대한본초학회지 (The Korea Journal of Herbology)

  • 제24권4호
  • /
  • Pages.121-126
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  • 2009
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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쑥부쟁이 추출물의 아세틸콜린에스테라제 저해 및 항산화 활성

Acetylcholinesterase Inhibitory and Antioxidant Properties of Aster yomena Extract

  • 배종섭 (대구한의대학교 한방제약공학과) ;
  • 김태훈 (대구한의대학교 한약재약리학과)
  • Bae, Jong-Sup (Department of Herbal Pharmaceutical Engineering, Daegu Haany University) ;
  • Kim, Tae-Hoon (Department of Herbal Medicinal Pharmacology, Daegu Haany University)
  • 발행 : 2009.12.30
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초록

Objectives : To evaluate the radical scavenging and acetylcholinesterase (AChE) inhibitory activities of the ethylacetate (EtOAc)-soluble portion of a methanolic extract of Aster yomena, three different assay systems were performed. Methods : The antioxidant activity of A. yomena extract was tested as its capacity to scavenging free radicals of DPPH and $ABTS^+$, which has been widely used to evaluate the antioxidant activity of natural products from plant sources. AChE inhibitory activity was tested against mouse brain AChE by spectrophotometric method of Ellman using ELISA microplate reader. Results : The methanolic extract of A. yomena was fractionated and the EtOAc-soluble portion showed significant AChE inhibitory and free radical scavenging effects. Also the EtOAc-soluble portion revealed the highest phenolic contents as compared to the other extracts. Conclusions : These results indicate that phenolic compounds may be important constituents that give rise to the anti-AChE and antioxidative activities of A. yomena extract. Further phytochemical studies on this plant, for nutraceutical or pharmaceutical application, are warranted.

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