Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Desmutagenic Effect of Water Extract from Cassia tora L. on the Mutagenicity of N-methyl-N-nitro-N'-nitrosoguanidinein in E. coli PQ37

N-methyl-N-nitro-N'-nitrosoguanidine의 변이원성에 대한 결명자 물 추출물의 항돌연변이 효과

  • Ahn, Byung-Yong (Department of Oriental Medicine Resources, Chonbuk National University)
  • Published : 2009.03.31
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Abstract

The desmutagenic activity of the water extract of Cassia tara L on the mutagenicity induced by 4-nitroquinoline 1-oxide (4-NQO) and N-methyl-N-nitro-N'-nitrosoguani-dine (MNNG) was studied using the SOS Chromotest with Escherichia coli PQ37. The inhibition rates of water extract of Cassia tara L. at concentration of $100{\mu}g$/assay were 27.5% and 40% against 4-NQO and MNNG. The water extract of Cassia tara L. was separated into methanol soluble and methanol insoluble parts. The methanol soluble part exhibited higher inhibition effects than the methanol insoluble part against the mutagenic activities of 4-NQO and MNNG. Step-wise fractionation of methanol soluble part was done to obtain methanol, ethyl acetate and water fractions. Among these fractions, water fraction had the strongest inhibitory effects of 23.0 and 19.0% against mutagenicities of MNNG and 4NQO, respectively. The results clearly indicated that the water fraction showed much stronger antimutagenicity against MNNG than 4NQO. The inhibition rates of aqueous fraction of methanol-soluble from water extracted Cassia tara L. at concentrations of 1.0, 10, 100 and $250{\mu}g$/assay were 8.0%, 12.0%, 25.5% and 43.0%, respectively. The water fraction showed the inhibitory effects with dose response against the mutagenic activities induced by MNNG.

4-nitroquinoline 1-oxide (4-NQO)와 N-methyl-N-nitro-N'-nitrosoguanidinein (MNNG)에 의해 유도된 돌연변이원성에 대한 결명자 물 추출물의 억제효과를 E. coli PQ37 이용한 SOS Chromotest법으로 검색하였다. $100{\mu}g$/assay 농도에서 4-NQO와 MNNG의 변이원성에 대한 결명자 물 추출물의 변이원성 억제 효과는 27.5 및 40%로 나타났다. 결명자 물 추출물을 메탄을 가용성 부분과 메탄을 불용성 부분으로 분리하여 4-NQO와 MNNG의 변이원성에 대한 억제효과를 검색한 결과 메탄을 가용성 부분이 더 강한 효과를 나타내었다. 따라서 결명자 메탄을 가용성 부분을 에틸아세테이트, 부탄을 그리고 물로 각각 분획하여, 4-NQO 및 MNNG에 대한 돌연변이 억제효과를 검색한 결과 물 분획물은 각각 19.0, 및 23.0% 억제효과로 가장 강하게 나타내었으며, MNNG의 변이원성에 대한 억제효과가 4-NQO의 변이원성에 대한 억제효과보다 더 강하게 나타남을 확인하였다. 결명자 열수추출물의 메탄을 가용성부분의 물 분획물의 농도를 assay당 1.0, 10, 100 및 $250{\mu}g$으로 증가시켰을 경우, 항돌연변이 효과는 각각 8.0%, 12.0%, 25.5%및 43.0%로 나타났다. 이러한 결과로부터, 결명자 물 분획물은 MNNG의 변이원성에 대하여 용량 의존적 억제효과를 나타냄을 확인하였다.

Keywords

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