Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Effect of Chestnut on lipid Metabolism and Antithrombotic Capacity in Rats

랫드에서 밤의 과육 및 내피가 지방대사 및 항혈전능에 미치는 영향

  • Kim, So-Jung (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Mi-Hwan (Dept. of Food Science and Technology, Kongju National University) ;
  • Kim, Jin (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Hyeong-Jin (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Soo-Hyun (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Seung-Ho (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Young-Seok (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Byung-Kwon (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Byeong-Soo (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Sang-Ki (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Choi, Chang-Sun (Dept. of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Ryu, Gi-Hyung (Dept. of Food Science and Technology, Kongju National University) ;
  • Jung, Ji-Youn (Dept. of Companion and Laboratory Animal Science, Kongju National University)
  • 김소정 (공주대학교 특수동물학과) ;
  • 김미환 (공주대학교 식품공학과) ;
  • 김진 (공주대학교 특수동물학과) ;
  • 김형진 (공주대학교 특수동물학과) ;
  • 김수현 (공주대학교 특수동물학과) ;
  • 이성호 (공주대학교 특수동물학과) ;
  • 박영석 (공주대학교 특수동물학과) ;
  • 박병권 (공주대학교 특수동물학과) ;
  • 김병수 (공주대학교 특수동물학과) ;
  • 김상기 (공주대학교 특수동물학과) ;
  • 최창순 (중앙대학교 식품영양학과) ;
  • 류기형 (공주대학교 식품공학과) ;
  • 정지윤 (공주대학교 특수동물학과)
  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the effect of dried powder of chestnut on lipid metabolism, anti-thrombotic effect in rats. Thirty 5-week-old male Sprague Dawley (SD) rats were randomly allocated into five groups and used for experiment. We examined the lipid metabolism and antithrombotic capacity of SD rats administered for 5 weeks with 0.16 g/kg, 0.5 g/kg chestnut flesh powder and 0.16 g/kg, 0.5 g/kg chestnut inner shell and flesh powder mixture, respectively. Food intake, body weight gain and food efficiency ratio were also checked. The levels of serum triglyceride and tree fatty acid were not statistically significant between the all experimental groups. However, the antithrombotic capacity and total lipid levels of the treatment groups were significantly lower than those of the negative control group. These results suggest that the supplementation of chestnut on diet lower the total lipid level in SD rats.

본 연구는 농산물 수입개방으로 우리농산물의 고부가가치 창출에 힘써야 하는 이때에 비만인구의 증가과 이에 따른 순환기계질환의 심각성을 인식하고 예부터 기호식품이나 대용식량으로 널리 이용되어 왔던 밤을 이용하여 기능성식품으로서의 가치를 알아보고자 하였다. 실험은 일주일에 5번씩 총 5주 동안 5주령의 수컷 SD rat를 대상으로 밤시료를 경구투여하였다. 실험군은 음성대조군(non chestnut group), 과육 저용량 투여군(0.16 g/kg), 과육 고용량 투여군(0.5 g/kg), 과육내피 저용량 투여군 (0.16 g/kg), 과육내피 고용량 투여군(0.5 g/kg)으로 구분하여 지질대사와 항혈전능에 미치는 영향을 비교 분석하였다. 실험결과 랫드의 체중증가량과 상대장기중량에서 통계학적으로 유의적인 변화가 관찰되지 않았지만, 사료섭취량과 체중증가량을 이용하여 각 군의 5주간 식이효율을 구해보았을 때 과육 저용량군의 식이효율이 대조군의 식이효율보다 유의적으로 감소하였다. 혈청 중 triglyceride, free fatty acid의 수치는 과육내피 고용량군을 제외하고 대조군에 비하여 감소하는 경향을 나타내었으며, total lipid의 수치는 모든 투여군이 대조군에 비하여 감소하였고 특히 과육 저용량군, 과육 고용량군, 과육내피 저용량군에서 수치가 유의적으로 감소하였다. 한편, glucose의 수치는 대조군에 비해 과육 고용량군, 과육내피 고용량군에서 유의적인 증가를 관찰할 수 있었다. 혈청 total cholesterol과 HDL-cholesterol의 수치를 이용한 Atherogenic index 즉, 동맥경화지수는 대조군과 비교하여 봤을 때 모든 투여 군에서 증가하는 경향을 보였지만 특히 과육 고용량군과 과육내피 고용량군의 수치가 과육 저용량군과 과육내피 저 용량군의 수치보다 높은 것으로 나타났다. 이상의 결과를 종합해 봤을 때 내피를 함유한 식이보다 과육만을 함유한 식이가 랫드에서 지질대사와 항혈전능에 긍정적인 영향을 미치는 것으로 나타났다. 따라서 우리나라에서 대량으로 생산되고 있는 밤나무 열매의 과육이 기능성 건강식품의 소재로 이용될 수 있는 적절한 물질이라 판단된다.

Keywords

References

  1. 이지연, 박경애: 2006년 사망 및 사망원인통계결과. 통계청 인구동향과 (2007)
  2. 임업연구원: 밤나무 재배관리 기술. 16 (2001)
  3. 김정호, 김종천, 고광출, 김규래, 이재창: 과수원예각론. 항문사, 서울, 404-420(1999)
  4. Oh, S.H., Kim, YH. and Lee, S.N.: Purification and properties of the peroxidase in castanea semen. Kor. J. Food Sci. Technol, 19, 506-514 (1987)
  5. Kim, S.K., Jeon, YJ., Kim, Y.T., Lee, B.J. and Kang, O.J.: Physicochemical and textural properties of chestnut starches. J. Korean Soc. Food Nutr, 24, 594-600 (1995)
  6. Yim, H., Kim, C.O., Shin, D.W. and Sub, K.B.: Study on the storage of chestnut. Korean J. Food Sci. Technol, 12, 170-175 (1980)
  7. Na, YA. and Yang, C.B.: Changes of lipids in chestnut during storage. Korean J. Food Sci. Technol, 29, 437-445 (1997)
  8. Cho, GS. and Kim, H.Y.: Screening of Antimicrobial Activity from Castanea crenata Sieb. et ZUCCo Leaves and Flowers. J. Korean Soc. Appl. Bioi. Chem, 46, 262-267 (2003)
  9. Gerardina, A., Luciano, C., Donatella, A. and Marisa, D.M.: Effects of drying temperatures on physico-chemical properties of dried and rehydrated chestnuts (Castanea sativa). Food Chemistry, 88, 583-590 (2004) https://doi.org/10.1016/j.foodchem.2004.01.071
  10. Miguez, B.M., Montafia, MJ. and Garcia, Q.J.: HPLC determination of sugars in varieties of chestnut fruits from galicia. J. Food Compos. Anal, 17,63-67 (2004) https://doi.org/10.1016/S0889-1575(03)00093-0
  11. Bylik, A. and Sapers, GM.: Distribution of quercetin and kaempferol in lettuce, kale, chive, garlic chive, leek, horseradish, red radish, and red cabbage tissues. J. Agric. Food Chem, 33, 226-228 (1985) https://doi.org/10.1021/jf00062a017
  12. Hertog, M.GL., Hollman, P.C.H. and Katan, M.B.: Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J. Agric. Food Chem, 40, 2379-2383 (1992) https://doi.org/10.1021/jf00024a011
  13. Pratt, D.E. and Hudson, B.J.F.: In food antioxidants: Natural antioxidants not exploited commercially. Elsevier Applied Science, London, UK, 171-192 (1990)
  14. Anila, L. and vijayalakshmi, N.R.: Flavonoids from emblica officinalis and mangifera indica-effectiveness for dyslipidemia. J. Ethnopharmacol, 79, 81-87 (2002) https://doi.org/10.1016/S0378-8741(01)00361-0
  15. Na, YA. and Yang, C.B.: Changes of constituent components in chestnut during storage. Korean J. Food Sci. Technol, 28, 1164-1170 (1996)
  16. Vasconcelos, M.C., Bennett, R.N., Rosa, E.A. and Cardoso, J.V.F.: Primary and secondary metabolite composition of kernels from three cultivars of Portuguese chestnut (Castanea sativa Mill.) at different stages of industrial transformation. J. Agric. Food. Chem, 55, 3508-3516 (2007) https://doi.org/10.1021/jf0629080
  17. Kwon, E.J., Kim, YC., Kwon, M.S., Kim, C.S., Kang, W.W., Lee, J.B. and Chung, S.K.: Antioxidative activity of solvent fraction and isolation of antioxidant compound from chestnut husk. J. Korean Soc. Food Sci. Nutr, 30,726-731 (2001)
  18. Vekiari, S.A., Gordon, M.H., Garcia, M.P. and Labrinea, H.: Extraction and determination of ellagic acid content in chestnut bark and fruit. Food Chem, 110, 1007-1011 (2008) https://doi.org/10.1016/j.foodchem.2008.02.005
  19. Hornstra,G and Kester, A.D.: Effect of the dietary fat type on arterial thrombosis tendency. systematic studies with a rat model. Atherosclerosis, 131, 25-33 (1997) https://doi.org/10.1016/S0021-9150(97)06094-2
  20. Yamazaki, K. and Murata, M.: Frequency of atherogenic risk factors in Japanese obese children. Diabetes. Res. Clin. Pract, 10, S211-S219 (1990) https://doi.org/10.1016/0168-8227(90)90166-Q
  21. 정순동: 가축과 실험동물의 생리자료. 광일문화사, 서울, 694 (1996)
  22. Ikeda, I., Imasato, Y, Sasaki, E., Nakayama, M., Nagao, H., Takeo, T., Yayabe, F. and Sugano, M.: Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. Biochim. Biophys. Acta, 1127, 141-146 (1992) https://doi.org/10.1016/0005-2760(92)90269-2
  23. Waterhouse, A.L.: Wine and heart disease. Chemistry and industry, 5, 338-341 (1995)
  24. Lee, J.I., Lee, H.S., Jun, W.J., Yu, K.W., Shin, D.H., Hong, B.S., Cho, H.Y and Yang, H.C.: Anticoagulation activities pattern and in vivo test of extract from eugenia caryophyllata. J. Korean Soc. Food Sci. Nutr, 29, 543-548 (2000)
  25. Petroni, A., Biasevich, M., Salami, M., Papini, N., Montedoro, GF. and Galli, C.: Inhibition of platelet aggregation and eicosanoid production by phenolic components of olive oil. Thromb. Res, 78, 151-160 (1995) https://doi.org/10.1016/0049-3848(95)00043-7
  26. Laughton, M.J., Evans, P.J., Moroney, M.A., Houit, J.R. and Halliwell, B.: Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives: Relationship to antioxidant activity and to iron ionreducing ability. Biochem. Pharmacol, 42, 1673-1681 (1991) https://doi.org/10.1016/0006-2952(91)90501-U
  27. Viana, M., Barbas, C., Bonet, B., Bonet, M.V., Castro, M., Fraile, M.V. and Herrera, E.: In vitro effects of a flavonoidrich extract on LDL oxidation. Atherosclerosis, 123, 83-91 (1996) https://doi.org/10.1016/0021-9150(95)05763-3
  28. Hertog, M.G, Feskens, E.J., Hollman, P.C., Katan, M.B. and Kromhout, D.: Dietary antioxidant flavonoids and risk of coronary heart diease: The Zutphen Elderly Study. Lancet, 342, 1007-1011 (1993) https://doi.org/10.1016/0140-6736(93)92876-U
  29. Jung, U.J., Lee, M.K., Park, YB., Kang, M.A. and Choi, M.S.: Effects of citrus flavonoids on lipid metabolism and glucose-regulating enzyme mRNA level in type-2 diabetic mice. Int. J. Biochem. Cell BioI. 38, 1134-1145 (2006) https://doi.org/10.1016/j.biocel.2005.12.002
  30. Glomset, J.A.: Physiological role of lecithin-cholesterol acyltransferase. Am. J. Clin. Nutr, 23, 1129-1136 (1970)
  31. Miller, G.J. and Miller, N.E.: Plasma-high-density-lipoprotein concentration and development of ischaemic heart-disease. Lancet, 1, 16-19 (1975) https://doi.org/10.1016/S0140-6736(02)95332-8