한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제13권4호
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  • Pages.313-320
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  • 2009
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

강도다리 Platichthys stellatus 담수 순화시 삼투압 조절에 미치는 갑상선호르몬의 영향

Effects of 3,5,3'-Triiodothyronine (T3) on Osmoregulation following Freshwater Acclimation in Starry Flounder

  • 민병화 (국립수산과학원 육종연구센터) ;
  • 임한규 (국립수산과학원 양식관리과) ;
  • 장영진 (부경대학교 양식학과) ;
  • 김영수 (부경대학교 양식학과) ;
  • 명정인 (국립수산과학원 육종연구센터)
  • Min, Byung-Hwa (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Lim, Han-Kyu (Dept. of Aquaculture Management, National Fisheries Research and Development Institute) ;
  • Chang, Young-Jin (Dept. of Aquaculture, Pukyong National University) ;
  • Kim, Young-Soo (Dept. of Aquaculture, Pukyong National University) ;
  • Myeong, Jeong-In (Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
  • 발행 : 2009.12.31
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초록

본 연구에서는 외인성 T3처리가 강도다리의 담수 순화시 삼투압 조절과 혈중 코티졸 변화에 어떠한 영향을 미치는지 조사하였다. 해수에서 사육 중인 강도다리에 갑상선호르몬의 일종인 T3를 5, 10 및 $15{\mu}g$/g body weight의 농도로 0.9% NaCl과 함께 주입하고 담수로 옮긴 후 3일째 혈액을 채취하였으며, 삼투압 조절 지표인 $Na^+,\;Cl^-$ 및 삼투질 농도와 스트레스 지표인 cortisol, glucose를 조사하였다. $10{\mu}g$/g의 T3를 주입한 실험구의 혈장 $Na^+$와 $Cl^-$의 농도는 호르몬 처리 없이 담수로 이동한 대조구에 비해 유의하게 높았다. 삼투질 농도는 $10{\mu}g$/g과 $15{\mu}g$/g의 농도를 주입한 실험구가 대조구보다 높았다. 그러나 $5{\mu}g$/g 농도의 T3를 주입한 실험구에서는 삼투압 조절에 영향을 미치지 않는 것으로 나타났다. 또한 본 연구에서는 T3를 주입한 모든 실험구에서 glucose와 상관없이 cortisol의 농도가 모두 증가하는 경향을 나타내었다. 이러한 cortisol의 증가는 스트레스 반응이 아닌 삼투압 조절과 관련이 있으며, 결과적으로 강도다리의 담수 순화시 외인 성 T3는 cortisol을 증가시킴으로 어체의 고삼투압 조절 능력을 향상시키는 것으로 추정된다.

The aim of this study was to test if 3,5,3'-triiodothyronine (T3) are involved in the osmoregulatory actions in euryhaline starry flounder Platichthys stellatus. We investigated osmoregulatory parameters ($Na^+,\;Cl^-$ and osmolality), blood cortisol and glucose in starry flounder acclimated to seawater (SW, 33 psu) and that were transferred and allowed to acclimate to freshwater (FW, 0 psu). Fish in SW were injected with T3 (5, 10, and $15{\mu}g$/g body weight) or vehicle (0.9% NaCl), and then transferred to FW. They were sampled 3 days after the transfer. With T3 at $10{\mu}g$/g, levels of plasma $Na^+$ and $Cl^-$ were significantly higher than in sham (only saline) and control fish (without hormone and saline). Osmolality was significantly higher after injection with T3 at 10 and $15{\mu}g$/g than in the control. However, T3 at $5{\mu}g$/g had no effect on hyper-osmoregulation. In this study, all dose of T3 induced a significant increases in plasma cortisol without glucose. These results suggest a positive hyper-osmoregulatory role of T3 in starry flounder to hypoosmotic environment, maybe a positive interaction of T3 with cortisol for maintenance of hyper-osmoregulatory ability.

키워드

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