Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High frequency plant regeneration from transverse thin cell layers in Indian mustard (Brassica juncea L.)

  • Bhuiyan, Mohammed Shafi Ullah (Plant Physiology and Cell Biotechnology Laboratory, Department of Agricultural Biology, Chungnam National University) ;
  • Lim, Yong-Pyo (Department of Horticulture, Chungnam National University) ;
  • Min, Sung-Ran (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Choi, Kwan-Sam (Plant Physiology and Cell Biotechnology Laboratory. Department of Agricultural Biology, Chungnam National University) ;
  • Liu, Jang-R. (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Published : 2009.03.31
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Abstract

An efficient and reproducible plant regeneration system was established using transverse thin cell layers (tTCLs) in five cultivars of Brassjca juncea L. The effects of medium conditions, explant types (tTCLs of hypcotyl and cotyledonary petiole) on shoot regeneration were examined in this study. The maximum shoot regeneration frequency was obtained in Murashige and Skoog (MS) medium supplemented with 4 mg/L 6-benzylaminopurine (BA) and 0.2 mg/L 1-naphthaleneacetic acid (NAA). The hypocotyls derived tTCL explants had more shoot regeneration frequency (52%) than the cotyledonary petiole derived tTCL explants. Shoot induction was further improved by the addition of silver nitrate ($AgNO_3$) in the regeneration medium. A significant genotypic effect was also observed between the five cultivars; Rai-5 displayed higher capacities to produce shoots than other cultivars. Regenerated shoots were rooted on MS basal medium without PGRs which induced 90% of roots. The plantlets established in greenhouse conditions with 99% survival, flowered normally and set seeds. The regenerated plants were fertile and identical to source plants.

Keywords

References

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Cited by

  1. Factors for high frequency plant regeneration in tissue cultures of Indian mustard (Brassica juncea L.) vol.36, pp.2, 2009, https://doi.org/10.5010/JPB.2009.36.2.137