Phenolic Compounds Content and DPPH, ADH, ALDH Activities of Mungbean Sprout Based on Growth Temperature

녹두나물 재배온도에 따른 페놀화합물 함량과 DPPH, ADH 및 ALDH 활성

This study is to analyze the effects of the growth temperature of mungbean sprouts ($15{\sim}30{\pm}1^{\circ}C$) on the yield ratio, content of phenolic compounds and DPPH (1,1-diphenyl-2-picrylhydrazyl), ADH (alcohol dehydrogenase), ALDH (aldehyde dehydrogenase) activities of the sprouts. When the growth temperature of mungbean sprouts was higher, the yield ratio of the sprouts was higher while the hard seed rate was lower, but $25{\pm}1^{\circ}C$ and $30{\pm}1^{\circ}C$ showed no regular tendency. The content of the total phenol from the ethanol extract of the sprouts was higher in the growth temperature of $15{\pm}1$, $20{\pm}1$, and $25{\pm}1^{\circ}C$, while the content of total flavonoid was higher in the growth temperature of $15{\pm}1$, and $20{\pm}1^{\circ}C$. The DPPH radical scavenging activity of the ethanol extract of the sprouts was higher when the growth temperature was lower, while the activity of ADH and ALDH showed no regular tendency according to the growth temperature. Considering the yield ratio, content of phenolic compounds, biological activities of mungbean sprouts, the optimum cultivation temperature of mungbean sprouts may be $20{\sim}25^{\circ}C$.

본 연구는 녹두나물 재배온도($15{\sim}30{\pm}1^{\circ}C$)에 따른 나물의 생육과 수율, 페놀화합물 함량, DPPH와 ADH 및 ALDH활성에 미치는 영향을 분석하였다. 1. 녹두나물 재배온도가 높을수록 나물 생산수율은 높고, 경실비율은 낮았으나 $25{\pm}1^{\circ}C$와 $30{\pm}1^{\circ}C$는 큰 차이가 없었다. 2. 녹두나물의 ethanol 추출물의 총페놀 함량은 재배온도 $15{\pm}1$, $20{\pm}1$, $25{\pm}1^{\circ}C$, 총플라보노이드 함량은 재배온도 $15{\pm}1$, $20{\pm}1^{\circ}C$에서 많았다. 3. 녹두나물 ethanol 추출물의 DPPH 라디칼 소거활성은 재배온도가 낮을수록 높았고, ADH와 ALDH활성은 재배온도 간 일정한 경향을 보이지 않았다. 4. 이상의 녹두나물 생산수율, 페놀화합물 함량, 생리활성 등을 고려해볼 때 녹두나물 재배 최적온도는 $20{\sim}25^{\circ}C$로 보아진다.