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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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UVB Protective Effect of Yeast Originated Selenium Peptide on Fibroblast

효모 유래 셀레늄 펩타이드의 인간 섬유아세포에 대한 UVB 보호효과

  • Lee, Hyang-Bok (Department of Biological Engineering, Inha University) ;
  • Lee, Jung-Ok (Department of Biological Engineering, Inha University) ;
  • Nguyen, Dung H. (Department of Biological Engineering, Inha University) ;
  • Yoon, Sun-A (Department of Biological Engineering, Inha University) ;
  • Um, Ji-Min (Department of Biological Engineering, Inha University) ;
  • Lee, Yu-Ri (Department of Biological Engineering, Inha University) ;
  • Moon, Hyung-In (Department of Dermatology, Seoul National University College of Medicine and Institute of Dermatological Science, Seoul National University Hospital) ;
  • Chung, Jin-Ho (Department of Dermatology, Seoul National University College of Medicine and Institute of Dermatological Science, Seoul National University Hospital) ;
  • Kim, Eun-Ki (Department of Biological Engineering, Inha University)
  • Published : 2009.10.29
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Abstract

Selenium-containing peptide (Selenium peptide) was produced by autolysis of Saccharomyces cerevisiae which was cultured in inorganic selenium-supplemented medium. Selenium peptide showed antioxidant activity and protective effects on UVB irradiated human fibroblast. Minimal toxicity of selenium peptide was observed whereas selenium nitrate exhibited cell toxicity as low as $10^{-9}\;M$. Selenium peptide also increased human fibroblast growth, procollagen type I and also decreased MMP-1 (matrix metalloprotease-1). This result showed the potential of selenium peptide as a nontoxic antioxidant.

셀레늄 함유 펩타이드 (셀레늄 펩타이드)는 무기 셀레늄이 포함된 배지에서 효모를 배양하여 효모의 자가분해에 의해 만들었다. 효모 배양에 의해 만들어진 셀레늄 펩타이드는 GPx 유사활성을 보였으며, UVB 조사가 된 인간 섬유아세포에 대하여 세포 보호효과를 나타냈다. 셀레늄 나이트레이트는 $10^{-9}$ 몰 농도에서 낮은 세포독성을 보인반면 셀레늄 펩타이드는 최소의 독성만을 보였다. 또한 셀레늄 펩타이드는 인간 섬유아세포의 성장과 procollagen type I을 증가시킨 반면 MMP-1의 감소를 가져왔다. 연구결과 셀레늄 펩타이드가 무독성의 항산화제로서의 가능성을 보여주었다.

Keywords

References

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