Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Identification and characterization of a rice blast fungal elicitor-inducible Oshin1 gene

  • Kim, Cha-Young (Bioindustry Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sung-Ho (Department of Biology, Division of Applied Life Science (BK21), PMBBRC and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In order to understand the molecular interactions that occur between rice and the rice blast fungus during infection, we previously identified a number of rice blast fungal elicitor-responsive genes from rice (Oryza sativa cv. Milyang 117). Here, we report the cloning and characterization of the rice fungal elicitor-inducible gene Oshin1 (GenBank Accession Number AF039532). Sequence analysis revealed that the Oshin1 cDNA is 1067 bp long and contains an open reading frame encoding 205 amino acid residues. The Oshin1 gene shows considerable sequence similarity to the tobacco hin1 and hin2 genes. The predicted Oshin1 protein has a cysteine-rich domain at the N-terminus and is rich in leucine, serine, and alanine residues. Southern blot analysis suggests that Oshin1 gene is a member of a small gene family in the rice genome. To examine the expression of Oshin1, Northern blot analysis was conducted. Expression of the Oshin1 transcript is rapidly induced in suspension-cultured rice cells treated with fungal elicitor, salicylic acid or hydrogen peroxide. In addition, Oshin1 transcript levels are rapidly increased by treatment with $Ca^{2+}$/A23187. The expression of Oshin1 was also elevated in 3-week old leaf tissues upon ethephon application or fungal elicitor treatment. Our results suggest that the Oshin1 gene is involved in plant defense responses to environmental stresses.

Keywords

References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-410 https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Church GM, Gilbert W (1984) Genomic sequencinq. Proc Natl Acad Sci USA 81:1991-1995 https://doi.org/10.1073/pnas.81.7.1991
  3. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19-21 https://doi.org/10.1007/BF02712670
  4. Dixon RA, Lamb CJ (1990) Molecular communication in interactions between plant and microbial pathogens. Annual Rev Pl Physiol Pl Molec Biol 41:339-367 https://doi.org/10.1146/annurev.pp.41.060190.002011
  5. Doke N, Furuichi N (1982) Response of protoplasts to hyphal wall components in relationship to resistance of potato to Phytophthora infestans. Plant Pathol 2:23-30
  6. Dygert SL, Li LH, Florida D, Thoma J (1965) Determination of reducing sugar with improved precision. Anal Biochem 13:367-374 https://doi.org/10.1016/0003-2697(65)90327-1
  7. Ebel J, Bhagwat A, Cosio EG, Feger M, Kissel U, Mithofer A, Waldmuller T (1994) Elicitor-binding proteins and signal transduction in the activation of a phytoalexin defense response. Can J Bot 37:S506-S510
  8. Gopalan S, Wei W, He SY (1996) hrp gene-dependent induction of hin1: a plant gene activated rapidly by both harpins and avrPto gene-mediated signal. Plant J 10:591-600 https://doi.org/10.1046/j.1365-313X.1996.10040591.x
  9. Grbi V, Bleecker AB (1995) Ethylene regulates the timing of leaf senescence in Arabidopsis. Plant J 8:595-602 https://doi.org/10.1046/j.1365-313X.1995.8040595.x
  10. Kim CY, Gal SW, Choe MS, Jeong SY, Lee SI, Cheong YH, Lee SH, Choi YJ, Han C-d, Kang KY, Cho MJ (1998) A new class II rice chitinase, Rcht2, whose induction by fungal elicitor is abolished by protein phosphatase 1 and 2A inhibitor. Plant Mol Biol 37:523-534 https://doi.org/10.1023/A:1005960313459
  11. Kim WY, Kim CY, Cheong NE, Choi YO, Lee KO, Lee S-H, Park JB, Nakano A, Bahk JD, Cho MJ, Lee SY (1999) Characterization of two fungal-elicitor-induced rice cDNAs encoding functional homologues of the rab-specific GDP-dissociation inhibitor. Planta 210:143-149 https://doi.org/10.1007/s004250050663
  12. Kim CY, Koo YD, Jin JB, Moon BC, Kang CH, Kim ST, Park BO, Lee SY, Kim ML, Hwang I, Kang KY, Bahk JD, Lee SY, Cho MJ (2003) Rice C2-domain proteins are induced and translocated to the plasma membrane in response to a fungal elicitor. Biochemistry 42:11625-11633 https://doi.org/10.1021/bi034576n
  13. Kim CY, Lee S-H, Park HC, Bae CG, Cheong YH, Choi YJ, Han CD, Lee SA, Lim CO, Cho MJ (2000) Identification of rice blast fungal elicitor-responsive genes by differential display analysis. Mol Plant-Microbe Interact 13:470-474 https://doi.org/10.1094/MPMI.2000.13.4.470
  14. Lagrimini LM, Burkhart W, Moyer M, Rothstein S (1987) Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression. Proc Natl Acad Sci USA 84:7542-7546 https://doi.org/10.1073/pnas.84.21.7542
  15. Lee WY, Hong JK, Kim CY, Chun HJ, Park HC, Kim JC, Yun DJ, Chung WS, Lee S-H, Lee SY, Cho MJ, Lim CO (2003) Over-expressed rice ADP-nbosylation factor 1 (RARFl) induces pathogenesis-related genes and pathogen resistance in tobacco plants. Physiologia Plantarum 119:573-581 https://doi.org/10.1046/j.1399-3054.2003.00215.x
  16. Nurnberger T, Nennstiel D, Jabs T, Sacks WR, Hahlbrock K, Scheel D (1994) High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses. Cell 78:449-460 https://doi.org/10.1016/0092-8674(94)90423-5
  17. Ohira K, Ojima K, Fujiwara A (1973) Studies on the nutrition of rice cell culture. I. A simple, defined medium for rapid growth in suspension culture. Plant Cell Physiol 14:1113-1121
  18. Pontier D, Gan S, Amasino RM, Roby D, Lam E (1999) Markers for hypersensitive response and senescence show distinct patterns of expression. Plant Mol Biol 39:1243-1255 https://doi.org/10.1023/A:1006133311402
  19. Rakwal R, Agrawal GK, Tamogami S, Iwahashi H (2004) Transcriptional profiling of Oshin 1 in rice plants: a potential role in defense/stress and development. Plant Sci 166:997-1005 https://doi.org/10.1016/j.plantsci.2003.11.031
  20. Raymond P, Farmer EE (1998) Jasmonate and salicylate as global signals for defense gene expression. Curr Opin Plant Biol 1:404-411 https://doi.org/10.1016/S1369-5266(98)80264-1
  21. Rusterucci C, Stallaert V, Milat ML, Pugin A, Ricci P, Blein JP (1996) Relationship between active oxygen species, lipid pcroxidation, necrosis, and phytoalexin production induced by elicitins in Nicotiana. Plant Physiol 111:885-891 https://doi.org/10.1104/pp.111.3.885
  22. Ryan CA, Farmer EE (1991) Oligosaccharide signals in plants: a current assessment. Annu Rev Plant Physiol Plant Mol Biol 42:651-674 https://doi.org/10.1146/annurev.pp.42.060191.003251
  23. Varet A, Parker JE, Tornero P, Nass N, Nurnberger T, Dangl JL, Scheel D, Lee J (2002) NHL25 and NHL3, two NDRl/HIN1-like genes in Arabidopsis thaliana with potential role(s) in plant defense. Mol Plant-Microbe Interact 15:608-616 https://doi.org/10.1094/MPMI.2002.15.6.608
  24. Wei ZM, Laby RJ, Zumoff CH, Bauer DW, He SY, Collmer A, Beer SV (1992) Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science 257:85-88 https://doi.org/10.1126/science.1621099
  25. Willis DK, Rich JJ, Hrabak EM (1991) hrp genes of phytopathogenic bacteria. Mol Plant-Microbe Interact 4:132-138 https://doi.org/10.1094/MPMI-4-132
  26. Yoshida S, Ito M, Nishida I, Watanabe A (2001) Isolation and RNA gel blot analysis of genes that could serve as potential molecular markers for leaf senescence in Arabidopsis thaliana. Plant Cell Physiol 42:170-178 https://doi.org/10.1093/pcp/pce021
  27. Yu LM (1995) Elicitins from Phytophthora and basic resistance in tobacco. Proc Natl Acad Sci USA 92:4088-4094 https://doi.org/10.1073/pnas.92.10.4088
  28. Zacarias L, Reid MS (1990) Role of growth regulators in the senescence of Arabidopsis thaliana leaves. Physiolog Plantarum 80:549-554 https://doi.org/10.1111/j.1399-3054.1990.tb05677.x