The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Inhibitory Effect of Taraxaci Herba Extract (THE) on Pro-inflammatory Mediatory

포공영(蒲公英)의 염증성 사이토카인 발현 및 조절에 관한 연구

  • Noh, Kyung-Ho (Department of pediatrics, College of Oriental Medicine, Daegu Haany University) ;
  • Baek, Jung-Han (Department of pediatrics, College of Oriental Medicine, Daegu Haany University)
  • 노경호 (대구한의대학교 한의과대학 소아과학교실) ;
  • 백정한 (대구한의대학교 한의과대학 소아과학교실)
  • Published : 2009.12.31
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Abstract

Objectives The purpose of this study is to find out the effect of Taraxaci Herba Extract (THE), LPS, on pro-inflammatory mediatory. Methods After the treatment of Taraxaci Herba MeOH extract dissolved in EMEM for 1 hour prior to the addition of LPS ($1\;{\mu}g/ml$), cell viability was measured by MTT assay, Nitric Oxide production was monitored by measuring the nitrite content in culture medium. And levels of cytokine and PGE2 were analyzed by sandwich immunoassays. Results THE inhibited the production of nitrite and nitrate (0.03 and 0.1 mg/ml), TNF-$\alpha$, (0.03 and 0.1 mg/ml), IL-$1{\beta}$(0.03 and 0.1 mg/ml), IL-6 (0.01, 0.03 and 0.1 mg/ml) and PGE2(0.03 and 0.1 mg/ml) activated with LPS. In Raw 264.7 cells activated with lipopolysaccharide. Conclusions According to the results above, Taraxaci Herba can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.

Keywords

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