Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Histological evaluation of direct pulp capping with DSP-derived synthetic peptide in beagle dog

비글견에서 DSP 유도 합성 펩타이드를 이용한 직접 치수 복조술에 대한 조직학적 연구

  • Kim, Jae-Hoon (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Hong, Jun-Bae (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Lim, Bum-Soon (Department of Dental Biomaterials Science, School of Dentistry, Seoul National University) ;
  • Cho, Byeong-Hoon (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
  • 김재훈 (서울대학교 치의학대학원 치과보존학교실) ;
  • 홍준배 (서울대학교 치의학대학원 치과보존학교실) ;
  • 임범순 (서울대학교 치의학대학원 치과생체재료학교실) ;
  • 조병훈 (서울대학교 치의학대학원 치과보존학교실)
  • Published : 2009.03.31
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Abstract

The purpose of this study was to investigate the pulpal response to direct pulp capping with dentin sialoprotein (DSP) -derived synthetic peptide in teeth of dogs, and to compare its efficacy to capping substances $Ca(OH)_2$ and white mineral trioxide aggregate (WMTA). A total of 72 teeth of 6 healthy male beagle dogs were used. The mechanically exposed pulps were capped with one of the following: (1) DSP-derived synthetic peptide (PEP group): (2) $Ca(OH)_2$ (CH group): (3) a mixture paste of peptide and $Ca(OH)_2$ (PEP+CH group): or (4) white MTA (WMTA group). The access cavity was restored with a reinforced glass ionomer cement. Two dogs were sacrificed at each pre-determined intervals (2 weeks, 1 month, and 3 months). After the specimens were prepared for standard histological processing, sections were stained with hematoxylin and eosin. Under a light microscope, inflammatory response and hard tissue formation were evaluated in a blind manner by 2 observers. In the PEP group, only 3 of 17 specimens showed hard tissue formation, indication that the DSP-derived synthetic peptide did not induce proper healing of the pulp. Compared with the CH group, the PEP group demonstrated an increased inflammatory response and poor hard tissue formation. The CH and WMTA groups showed similar results for direct pulp capping in mechanically exposed teeth of dogs.

본 연구에서는 DSP (dentin sialoprotein)에서 유래된 합성 펩타이드를 동물실험 모델에 적용하여 치수노출 부위에서 상아질 재생을 확인하고, 기존 치 수복조제와의 성능 비교를 통해 새로운 치수복조제로서의 가능성을 확인하고자 하였다. 6마리 비글견의 73개의 치아를 이용하여, 실험적으로 치수를 노출하고 직접 치수 복조술을 시행하였다. 사용한 치수복조제는 (1) $Ca(OH)_2$ (CH군) (2) DSP유도 합성 펩타이드 (PEP군) (3) 합성 펩타이드와 $Ca(OH)_2$ 혼합제 (PEP+CH군) (4) White MTA (WMTA군) 이다. 노출된 치수에 치수복조제를 적용한 후 와동은 강화형 글라스 아이오노머로 충전하였다. 시술 후 2주, 1 개월 및 3개월에 각각 2마리씩 비글견을 희생시키고 조직시편을 제작하였다. 시편은 H&E 염색 후 광학 현미경으로 치수 염증 반응과 경조직 형성 정도를 관찰하였다. PEP군에서는 17개의 시편 중 3 개의 시편에서만 경조직 형성을 관찰할 수 있었으며, 대부분의 시편에서 적절한 치수 회복을 관찰할 수 없었다. PEP군은 CH군에 비해 심한 염증반응을 보이고, 경조직 형성은 불량하였다. CH군과 WMTA군은 기계적으로 노출된 치수에서의 염증반응과 경조직 형성에 있어서 유사한 결과를 보였다.

Keywords

References

  1. Herman BW. Dentin obliteration after treatment with calcium. Zahnarztl Rundschau 39:888-899. 1930. (In German)
  2. Schroder U. Effects of calcium hydroxide-containing pulp-capping agents on pulp cell migration, prolifera tion and differentiation. J Dent Res 64(suppl. 1): 541-548, 1985 https://doi.org/10.1177/002203458506400407
  3. Pitt Ford TR, Torabinejad M, Albedi HR, Bakland LK. Kariyawasam SP. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc 127:1491-1494. 1996 https://doi.org/10.14219/jada.archive.1996.0058
  4. Goldberg M, Six N, Decup F, Lasfargues JJ , Salih E,Tompkins K. Veis A. Bioactive molecules and the future of pulp therapy. Am J Dent 16:66-76, 2003
  5. Schuurs AHB, Gruythuysen RJM, Wesselink PR, Pulp capping with adhesive resin based composite versus calcium hydroxide: a review. Endod Dent Traumatol 16:240-250, 2000 https://doi.org/10.1034/j.1600-9657.2000.016006240.x
  6. Lee SJ , MonsefM, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 19:541-544, 1993 https://doi.org/10.1016/S0099-2399(06)81282-3
  7. Abedi HR, Torabinejad M, Pitt Ford TR. Bakland LK. The use of mineral trioxide aggregate cement as a direct pulp capping material J Am Dent Assoc 127: 1491-1494, 1996
  8. Dominguez MS' Witherspoon DE. Gutmann JL, Opperman LA' Histological and scanning electron microscopy assessment of various vital pulp-therapy materials. J Endod 29:324-333. 2003 https://doi.org/10.1097/00004770-200305000-00003
  9. Iwamoto CE. Adachi E. Pameijer CH, Barnes D, Romberg EE, Jefferies S. Clinical and histological eval uation of white ProRoot MTA in direct pulp capping. Am J Dent 19:85-90, 2006
  10. Goldberg M, Six N, Decup F, et al. Application of bioactive molecules in pulp-capping situations. Adv Dent Res 15:91-95, 2001 https://doi.org/10.1177/08959374010150012401
  11. Decup F. Six N, Palmier B, Buch D. Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar. Clin Oral Invest 4: 110-119. 2000 https://doi.org/10.1007/s007840050126
  12. Six N, Decup F, Lasfargues JJ , Salih E, Goldberg M, Osteogenic proteins (bone sialoprotein and bone morphogenetic protein-7) and dental pulp mineralization. J Mater Sci Mater Med 13:225-232. 2002 https://doi.org/10.1023/A:1013846516693
  13. Wang J , Kennedy JG. Glimcher M,J, Salih E. Novel bioactive property of purified native bone sialoprotein in bone repair of calvarial defect. Trans Orthop Res Soc 23:1007, 1998
  14. George A. Bannon L. Sabsay B, Dillon JW, Malone J, Veis A, Jenkins NA, Gilbert OJ, Copeland NG. The carboxyHerminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J BioI Chern 271:32869-32873. 1996 https://doi.org/10.1074/jbc.271.51.32869
  15. Qin C, Brunn JC , Ridall A. et aI. the expression of dentin sialophosphoprotein gene in bone. J Dent Res 81:392-394, 2002 https://doi.org/10.1177/154405910208100607
  16. Butler WT. Sialoprotein of bone and dentin. J Bio Buccale 19:83-89, 1991
  17. Pitt Ford TR, Torabinejad M, Abedi HR. Bakland LK. Kariyawasam SP. Using mineral trixoide aggregate as a pulp capping material. J Am Dent Assoc 127:1491-1494, 1996 https://doi.org/10.14219/jada.archive.1996.0058
  18. Aeinehchi M, Eslami B, Ghanbariha M, Saffar AS. Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: a preliminary report. Int Endod J 36:225-231. 2002 https://doi.org/10.1046/j.1365-2591.2003.00652.x
  19. Nair PNR. Duncan HF. Pitt Ford TR. Luder HU. Histological. ultrastructural and quantitative investigations on the response of health human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial. Int Endod J 41:128-150. 2008 https://doi.org/10.1111/j.1365-2591.2007.01329.x
  20. Tziafas D, Kolokuris I, Alvanou A, Kaidoglou K. Shortterm dantinogenic response of dog pulp tissue after its induction by demineralized or native dentine or by predentine. Arch Oral Biol 37:119-128. 1992 https://doi.org/10.1016/0003-9969(92)90007-U
  21. Schroder U. Evaluation of healing following experimental pulpotomy of intact human teeth and capping with calcium hydroxide. OdoIltol Revy 23:329-340, 1972
  22. Heithersay GS. Calcium hydroxide in the treatment of pulpless teeth with associated pathology. J Br Endod Soc 8:74-93, 1975 https://doi.org/10.1111/j.1365-2591.1975.tb01000.x
  23. Siqueil’a JR. Lopez HP. Mechanism of antimicrobial activity of calcium hydroxide: a critical review‘ Int Endod 32:361-369, 1999 https://doi.org/10.1046/j.1365-2591.1999.00275.x
  24. Cox CF, Bergenholtz G, Heys DR, Syed SA, Fitzgerald M, Heys RJ. Pulp-capping of dental pulp mechanically exposed to oral microf1ora: a 1-to-2-year observation of wound healing in the monkey. J Oral Pathol 14:156-168, 1985 https://doi.org/10.1111/j.1600-0714.1985.tb00479.x
  25. Stanley HR, Pameijer CR Dentistry's friend, calcium hydroxide. Oper Dent 22:1-3, 1997 https://doi.org/10.2341/1559-2863-22-4-1
  26. Cox CF, Bergenholtz G, Fitzgerald M, Heys DR. Heys RJ‘ Avery JK, Baker JA, Capping of the dental pulp mechanically exposed to the oral microf1ora: 5-week observation of wound healing in the monkey, J Oral Pathol 11:327-339. 1982 https://doi.org/10.1111/j.1600-0714.1982.tb00173.x
  27. Holland R, De souza V, Nevy NJ, Otobone Filho JA, Bernabo DF, Dozan Jr E. Hoaction of rat connective tissue to implanted dentin tubes filled with mineral trioxide aggrogate or calcium hydoxide , J Endod 25:161-166. 1999 https://doi.org/10.1016/S0099-2399(99)80134-4
  28. TorabinejadM, Watson EF, Pitt Ford TR Sealing abiI. ity. of minoral trioxide aggregate when used as a root end filling material J Endod 19:591-595, 1993 https://doi.org/10.1016/S0099-2399(06)80271-2
  29. Fischer EJ , Arens DE, MillorCR Bacterial leakage of mineral trioxide aggregato as compared with zinc-froo amalgam , intermediate restorative material. and Super-EBA as a root-ond filling material. J Endod 24:176-179, 1998 https://doi.org/10.1016/S0099-2399(98)80178-7
  30. Wu MK. Kontakiotis EG, WesseIink PR. Long-term soal providod by somo root-end fillng materials. J Endod 24:557-560, 1998 https://doi.org/10.1016/S0099-2399(98)80077-0
  31. Torabinejad M, Pitt Ford Tf( McKendry DJ, Abodi HR, Miller DA, Kariyawasam SP. Histological assoss ment of mineral trioxide aggregate as a root-end filling in monkeys. J Endod 23:225-228, 1997 https://doi.org/10.1016/S0099-2399(97)80051-9
  32. Holland R, de Souza V, Nory Mι Otoboni Filho JA, Bernabe PF, Dezan Jr E‘ Reaction of dog’s teeth to root canal filling with mineral trioxide aggregate or a glass ionomer sealer. J Endod 25:728-730, 1999 https://doi.org/10.1016/S0099-2399(99)80118-6
  33. Keiser K. Johnson CC, Tipton DA, Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fiboroblasts. J EIldod 26:288-291. 2000 https://doi.org/10.1097/00004770-200005000-00010
  34. Myers K, Kaminski E, Lautenschalger E, Miller D, The effects of mineral trioxide aggregate on the dog pulp (Abstract), J Endod 22:198, 1996
  35. Song JS , Mante FI\, Romanow WJ, Kim S, Chemical analysis of powder and set forms of Port.1and cement, gray ProRoot MTA, white ProRoot MTA, and gray MTA-Angelus. Oral Surg Oral Med Oral Pathol Oral Radial Endod 102:809-815, 2006 https://doi.org/10.1016/j.tripleo.2005.11.034
  36. Agamy HA, Bakry NS, Mounir MF, Avery DR. Comparison of mineral trioxide aggrega t.e and formocresol as pulp-capping agents in pulpotomized primary teeth. Pediatr Dent 26:302-309, 2004
  37. Maroto M, Barberia E, Vera V, Garcia-Godoy F Dentin bridge formation after white trioxide aggregate (white MTA) pulpotomies in primary molars. Am J Dent 19:75-79, 2006
  38. Parirokh M, Asgary S, Eghb잉 MJ, Stowe S, Eslami B. Eskandarizade A‘ Shabahang S. A comparativo study of white and grey mineral trioxide aggregate as pulp capping agents in dog's teeth. Dent Traumatol 21:150-154, 2005 https://doi.org/10.1111/j.1600-9657.2005.00311.x

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