Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Single Nucleotide Polymorphism Analysis of the COI Gene in Korean Native Chicken

한국재래닭 COI 유전자의 단일염기다형 분석

  • Jin, S.D. (National Science Museum) ;
  • Seo, D.W. (Division of Animal Science and Resources, Chungnam National University) ;
  • Sim, J.M. (Division of Animal Science and Resources, Chungnam National University) ;
  • Baek, W.K. (National Science Museum) ;
  • Jung, K.C. (Division of Animal Science and Resources, Chungnam National University) ;
  • Jang, B.K. (National Institute of Animal Science, R.D.A.) ;
  • Choi, K.D. (The Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, Chungnam National University)
  • Published : 2009.03.31
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Abstract

One of the mitochondrial genes, called cytochrome c oxidase I (COI), has been widely used for the species identification (called bio-barcode) in birds. In this study, the bio-barcode has been applied to chicken breeds in Korea whether it also can be used as a molecular marker for breed identification. Data indicated that Korean native chicken has the mixed SNP (single nucleotide polymorphism) patterns between White Leghorn (Layer) and Cornish (Broiler) and ultimately, it can not be used as the marker for breed identification. However, this result indicates the mixed use of the Korean native chicken, since it has been used for dual purpose for producing meat and egg for a long time. In order to use as a marker for species identification, more reliable mitochondrial and/or nuclear DNA markers need to be developed.

조류에서 미토콘드리아내 유전자인 cytochrome c oxidase I(COI)는 종 구분을 위한 바이오마커로 많이 이용이 되고 있다. 본 연구는 이 유전자의 변이를 이용하여 닭의 품종 구분이 가능한지를 실험하기 위하여 실시하였다. 그 결과 한국 재래닭은 공시축으로 사용한 산란계인 White Leghorn과 육계인 Cornish가 가지고 있는 단일염기다형을 모두 가지고 있는 것으로 판명되어 품종구분을 위한 마커로 사용하기에는 적합하지 않은 것으로 확인되었다. 그러나 본 연구 결과를 통하여 한국재래계가 육계와 산란계의 특성을 모두 가지고 있다는 기존의 학설을 뒷받침하였으며, 품종 구분을 위하여 미토콘드리아와 genomic DNA 유래 단일염기다형 선발의 중요성을 확인할 수 있었다.

Keywords

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