Journal of Mushroom (한국버섯학회지)
- Volume 7 Issue 3
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- Pages.98-104
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- 2009
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- 1738-0294(pISSN)
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- 2288-8853(eISSN)
Phylogenetic relationships of Lyophyllum decastes on the based of ITS region sequences and RAPD
잿빛만가닥버섯(Lyophyllum decastes)의 ITS 영역염기서열 및 RAPD에 의한 계통학적 유연관계 분석
- Woo, Sung-Mi (The Korean Society of Spawn Production) ;
- Park, Yong-Hwan (The Korean Society of Spawn Production) ;
- Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
- Shin, Pyung-Gyun (The Korean Society of Spawn Production) ;
- Jang, Kab Yeul (The Korean Society of Spawn Production) ;
- Jin, Yong-Ju (The Korean Society of Spawn Production) ;
- Sung, Jae-Mo (Department of Applied Biology Graduate School, Kangwon National University)
- 우성미 (한국종균생산협회) ;
- 박용환 (한국종균생산협회) ;
- 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
- 신평균 (한국종균생산협회) ;
- 장갑열 (한국종균생산협회) ;
- 진용주 (한국종균생산협회) ;
- 성재모 (강원대 응용생물학과)
- Received : 2009.04.22
- Accepted : 2009.09.05
- Published : 2009.09.30
Abstract
Phylogenetic relationships of Hypsizygus mamoreus and Lyophyllum decastes artificial cultivated using ITS sequences and RAPD polymorphism have been analyzed. Based on ITS region sequences of 14 strains, we could divide into 2 group as group1 to Hypsizygus mamoreus and the control isolated group2 to Lyophyllum decastes were identified as the same species. Restrict analysis of rDNA ITS region which was amplified by PCR, 14 collected strains could be classified into 4 clusters. There was approximately 58% genetic similarity between cluster I(SPA 100, 101, 102) and cluster II(SPA 200, 208 and SPA 201, 202), 41% between cluster III(SPA 104, 103, 203) and cluster IV(SPA 204, 206, 207, 205) by BLAST analysis. RAPD polymorphisms were used to analyze the species diversity of artificially cultivated Lyophyllum decastes SPA 202. As a result, similarity between SPA 202 and SPA 203 was 75%, at the same time, similarity between SPA 202 and Pleurotus eryngii SPA 103 and SPA 104 was 65%.
인공재배된 만가닥버섯(Hypsizygus mamoreus)과 잿빛 만가닥버섯(Lyophyllum decastes)을 ITS