The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of Different Extracts of Ostericum koreanum on the Production of Inflammatory Mediators in LPS-stimulated RAW264.7 Cells

강활의 RAW264.7 세포에서 LPS에 의해 유도되는 염증물질 생성에 대한 효과

  • Kim, Chang-Min (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
  • 김창민 (동국대학교 한의과대학 본초학교실) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Published : 2009.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : In this study, the effects of ethylacetate extract of Ostericum koreanum on inflammation in RAW264.7 cells were investigated. Methods : Dried roots of Ostericum koreanum was extracted with 80% methanol for 24 h, and then fractionated with n-butanol, n-hexan and ethylacetate. RAW264.7 cells, a mouse macrophage line were incubated with different concentrations of the extract for 30 min and then stimulated with LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of nitric oxide (NO) and prostaglandin E2 ($PGE_2$) were measured by Griess assay and enzyme immunoassay (EIA), respectively. The expressions of inducible nitric oxide synthease (iNOS) and cyclooxyganase (COX) -2 mRNA and protein were determined by RT-PCR and Western blot. Results : The methanol extract of Ostericum koreanuman and its fractions were significantly inhibited the NO and PGE2 productions in LPS-stimulated RAW264.7 cells. Among the fractions of Ostericum koreanuman the ethylacetate fraction was more strongly inhibited NO and $PGE_2$ productions compared with other fractions. The ethylacetate fraction was also suppressed LPS-induced mRNA expressions of iNOS and its protein levels in RAW264.7 cells. Conclusions : This study suggests that the ethylacetate fraction of Ostericum koreanum may have an anti-inflammatory property through suppressing inflammatory mediator productions in activated macrophages, suggesting have a therapeutic potential for the treatment of various inflammatory diseases.

Keywords

References

  1. Morson BC. Pathology of inflammatory bowel disease. Gastroenterol Jpn. 1980 ; 15(2) : 184-7.
  2. Cline MJ. Leukocyte function in inflammation: the ingestion, killing, and digestion of microorganisms. Ser Haematol. 1970 ; 3(2) : 3-16.
  3. Guo LY, Hung TM, Bae KH, Shin EM, Zhou HY, Hong YN, Kang SS, Kim HP, Kim YS. Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill. Eur J Pharmacol. 2008 ; 591(1-3) : 293-9. https://doi.org/10.1016/j.ejphar.2008.06.074
  4. Bosca L, Zeini M, Traves PG, Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005 ; 208(2) : 249-58 https://doi.org/10.1016/j.tox.2004.11.035
  5. Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992 ; 6(12) : 3051-64.
  6. Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annu Rev Med. 2002 ; 53 : 35-57. https://doi.org/10.1146/annurev.med.53.082901.103952
  7. Wei W, Li XY, Zhang HQ, Wu SG. Anti-inflammatory and immuno pharma cology. 1st ed. Beijing : Renmin weisheng chubanshe. 2004. 10-17.
  8. Andreakos E, Foxwell B, Feldmann M. Is targeting Toll-like receptors and their signaling pathway a useful therapeutic approach to modulating cytokine-driven inflammation. Immunol Rev. 2004 ; 202 : 250-65. https://doi.org/10.1111/j.0105-2896.2004.00202.x
  9. Cao H, Urban JF Jr, Anderson RA. Cinnamon polyphenol extract affects immune responses by regulating anti- and pro inflammatory and glucose transporter gene expression in mouse macrophages. J Nutr. 2008 ; 138(5) : 833-40.
  10. Tao JY, Zhao L, Huang ZJ, Zhang XY, Zhang SL, Zhang QG, Fei-Xiao, Zhang BH, Feng QL, Zheng GH. Anti-inflammatory effects of ethanol extract from Kummerowia striata (Thunb.) Schindl on lps-stimulated RAW 264.7 cell. Inflammation. 2008 ; 31(3) : 154-66. https://doi.org/10.1007/s10753-008-9061-7
  11. Zhao L, Zhang SL, Tao JY, Jin F, Pang R, Guo YJ, Ye P, Dong JH, Zheng GH. Anti- inflammatory mechanism of a folk herbal medicine, Duchesnea indica (Andr) Focke at RAW264.7 cell line. Immunol Invest. 2008 ; 37(4) : 339-57. https://doi.org/10.1080/08820130802111589
  12. Jung HW, Yoon CH, Kim YH, Boo YC, Park KM, Park YK. Wen-Pi-Tang-Hab-Wu- Ling-San extract inhibits the release of inflammatory mediators from LPS-stimulated mouse macrophages. J Ethnopharmacol. 2007 ; 114(3) : 439-45. https://doi.org/10.1016/j.jep.2007.08.035
  13. Rhule A, Navarro S, Smith JR, Shepherd DM. Panax notoginseng attenuates LPS -induced pro-inflammatory mediators in RAW264.7 cells. J Ethnopharmacol. 2006 ; 106(1) : 121-8. https://doi.org/10.1016/j.jep.2005.12.012
  14. Jin HZ, Lee JH, Lee D, Hong YS, Kim YH, Lee JJ. Inhibitors of the LPS-induced NF-kappaB activation from Artemisia sylvatica. Phytochemistry. 2004 ; 65(15) : 2247-53. https://doi.org/10.1016/j.phytochem.2004.06.034
  15. 全國韓醫科大學 共同敎材編輯委員會 編著. 本草學. 永林社. 2007 : 159-61.
  16. 馬繼興. 神農本草經輯注. 北京 : 人民衛生出版社. 1995 : 64-5
  17. 中國醫學科學院 藥物硏究所. 中藥志(第2冊). 北京 : 人民衛生出版社. 1975 : 397-401.
  18. 許浚. 東醫寶鑑. 서울 : 대성문화사. 1986 : 721-2.
  19. 한국약학대학협의회 약전분과회. 대한약전 제8개정 해설서. 서울 : 신일상사. 2003 : 141.
  20. 全國中草藥匯編寫組. 全局中草藥匯編. 北京 : 人民衛生出版社. 1983 : 301.
  21. 中國醫學科學院 藥物硏究所. 中藥志(第2冊). 北京 : 人民衛生出版社. 1975 : 397-401.
  22. 國家藥典委員會. 中華人民共和國藥典(一部). 北京 : 化學工業出版社. 2000 : 144-5.
  23. 雷載權, 張廷模. 中華臨床中藥學(上卷). 北京: 人民衛生出版社. 1998 : 204-9
  24. Shin S. In vitro effects of essential oils from Ostericum koreanum against antibiotic-resistant Salmonella spp. Arch Pharm Res. 2005 ; 28(7) : 765-9. https://doi.org/10.1007/BF02977340
  25. Zschocke S, Lehner M, Bauer R. 5- Lipoxygenase and cyclooxygenase inhibitory active constituents from Qianghuo (Notop terygium incisum). Planta Med. 1997 ; 63(3) : 203-6. https://doi.org/10.1055/s-2006-957653
  26. Rocca B, FitzGerald GA. Cyclooxygenases and prostaglandins: shaping up the immune response. Int Immunopharmacol. 2002 ; 2(5) : 603-30. https://doi.org/10.1016/S1567-5769(01)00204-1
  27. Lowenstein CJ, Snyder SH. Nitric oxide, a novel biologic messenger. Cell. 1992 ; 70(5) : 705-7. https://doi.org/10.1016/0092-8674(92)90301-R
  28. Sunyer T, Rothe L, Jiang X, Anderson F, Osdoby P, Collin-Osdoby P. Ca2+ or phorbol ester but not inflammatory stimuli elevate inducible nitric oxide synthase messenger ribonucleic acid and nitric oxide (NO) release in avian osteoclasts: autocrine NO mediates Ca2+-inhibited bone resorption. Endocrinology. 1997 ; 138(5) : 2148-62. https://doi.org/10.1210/en.138.5.2148
  29. Park JY, Pillinger MH, Abramson SB. Prostaglandin $E_2$ synthesis and secretion: the role of $PGE_2$ synthases. Clin Immunol. 2006 ; 119(3) : 229-40. https://doi.org/10.1016/j.clim.2006.01.016