Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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A Molecular Marker Discriminating the Soybean Podworm, Matsumuraeses phaseoli and the Podborer, M. falcana (Lepidoptera: Tortricidae)

팥나방(Matsumuraeses phaseoli)과 어리팥나방(M. falcana)의 판별 분자마커

  • Heo, Hye-Jung (Plant Medicine, School of Bioresource Sciences, Andong National University) ;
  • Son, Ye-Rim (Plant Medicine, School of Bioresource Sciences, Andong National University) ;
  • Seo, Bo-Yoon (National Institute of Crop Science) ;
  • Jung, Jin-Kyo (National Institute of Crop Science) ;
  • Kim, Yong-Gyun (Plant Medicine, School of Bioresource Sciences, Andong National University)
  • 허혜정 (안동대학교 생명자원과학부 식물의학) ;
  • 손예림 (안동대학교 생명자원과학부 식물의학) ;
  • 서보윤 (농촌진흥청 국립식량과학원) ;
  • 정진교 (농촌진흥청 국립식량과학원) ;
  • 김용균 (안동대학교 생명자원과학부 식물의학)
  • Published : 2009.12.30
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Abstract

Two closely related species, the soybean podworm, Matsumuraeses phaseoli, and the podborer, M. falcana, gives differential economic damages on crops. It is difficult to discriminate these potential sympatric species by morphological characters. The goal of this study was to develop a discriminating molecular marker based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A partial genomic fragment (500 bp) of mitochondrial cytochrome oxidase I (mtCOI) was sequenced in both species, in which restriction site by Rsa I was selected as a dichotomous marker. PCR-RFLP in the mtCOI region clearly discriminated both species.

팥나방(Matsumuraeses phaseoli)과 어리팥나방(M. falcana)은 유사한 종으로 작물에 상이한 피해를 주고 있다. 그러나 형태적 특징만으로 이 두 동소적 유사종을 쉽게 구분하기 어렵다. 본 연구는 이 두 종을 뚜렷하게 판별할 수 분자마커를 개발했다. 두 종의 시토크롬 옥시다아제-I의 부분(약 500 bp) 염기서열이 밝혀졌다. 이를 바탕으로 두 종을 구분하는데 이용될 수 있는 판별 제한효소인 Rsa I이 선발되었고 PCR-RFLP를 통해 입증되었다.

Keywords

References

  1. Byun, B.K., K.T. Park and Y.M. Park. 2005. Review of the genus Matsumuraeses Issiki (Lepidoptera, Tortricidae) with discovery of M. falcana (Walsingham) in Korea. J. Asia Pac. Entomol. 8: 117-122 https://doi.org/10.1016/S1226-8615(08)60080-1
  2. Cho, J.R., K.S. Choi, J.K. Jung, J.H. Park and B.Y. Seo. 2007. Development of sex pheromone trap to monitoring Matsumuraeses falcana (Walshingham) (Lepidoptera: Tortricidae). J. Asia Pac. Entomol. 10: 345-349 https://doi.org/10.1016/S1226-8615(08)60374-X
  3. Jung, J.K., B.Y. Seo, J.R. Cho, Y.H. Kwon and G.H. Kim. 2009. Occurrence of lepidopteran insect pests and injury aspects in Adzuki bean fields. Kor. J. Appl. Entomol. 48: 29-35 https://doi.org/10.5656/KSAE.2009.48.1.029
  4. Jung, J.K., B.Y. Seo, J.H. Park, J.K. Moon, B.S. Choi and Y.H. Lee. 2007. Developmental characteristics of soybean podworm, Matsumuraeses phaseoli (Lepidoptera: Tortricidae) and legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae) on semi-synthetic artificial diets. Kor. J. Appl. Entomol. 46: 393-399 https://doi.org/10.5656/KSAE.2007.46.3.393
  5. Kim, Y., M.L. Lee and C. Chung. 1998. Study on the genetic variation of the mitochondrial DNA in the beet armyworm, Spodoptera exigua (Hübner), using PCR-RFLP. Kor. J. Appl. Entomol. 37: 23-30
  6. Kobayashi, T. and T. Oku. 1980. Sampling lepidopterous pod borers on soybean. pp. 422-437. In Sampling methods in soybean entomology, eds. by M. Kogan and D.C. Herzog. 587pp. Springer-Verlag, New York
  7. Loxdale, H.D. and G. Lushai. 1998. Molecular markers in entomology. Bull. Entomol. Res. 88: 577-600 https://doi.org/10.1017/S0007485300054250
  8. Oku, T., Y. Miyahara, T. Fujimura and A. Toki. 1983. Preliminary note Matsumuraeses species (Lepidoptera, Tortricidae) injuring soybeans in Tohoku district. Jpn. J. Appl. Entomol. Zool. 27: 28-34 https://doi.org/10.1303/jjaez.27.28
  9. Sambrook, J.E., F. Fritsh and T. Maniatis. 1989. Molecular cloning. A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
  10. Simon, C., F. Frati, A. Beckenbach, B, Crespi, H. Liu and P. Flook. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am. 87: 651-701 https://doi.org/10.1093/aesa/87.6.651
  11. Wakamura, S. 1985. Identification of sex pheromone components of the podborer, Matsumuraeses falcana (Walsingham) (Lepidoptera: Tortricidae). Appl. Entomol. Zool. 20: 189-198
  12. Wakamura, S. and K. Kegasawa. 1986. Sex pheromone of the podborer, Matsumuraeses falcana (Walsingham) (Lepidoptera: Tortricidae): activity of the third component, (Z,E)-7,9-dodecadienyl acetate, and 3-component formulation. Appl. Entomol. Zool. 21: 334-339

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