Journal of Periodontal and Implant Science

  • 제39권1호
  • /
  • Pages.45-52
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  • 2009
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

피브린 결합능을 가진 합성 펩타이드의 치주인대 세포에 대한 생물학적 영향

The biologic effect of fibrin-binding synthetic oligopeptide on periodontal ligament cells

  • 이주아 (서울대학교 치의학대학원 치주과학교실) ;
  • 구영 (서울대학교 치의학대학원 치주과학교실) ;
  • 박윤정 (서울대학교 치의학대학원 두개악안면재건과학교실) ;
  • 구기태 (서울대학교 치의학대학원 치주과학교실) ;
  • 김태일 (서울대학교 치의학대학원 치주과학교실) ;
  • 설양조 (서울대학교 치의학대학원 치주과학교실) ;
  • 이용무 (서울대학교 치의학대학원 치주과학교실) ;
  • 류인철 (서울대학교 치의학대학원 치주과학교실) ;
  • 정종평 (서울대학교 치의학대학원 치주과학교실)
  • Lee, Ju-A (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Park, Yoon-Jeong (Craniomaxillofacial Reconstructive Science Major, School of Dentistry, Seoul National University) ;
  • Koo, Ki-Tae (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Kim, Tae-Il (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Seol, Yang-Jo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, School of Dentistry, Seoul National University)
  • 발행 : 2009.03.31
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초록

Purpose: Fibronectin(FN), one of the major components of ECM, mediates wide variety of cellular interactions including cell adhesion, migration, proliferation and differentiation. In this study, we used synthetic peptides based on fibrin binding sites of amino-terminal of FN and evaluated their biologic effects on periodontal ligament(PDL) cells. Materials and methods: PDL cells were cultured on synthetic oligopeptides coated dishes and examined for cell adhesion, proliferation via confocal microscope. For detection of ERK1/2, cells were plated and Western blot analysis was performed. Results: PDL cells on synthetic oligopeptide coated dishes showed enhanced cell adhesion and proliferation. Western blot analysis revealed increased level of ERK1/2 phosphorylation in cells plated on FN fragment containing fibrin-binding domain(FF1 and FF5) coated dishes. Conclusion: These results reveals that FN fragment containing fibrin-binding domain possess an enhanced biologic effect of PDL ligament cells.

키워드

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