Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Quinone Reductase Inductive Activity and Growth Inhibitory Effect against Hepatoma Cell of Oriental Melon Extract

참외 추출물의 Quinone Reductase 유도활성 및 간암세포 증식 억제효과

  • Kim, Hye-Suk (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University) ;
  • Ku, Kang-Mo (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University) ;
  • Suh, Jun-Kyu (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University) ;
  • Kang, Young-Hwa (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University)
  • 김혜숙 (경북대학교 농업생명과학대학 원예학과) ;
  • 구강모 (경북대학교 농업생명과학대학 원예학과) ;
  • 서전규 (경북대학교 농업생명과학대학 원예학과) ;
  • 강영화 (경북대학교 농업생명과학대학 원예학과)
  • Published : 2009.12.31
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Abstract

This study was performed to elucidate anticancer activities of various parts, such as peel, flesh, placenta, seed, stalk and stem leaf of oriental melon. Chemopreventive and anticancer effects of oriental melon extract were evaluated by detoxifying enzyme, quinone reductase (QR) inductive activity, cytotoxicity and growth inhibitory effect against hepatoma cell. Stalk and stem leaf extracts of oriental melon showed the increment of QR inductive activity with dose-dependent manner and induced quinone reductase 3.9, 1.5-fold at $200{\mu}g/mL$ respectively compared to control. The growth inhibitory effect of oriental melon extract against mouse hepatoma cell (Hepa1c1c7) was investigated by crystal violet (CV) assay. Stalk and stem leaf of oriental melon showed potent growth inhibitory effect. Based on these result, the growth inhibitory effects of stalk, stem leaf at various concentration were examined in detail by MTT assay using human hepatoma cancer cell (HepG2). All of two parts showed growth inhibitory effects and expecially stalk exhibited inhibitory effect of 60.3% at maximum concentration. The above results suggest that stalk of oriental melon has a possibility as a source of natural cancer chemopreventive materials.

본 연구에서는 참외 추출물의 항암활성에 대해 알아보기 위해 참외를 부위별로 나누어 quinone reductase 유도활성과 다양한 간암세포에서의 증식 억제활성을 조사하였다. 참외 꼭지와 참외 줄기 잎 부위에서 농도의존적으로 QR 유도활성이 증가하였고, $200{\mu}g/mL$ 농도에서는 각각 3.9, 1.5배의 유도활성을 나타내었다. 암세포 사멸 활성 측정법을 통한 항암활성 평가 실험에서 마우스 유래의 간암세포인 Hepa1c1c7 세포에 대해서 조사한 결과 꼭지와 줄기 잎 부위에서 높은 암세포 독성을 보였다. 이러한 결과를 기초로 인체유래의 암세포에 대한 항암활성을 평가하기 위해 인체유래 간암 세포주인 HepG2에 대한 세포 증식 억제활성을 농도별로 조사하였다. 꼭지와 줄기 잎 부위 모두 인체유래 간암 세포에 대해 증식 억제효과를 보여주었지만, 특히 꼭지 부위는 최고농도에서 60.3%의 높은 증식 억제효과를 보였다. 그러나 마우스 유래의 간암세포에 대한 활성보다 인체유래 간암세포에 대한 활성이 낮게 나타났다. 참외의 꼭지 추출물에서 QR 유도활성과 항암활성을 확인함으로써 향후 참외 비가식 부위의 기능성 소재로의 이용화에 대한 연구가 필요할 것으로 생각된다.

Keywords

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