Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Nelumbo nucifera Leaves Extract Reduced the Production of Hepatitis B Surface Antigen on HepG2.2.15

연잎추출물의 B형 간염 표면 항원 발현 억제 효과

  • Lee, Yun-Hee (Department of Biotechnology (BK21 program), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Kang, Li-Jung (Department of Biotechnology (BK21 program), Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Lee, Seong-Gene (Department of Biotechnology (BK21 program), Division of Applied Bioscience and Biotechnology, Chonnam National University)
  • 이윤희 (전남대학교 응용생물공학부) ;
  • 강이중 (전남대학교 응용생물공학부) ;
  • 이성진 (전남대학교 응용생물공학부)
  • Published : 2009.04.30
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Abstract

Nelumbo nucifera (lotus) is known to be a useful medicinal plant and leaf extract contains several flavonoids and alkaloids. To analyze the effect of Nelumbo nucifera leaves extract (NNL) on the HBsAg production, we treated NNL on HepG2.2.15 cells which contain the hepatitis B viral genome and secrete surface antigen into media. NNL suppressed the production of hepatitis B surface antigen as a dose-dependent manner. To analyze the effect of NNL on HBV DNA replication, PCR analysis was performed. NNL was not affected the HBV DNA replication and HBsAg mRNA expression. To understand the effect of NNL on the production of HBsAg, we carried out the analysis of lipid-metabolizing gene expression using one-step RT-PCR. NNL reduced the gene expression of FASN and SREBP2 and increased the expression of LDLR. Triglyceride content of HepG2.2.15 cells was not decreased by treatment of NNL. This result suggests a possibility that NNL may have an effect for the inhibition of hepatitis B surface antigen by modulation of lipid and cholesterol metabolism.

Keywords

References

  1. Eberl$\acute{e}$ D, Hegarty B, Bossard P, Ferre P and Foufelle F. (2004). SREBP transcription factors: master regulator of lipid homeostasis. Biochimie. 86:839-848 https://doi.org/10.1016/j.biochi.2004.09.018
  2. Gavilanes F, Gonzalez-Ros JM and Peterson DL. (1982). Structure of hepatitis B surface antigen. Characterization of the lipid components and their association with the viral proteins. Journal of Biological Chemistry. 257:7770-7777
  3. Jeong S, Yim HW, Bae SH and Lee W. (2008). Changes of hepatitis B surface antigen seroprevalence in Korea, 1998-2005. Korean Journal of Epidemiology. 30:119-127 https://doi.org/10.4178/kje.2008.30.1.119
  4. Jung HA, Jung YJ, Yoon NY, Jeong DM, Bae HJ, Kim D, Na DH and Choi JS. (2008). Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase advanced glycation end products formation, and oxidative stress. Food and Chemical Toxicology. 46:3818-3826 https://doi.org/10.1016/j.fct.2008.10.004
  5. Kashiwada Y, Aoshima A, Ikeshiro Y, Chen YP, Furukawa H, Itoigawa M, Fujioka T, Mihashi K, Cosentino LM, Morris-Natshke SL and Lee KH. (2005). Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids. Bioorganic and Medicinal Chemistry. 13:443-448 https://doi.org/10.1016/j.bmc.2004.10.020
  6. Kim JH, Kim YS and Surh DJ. (1994). The prevalence of HBsAg and anti-HCV in Korean patients with hepatocellular carcinoma. Korean Journal of Medicine. 46:181-190
  7. Kim TG, Park MS, Han H, Kang SY, Jung KK, Rheu HM and Kim SH. (1999) Inhibitory effects of Terminalia chebula, Sanguisorba officinalis, Rubus coreanus and Rheum palmatum on hepatitis B virus replication in HepG2.2.15 cells. Yakhak Hoeji. 43:458-463
  8. Kong WJ, Liu J and Jiang JD. (2006). Human low-density lipoprotein receptor gene and its regulation. Journal of Molecular Medicine. 84:29-36 https://doi.org/10.1007/s00109-005-0717-6
  9. Korba BE and Gerin JL. (1992). Use of a standardized cell culture assay to assess activities of nucleoside analogs against hepatitis B virus replication. Antiviral Research. 19:55-70 https://doi.org/10.1016/0166-3542(92)90056-B
  10. Korba BE and Milman G. (1991). A cell culture assay for compounds which inhibit hepatitis B virus replication. Antiviral Research. 15:217-228 https://doi.org/10.1016/0166-3542(91)90068-3
  11. Kuo YC, Lin YL, Liu CP and Tsai WJ. (2005) Herpes simplex virus type 1 propagation in HeLa cells interrupted by Nelumbo nucifera. Journal of Biomedical Science. 12:1021-1034 https://doi.org/10.1007/s11373-005-9001-6
  12. Lin YL, Shiao MS, Mettling C and Chou CK. (2003). Cholesterol requirement of hepatitis B surface antigen (HBsAg) secretion. Virology. 314:253-260 https://doi.org/10.1016/S0042-6822(03)00403-3
  13. Merican I, Guan R, Amarapuka D, Alexander MJ, Chutaputti A, Chien RN, Hasnian SS, Leung N, Lesmana L, Phiet PH, Sjalfoellah Noer HM, Sollano J, Sun HS and Xu DZ. (2000). Chronic hepatitis B virus infection in Asian countries. Journal of Gastroenterology and Hepatology. 15:1356-1361 https://doi.org/10.1046/j.1440-1746.2000.0150121356.x
  14. Mukherjee PK, Saha K, Pal M and Saha BP. (1997). Effect of Nelumbo nucifera rhizome extract on blood sugar level in rats. Journal of Ethnopharmacology. 58:207-213 https://doi.org/10.1016/S0378-8741(97)00107-4
  15. Ohkoshi E, Miyazaki H, Shindo K, Watanabe H, Yoshida A and Yajima H. (2007). Constituents from the leaves of Nelumbo nucifera stimulate lipolysis in the white adipose tissue of mice. Planta Medica. 73:1255-1259 https://doi.org/10.1055/s-2007-990223
  16. Ono Y, Hattori E, Fukaya Y, Imai S and Ohizumi Y. (2006). Anti-obesity effect of Nelumbo mucifera leaves extract in mice and rats. Journal of Ethnopharmacology. 106:238-244 https://doi.org/10.1016/j.jep.2005.12.036
  17. Wu MJ, Wang L, Weng CY and Yen JH. (2003). Antioxidant activity of methanol extract of the lotus leaf (Nelumbo nucifera Gernt.). American Journal of Chinese Medicine. 31:687-698 https://doi.org/10.1142/S0192415X03001429
  18. Yuk CS. (1990) Coloured medicinal plants of Korea. Academy Book Co., Seoul Korea. pp. 219-230