Journal of Oral Medicine and Pain

  • 제34권1호
  • /
  • Pages.23-37
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  • 2009
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  • 2288-9272(pISSN)
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  • 2383-8493(eISSN)
대한안면통증구강내과학회 (Korean Academy of Orofacial Pain and Oral Medicine)
권호 표지

피톤치드 처리 후 구강 내 잔존 S. thermophilus의 P. gingivalis에 대한 효과

The Effect of S. thermophilus Isolated from Saliva Treated with Phytoncide on P. gingivalis

  • 정성희 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 어규식 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 전양현 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 홍정표 (경희대학교 치의학전문대학원 구강내과학교실)
  • Jung, Sung-Hee (Department of Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Auh, Q-Schick (Department of Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Chun, Yang-Hyun (Department of Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Hong, Jung-Pyo (Department of Oral Medicine, School of Dentistry, Kyung Hee University)
  • 발행 : 2009.03.30
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초록

치주질환과 구취를 유발시키는 중요한 원인균인 P. gingivalis에 대한 피톤치드의 항균효과와 항균작용은 이미 연구되어 있으나, 정상인의 구강상주균에 대한 연구는 아직 희귀한 편이다. 이에 본 연구에서는 건강한 정상인의 타액에 편백 피톤치드를 첨가하였을 때 사멸되지 않고 생존하는 타액세균을 분리하여 구강 유해균과 함께 배양한 후 구강 유해균에 대한 생존 타액세균의 억제효과를 파악함으로써 향후 프로바이오틱으로 작용할 수 있는 구강상주균의 균종을 동정하여 다음과 같은 결론을 얻었다. 1. 정상인의 전타액에 1% 피톤치드를 적용하였을 때 잔존 생균수는 감소하는 경향을 보였다. 2. 피톤치드 적용 후 생존한 주 세균종은 S. thermophilus (53%)로 나타났다. 3. 피톤치드 적용 후 생존한 균을 P. gingivalis A7A1-28과 P. gingivalis W83에 교차배양한 결과 생존균의 대부분(72.5%) 이 P. gingivalis A7A1-28과 P. gingivalis W83의 성장을 억제하였다. 4. 생존 S. thermophilus의 85.8%, S. sanguinis는 75.8%가 P. gingivalis 를 억제하는 것으로 나타났다. 이상의 결과로 미루어, P. gingivalis 등 구강 내 유해균을 직접 억제할 수 있는 것으로 알려진 피톤치드로 처리할 경우 피톤치드에 생존하는 구강상주균이 P. gingivalis에 대해 부가적으로 억제작용을 할 수 있기 때문에 피톤치드의 사용은 치주질환을 예방하고, 그 결과 치주질환 및 구취환자의 구강 환경을 크게 개선할 수 있을 것으로 생각된다.

The antibacterial effect of phytoncide on Porphyromonas gingivalis, which is the main causative agent of periodontal disease and halitosis, has been reported. However, little is known about its effect on normal oral microflora. The present study was performed to observe the effect of phytoncide on oral normal microflora and the inhibitory effect of surviving resident oral bacteria on P. gingivalis. In this study, saliva from each of 20 healthy subjects was treated with 1% phytoncide from Japanese Hinoki (Chamaecyparis obtusa Sieb. et Zucc.). Surviving salivary bacteria were isolated on blood agar plates and identified by 16S rDNA sequencing. In order to select inhibitory isolates against P. gingivalis, the isolates from the phytoncide-treated saliva were cultured with P. gingivalis. The results were as follows: 1. In general, the number of bacteria in saliva from periodontally healthy subjects was decreased when the saliva was treated with 1% phytoncide. 2. The majority of the salivary bacteria surviving the treatment of phytoncide were S. thermophilus (53%). 3. Most of the surviving salivary bacteria (72.5%) inhibit the growth of P. gingivalis A7A1-28 and P. gingivalis W83 on blood agar plates. 4. Among the surviving S. thermophilus, 85.8% of them were observed to inhibit P. gingivalis strains and 75.8% of the surviving S. sanguinis were inhibitory. Taken together, oral resident bacteria surviving phytoncide, which has been shown to inhibit P. gingivalis, may exert an additional inhibitory activity against the periodontopathic bacterium. Therefore, phytoncide can be used for preventing and ceasing the progress of periodontal disease and halitosis, and thus is expect to promote oral health.

키워드

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