The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Inhibitory Effect of Coicis Semen Extract(CSE) on Pro-inflammatory Mediatory

의이인(薏苡仁)의 염증성 사이토카인 발현 및 조절에 관한 연구

  • Yun, Hye-Jin (Department of Pediatrics, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Yu-Jin (Department of Pediatrics, College of Oriental Medicine, Daegu Haany University) ;
  • Kang, Mi-Sun (Department of Pediatrics, College of Oriental Medicine, Daegu Haany University) ;
  • Baek, Jung-Han (Department of Pediatrics, College of Oriental Medicine, Daegu Haany University)
  • 윤혜진 (대구한의대학교 한의과대학 소아과학교실) ;
  • 이유진 (대구한의대학교 한의과대학 소아과학교실) ;
  • 강미선 (대구한의대학교 한의과대학 소아과학교실) ;
  • 백정한 (대구한의대학교 한의과대학 소아과학교실)
  • Published : 2009.04.30
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Abstract

Objectives This study was evaluated the effects of CSE the regulatory mechanism of NO and cytokines in the LPS-stimulated Raw 264.7 cells. Methods The Coicis Semen MeOH extract dissolved in EMEM for 1 hour prior to the addition of LPS(1${mu}g/ml$). The cell viability was measured by MTT assay, and Nitric Oxide production was monitored by measuring the nitrite content in culture medium. The levels of cytokine and PGE2 were analyzed by sandwich immunoassays. Results CSE inhibited the production of NO (0.03 and 0.1 mg/ml), $TNF-{\alpha}$ (0.03 and 0.1 mg/ml), $IL-1{\beta}$ (0.03 and 0.1 mg/ml), IL-6 (0.03, 0.1 mg/ml) and PGE2(0.03 and 0.1 mg/ml) in Raw 264.7 cells activated with LPS(lipopolysaccharide). Conclusion According to the results above, Coicis Semen can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.

Keywords

References

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