흰쥐의 섬유아세포 L-929를 이용한 새로운 Soft Contact Lens 소재의 생물안전성 검증

Biosafety of the New Soft Contact Lens Materials in the Fibroblast L-929 Cell Line

  • 유영현 (경북대학교 자연과학대학 생명공학부) ;
  • 남주형 (경북대학교 자연과학대학 생명공학부) ;
  • 김병길 (경북대학교 자연과학대학 생명공학부) ;
  • 김순복 ((주)미광콘택트렌즈) ;
  • 문익재 ((주)웰진) ;
  • 김종필 ((주)웰진) ;
  • 서영배 (경북대학교 자연과학대학 생명공학부)
  • 발행 : 2009.03.28

초록

In this study, we polymerized new materials for soft contact lens using HEMA (2-hydroxyethyl methacrylate) which is the based-monomer of soft contact lens, EGDMA (ethylene glycol dimethacrylate) as cross linkage agent, and the new additives of monoester or di-ester derived from itaconic acid commercially produced by the fermentation of Asp. itaconicus. New polymer materials for soft contact lens were synthesized with the mixture of HEMA and mono- or diester at different ratios and presented to a good water content and oxygen transmissibility (Dk/L) values. In case of polymerization with HEMA and mono-ester (15%), the water content and oxygen transmissibility of contact lens were found to be good values at 57.6% and 28.5 Dk respectively. The mixture of HEMA and mono-ester is more excellent than HEMA/di-ester in the water content and oxygen transmissibility. The toxicity of new contact lens materials were confirmed in the fibroblast L-929 cell line using a agar overlay test and a growth inhibition test with the extract solution of contact lens.

키워드

참고문헌

  1. Bressler, E. and S. Braun. 2000. Conversion of citric acid to itaconic acid in a novel liquid membrane bioreactor. J, Chem. technol. Biotechnol. 75: 66-72 https://doi.org/10.1002/(SICI)1097-4660(200001)75:1<66::AID-JCTB176>3.0.CO;2-U
  2. Caroline, C.S. Karlgard, D. K. Sarkar, L. W. Jones, and K. T. Leung, 2004. Drying methods for XPS analysis of pure $Vision^{TM}$, Focus $Night&Day^{TM} $and conventional hydrogel contact lenses. A. Surface Science. 230: 106-114 https://doi.org/10.1016/j.apsusc.2004.02.020
  3. Chung, S. Y. and J. I. Rhee. 2002. Production and monitoring of itaconic acid from starchy materials, Korean J. Biotechnol. Bioeing. 17: 477-483
  4. IACLE, 1997. The IACLE Contact lens course, Module 2, 5
  5. Kim, J. H., J. S. Seong, K. C. Yoo, Y. Yoon, M. S. Ra, and J. B. Lee. 1999. Comparison of contact lenses and storage solution for cytotoxic potential using a cel1 growth inhibition assay. J. Korean Oph. Opt. Soc. 4: 81-86
  6. Lai, T. C., Wilson, A. C. and Zantos, S. G. 1993. KirkOthmer Encylopedia of Chemical Technology. 4th ed., John Wiley. New York. 7: 192-218
  7. Park, J. h., S. K. Ju, J. S. Park, Y. K. Park, M. H. Kang and K. H. You. 2004. The effect of overexpression of rat clusterin in L929 fibroblasts. J. Microbiol. biotechnol. 14: 1333-1337
  8. P. White. 1994. Contact lens spectrum. Nov 31-44
  9. P. White and C. Scott. 1999. Contact lens Spectrum Suppl.7
  10. Reddy, C. S. and K., R. P. Singh, 2002. Enhanced production of itaconic acid from com starch and market refuse fruits by genetically manipulated Aspergillus terreus SKR 10, Bioresour. Tech. 85: 69-71 https://doi.org/10.1016/S0960-8524(02)00075-5
  11. Refojo, M. F. 1996. Polymeric Materials encylopedia. CRC Press, New York, 2: 1504-1509
  12. Ruben, M. and Guillon, M., 1994. Contact Lens Practice, Chapman & Hall Medical. London