한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제17권2호
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  • Pages.133-138
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  • 2009
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

연잎추출물의 B형 간염 표면 항원 발현 억제 효과

Nelumbo nucifera Leaves Extract Reduced the Production of Hepatitis B Surface Antigen on HepG2.2.15

  • 이윤희 (전남대학교 응용생물공학부) ;
  • 강이중 (전남대학교 응용생물공학부) ;
  • 이성진 (전남대학교 응용생물공학부)
  • Lee, Yun-Hee (Department of Biotechnology (BK21 program), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kang, Li-Jung (Department of Biotechnology (BK21 program), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Lee, Seong-Gene (Department of Biotechnology (BK21 program), Division of Applied Bioscience and Biotechnology, Chonnam National University)
  • 발행 : 2009.04.30
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초록

Nelumbo nucifera (lotus) is known to be a useful medicinal plant and leaf extract contains several flavonoids and alkaloids. To analyze the effect of Nelumbo nucifera leaves extract (NNL) on the HBsAg production, we treated NNL on HepG2.2.15 cells which contain the hepatitis B viral genome and secrete surface antigen into media. NNL suppressed the production of hepatitis B surface antigen as a dose-dependent manner. To analyze the effect of NNL on HBV DNA replication, PCR analysis was performed. NNL was not affected the HBV DNA replication and HBsAg mRNA expression. To understand the effect of NNL on the production of HBsAg, we carried out the analysis of lipid-metabolizing gene expression using one-step RT-PCR. NNL reduced the gene expression of FASN and SREBP2 and increased the expression of LDLR. Triglyceride content of HepG2.2.15 cells was not decreased by treatment of NNL. This result suggests a possibility that NNL may have an effect for the inhibition of hepatitis B surface antigen by modulation of lipid and cholesterol metabolism.

키워드

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