.H., Choi, S.U., Kim, Y.H., and Lee, K.R., Terpene and phenolic constituents of Lactuca indica L. Arch. Pharm. Res., 31, 983-988, (2008) https://doi.org/10.1007/s12272-001-1256-8
  7. Lee, J.H., Ku, C.H., Baek, N.I., Kim, S.H., Park, H.W., and Kim, D.K., Phytochemical constituents from Diodia teres. Arch. Pharm. Res., 27, 40-43, (2004) https://doi.org/10.1007/BF02980043
  8. Lu, Y. and Wang, J., Study on chemical constituents of Thesium chinensis. Zhongcaoyao, 35, 491-493 (2004)
  9. Park, K.E., Kim, Y.A., Jung, H.A., Lee, H.J., Ahn, J.W., Lee, B.J., and Seo, Y.W., Three norisoprenoids from the brown alga Sargassum thunvergii. J. Kor. Chem. Soc., 48, 394-398, (2004) https://doi.org/10.5012/jkcs.2004.48.4.394
  10. Skehan, P., Stroreng, R., Scudiero, D., Monks, A., Mcmahon, J., Vistica, D., Warren, J. T., Bokesch, H., Kenney, S., and Boyd, M.R., New colorimetric cytotoxicity assay for anticacer-drug screening. J. Natl. Cancer Inst., 82, 1107-1112 (1990) https://doi.org/10.1093/jnci/82.13.1107
  11. Wang, Z. and Li, S.S., Isolation and identification of alkaloid from Thesium chinense Turcz. Chinese J. Med.Chem., 16, 306-308, (2006)