The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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A Therapeutic Effect of Pinellia Ternata via the Increase of CD4+CD25+ Regulatory T Cells and the Suppression of CD3+CCR3+ Cellular Infiltration During Allergic Airway Inflammation

천식 모델 생쥐에서 반하(半夏)의 CD4+CD25+ 조절 T 세포 상승 및 CD3+CCR3+Th2 세포 침윤 억제 효과

  • Lee, Young-Cheol (Department of Herbology, College of Oriental Medicine, Sangji University)
  • 이영철 (상지대학교 한의과대학 본초학교실)
  • Published : 2009.03.30
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Abstract

Objectives : In this study, we studied the effect of Pinellia Ternata (PT) on regulatory T cells and CD3+CCR3+ Th2 cells number in asthma model mice. Methods : All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2 $m\ell$ alum-precipitated Ag containing 100 ${\mu}g$ of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min, 2.5％ ovalbumin in normal saline) and PT (400, 200 mg/kg) were orally administered 3 times a week for 8 weeks. After C57BL/6 mice were orally given of PT, the percentages, cell numbers, phenotype and function of CD4+CD25+Treg cells were determined by flow cytometry. Results : The cell numbers of CD4+CD25+Treg cell subsets were markedly increased in PT treated mice as reported. However, PT significantly reduced the CD3+CCR3+ Th2 cells in PBMC and lung of mice. Conclusions : These results indicate that PT has a deep inhibitory effect on asthma model mice by increase the number of regulatory T cells, and by reducing CD3+CCR3+ Th2 cells.

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References

  1. Busse WW, Lemanske RF. Jr Asthma. N Engl J Med. 2001 ; 344 : 350-62. https://doi.org/10.1056/NEJM200102013440507
  2. Sakaguchi S. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and non-self. Nat Immunol. 2005 ; 6 : 345. https://doi.org/10.1038/ni1178
  3. Hawrylowicz CM, O'Garra A. Potential role of interleukin-10-secreting regulatory T cells in allergy and asthma. Nat Rev Immunol. 2005 ; 5 : 1271-83.
  4. Akdis CA, Blesken T, Akdis M, Wuthrich B, Blaser K. Role of interleukin 10 in specific immunotherapy. J Clin Invest. 1998 ; 102 : 98-106. https://doi.org/10.1172/JCI2250
  5. Marki T, Takahashi K, Shibata S. An antiemetic principle of Pinellia ternate. Planta Med. 1987 ; 53 : 410-4. https://doi.org/10.1055/s-2006-962759
  6. Zhao L. Comparative analysis of alkaloid components in cultured and planted Pinellia ternata (Thunb.). Breit China J Chin Med. 1990 ; 15 : 146-7, 189-90.
  7. Wang R, Ni JM, Ma R. Volatile oils of Pinellia ternate. J Chin Pharm. 1995 ; 30 : 457-9.
  8. Tomoda M, Gonda R, Ohara N, Shimizu N, Shishido C, Fujiki Y. A glucan having reticuloendothelial system-potentiating and anti-complementary activities from the tuber of Pinellia ternate. Bio Pharm Bull. 1994 ; 17 : 859-61. https://doi.org/10.1248/bpb.17.859
  9. Kroegel C, Liu MC, Hubbard WC, Lichtenstein LM, Bochner BS. Blood and bronchoalveolar eosinophils in allergic subjects after segmental antigen challenge: surface phenotype, density heterogeneity, and prostanoid production. J Allergy Clin Immunol. 1994 ; 93 : 725. https://doi.org/10.1016/0091-6749(94)90252-6
  10. Wardlaw AJ, Dunnette S, Gleich GJ, Collins JV, Kay AB. Eosinophils and mast cells in bronchoalveolar lavage in subjects with mild asthma: relationship to bronchial hyperreactivity. Am Rev Respir Dis. 1988 ; 137 : 62. https://doi.org/10.1164/ajrccm/137.1.62
  11. Thornton AM, Shevach EM. Suppressor effector function of CD25+CD4+ immunoregulatory T cells is antigen nonspecific. J Immunol. 2000 ; 164 : 183-90. https://doi.org/10.4049/jimmunol.164.1.183
  12. Asseman C, Mauze S, Leach MW, Coffman RL, Powrie F. An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. J Exp Med. 1999 ; 190 : 995-1004. https://doi.org/10.1084/jem.190.7.995
  13. Powrie F, Carlino J, Leach MW, Mauze S, Coffman RL. A critical role for transforming growth factor b but not interleukin 4 in the suppression of T helper type 1-mediated colitis by CD45RBlow CD4+ T cells. J Exp Med. 1996 ; 183 : 2669-74. https://doi.org/10.1084/jem.183.6.2669
  14. Kay AB. Allergy and allergic diseases. N Engl J Med. 2001 ; 344 : 30-7. https://doi.org/10.1056/NEJM200101043440106
  15. Dunnill MS. The pathology of asthma, with special reference to changes in the bronchial mucosa. J Clin Pathol. 1960 ; 13 : 27-33. https://doi.org/10.1136/jcp.13.1.27
  16. Duvernelle C, Freund V, Frossard N. Transforming growth factor-beta and its role in asthma. Pulm Pharmacol Ther. 2003 ; 16 : 181-96. https://doi.org/10.1016/S1094-5539(03)00051-8
  17. Beijing University of Traditional chinese medicine. The chinese materia medica. Beijing. China : Academy Press [Xue Yuan]. 1998 : 212-5.
  18. Lukacs NW. Role of chemokines in the pathogenesis of asthma. Nature Immunology Rev. 2001 ; 1 : 108-16. https://doi.org/10.1038/35100503
  19. Powell N, Humbert M, Durham SR, Assoufi B, Kay AB, Corrigan CJ. Increased expression of mRNA encoding RANTES and MCP-3 in the bronchial mucosa in atopic asthma. Eur Respir J. 1996 ; 9 : 2454-60. https://doi.org/10.1183/09031936.96.09122454