KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Evaluation of Biological and Physico-chemical Detoxification Methods for the Removal of Inhibitors in Lignocellulose Hydrolysate

목질계 바이오매스 가수분해물 중 발효저해 물질에 대한 생물학적 및 물리화학적 무독화 방법의 평가

  • Cho, Dae-Haeng (Department of Chemical Engineering, Kwangwoon University) ;
  • Kim, Yong-Hwan (Department of Chemical Engineering, Kwangwoon University)
  • Published : 2009.10.29
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Abstract

In this study, the detoxification methods were evaluated for the removal of fermentation inhibitors from synthetic solution containing the composition similar to the lignocellulosic hydrolysate. The enzyme peroxidase and laccase were used as a biological treatment method. The physico-chemical methods such as adsorption and ion exchange were applied by using activated charcoal and ion exchange resins. The enzyme peroxidase showed a excellent removal of phenolic compounds. The 5-HMF and furfural were completely removed by activated charcoal. The anion exchange resin showed a good result for detoxification of acetic acid. The activated charcoal and ion exchange resins lead to a loss of sugars more or less. The choice of detoxification method must be made after considering the composition and inhibitors in hydrolysates.

본 연구에서는 리그노셀룰로스 가수분해물과 유사한 조성을 갖는 합성 용액을 이용하여 무독화 실험을 진행하였다. 생물학적 무독화 방법으로는 peroxidase와 laccase와 같은 효소를 이용하였고, 이온교환과 흡착과 같은 물리화학적 방법으로는 이온교환수지와 활성탄을 이용하였다. 효소 중 peroxidase는 페놀계 화합물의 제거에 탁월한 효율을 보였으며, 5-HMF와 furfural은 활성탄에 의해 거의 모두 제거되었고, 아세트산은 음이온교환수지를 사용하는 것이 가장 효율적이었다. 활성탄과 이온교환수지는 다소간의 당손실을 일으켰다. 무독화 방법은 당화액에 포함되어 있는 저해물질의 조성을 고려하여 결정되어야 한다.

Keywords

References

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