Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effects of Dietary Chitosan Supplementation with Chitosan Feeding Levels and Feeding Forms on Growth Performance and Carcass Characteristics of Thigh Muscular in Broiler

키토산의 급여량과 급여 방법에 따른 육계의 성장 및 닭 다리육의 품질에 미치는 영향

  • Kim, Y.J. (Division of Life Resources, Daegu University) ;
  • Kim, B.K. (Gyeongsangbuk-do Livestock Research Institute)
  • 김영직 (대구대학교 생명자원학부) ;
  • 김병기 (경상북도 축산기술연구소)
  • Published : 2009.03.31
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Abstract

This study was conducted to determine the effects of dietary supplementation with chitosan feeding levels and feeding forms on the performance, proximate composition, pH, thiobarbituric acid reactive substance (TBARS), water holding capacity (WHC), shear force, meat color, and fatty acid of chicken thigh meat. Two hundred broilers (Arbor Acre Broiler, male) were randomly assigned to five groups and were fed for five weeks and slaughtered. Thigh muscle was evaluated in this experiment. The amounts of proximate composition, crude fat of control and T3 for the chitosan-treated groups were significantly higher as compared with T2 (P<0.05), but no significant effects were detected on moisture, crude protein, and crude ash. By comparison, pH was significantly increased when chitosan was included at 2% into diet or more than 1% into drinking water. TBARS was significantly lower at chitosan treated groups it was decreased with increasing chitosan level in the diets (P<0.05). Therefore, chitosan had the possibility to improve shelf life of chicken meat. Higher chitosan levels and feeding additive increased high density lipoprotein cholesterol (HDL-C) and reduced total cholesterol and low density lipoprotein cholesterol (LDL-C) (P<0.05). $L^*$ and $a^*$ of chitosan treated group was higher than the control. As the dietary chitosan levels increase, the composition of palmitic acid and oleic acid levels was increased, however, those but the rates of linoleic acid and arachidonic acid were decreased. It is concluded that dietary chitosan has a positive effect on increasing HDL-C and oleic acid and decreasing total-C, LDL-C and TBARS values. Therefore, the treatment with the most significant effects in the current study was the high level of chitosan.

본 연구는 키토산을 육계 사료에 첨가하지 않은 무첨가구를 대조구로 하고, 사료에 1% 첨가구를 T1, 2% 첨가구를 T2, 음수에 1% 첨가구를 T3, 2% 첨가구를 T4로 첨가 급여하면서 육계의 생산성을 조사하였고, 키토산을 급여한 계육의 일반 성분, 혈청콜레스테롤, pH, TBARS, WHC, 전단력, 육색 및 지방산 조성의 변화를 검토하고자 육계 200수(Arbor Acre Broiler, male)를 공시하여 사양한 후 다리 부위 근육을 이용하여 분석하였다. 육계의 증체량, 사료 요구율 등의 생산성은 차이가 없었다. 계육의 일반 성분 중에 조지방 함량은 대조구와 T3에서 유의적으로 높은 함량을 보였고 (P<0.05), 수분, 조단백질 및 조회분은 키토산 급여에 의한 영향은 없었다. pH는 대조구보다 키토산 급여구에서 높게 나타났다(P<0.05). TBARS는 키토산 급여구에서 유의적으로 낮은 결과를 보였고, 음수에 첨가하는 방법보다는 사료에 첨가하는 방법이 효과적이었다. 키토산 급여량이 증가할수록 낮은 TBARS값을 나타내어(P<0.05), 키토산은 계육의 저장성의 향상 가능성이 보였다. 혈청콜레스테롤은 키토산을 급여함으로써 총콜레스테롤, LDL-C은 감소하고, HDL-C은 증가하고, 첨가량이 많을수록 그리고 음수보다는 사료에 첨가하는 방법이 효과적이었다. 육색은 키토산 급여구에서 $L^*$값과 $b^*$값은 높았고, 지방산은 palmitic acid, oleic acid 등은 증가하고, linoleic acid 및 arachidonic acid는 감소하였다(P<0.05). 결론적으로 키토산을 사료에 첨가 급여함으로 총 콜레스테롤, LDL-C은 감소하고, HDL-C은 증가하며, TBARS값을 낮추는 효과가 있다. 이와 같은 결과는 음수보다는 사료에 첨가하는 방법이 유리하며 첨가량이 많을수록 좋았다.

Keywords

References

  1. Adams JR, Huffman D 1972 Effect of controlled gas automosphers and temperature on quality of packed pork. J Food Sci 37:1869-1875
  2. AOAC 1998 Official Methods of Analysis of the Association of Official Analytical Chemists. 16th ed. Association of Official Analytical Chemists. Washington DC. USA
  3. Asaoka K 1996 Chitin-chitosan. The choice food supplement for over 10,000 physicians in Japan, Vantage Press, Inc. New York, NY USA
  4. Bidlack WR, Tappel AL 1973 Damage to microsomal membrane by lipid peroxidation. Lipids 8:177-178 https://doi.org/10.1007/BF02544631
  5. Chiang MT, Yao HT, Chen HC 2000 Effect of dietary chitosans with different viscosity on plasma lipids and lipid peroxidation in rats fed on a diet enriched with cholesterol. Biosci Biotechnol Biochemi 64:965-971 https://doi.org/10.1271/bbb.64.965
  6. Duncan DB 1955 Mutiple range test. Biometric 11:1-6 https://doi.org/10.2307/3001478
  7. Folch J, Lees M, Sloane-Stanley GH 1957 A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497-507
  8. Friedewald WT, Levy RI, Fredrickson DS 1972 Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 18:1163-1170
  9. Furda I 1990 Interaction of dietary fiber with lipids chanistic theories and limitation. In New Developments in Dietary Fiber. Plenum Press, New York. pp. 67-82
  10. Gordon DT, Williford CB 1983 Chitin and chitosan; influence on element absorption in rats, ACS Symposium Series 214, Unconventional Source of Dietary Fibers 156-184 https://doi.org/10.1021/bk-1983-0214.ch012
  11. Gross WB, Siegel HS 1983 Evaluation of the heperophil/lymphocyte ratio as measure of stress in chickens. Avian Dis 27:972-979 https://doi.org/10.2307/1590198
  12. Grundy SM 1986 Comparison of monounsaturated fatty acid and carbohydrate for lowering plasma cholesterol. Engl J Med 316:745-751
  13. Guha S, Pal SK, Chatterjec N, Sarker G, Pal S, Basu AK, Bancrjee R 2005 Effect of chitosan on lipid levels when administered concurrently with atorvastatin-a placebo controlled study. J Indian Med Assoc 103:418-421
  14. Hood RL 1984 Cellular and biochemical aspects of fat deposition in the broiler chicken. Poult Sci 40:160-164
  15. Hossain S, Rahman A, Kahir Y, Shams AA, Atros F, Ilashimoto M 2007 Effects of shrimp-drived chitosan on plasma lipid profile and liver lipid peroxide levels in hypercholesterolaemic rats. Clin Exp Phamacol Physiol 34:170-176 https://doi.org/10.1111/j.1440-1681.2007.04568.x
  16. Ikeyama H, Morton RJ 1995 Chitosan-healing powder from the sea. Los Angeles, CA, Will Productions
  17. Jean YJ, Kim SK 2001a Effect of antimicrobial activity by chitosan oligosaccharides N-conjugated with asparagine. J Microbiol Biotechnol 11:281-286
  18. Jean YJ, Kim SK 2001b Potential immuno-stimulating effect of antitumoral fraction of chitosan oligosaccarides. J Chitin Chitosan 6:163-167
  19. Johnson IT, Gee JM 1981 Effect of gel forming gums in the intestinal unstirred layer and sugar transport in vitro. Gut 22:398-403 https://doi.org/10.1136/gut.22.5.398
  20. LeHoux JG, Grondin F 1993 Some effects of chitosan on liver function in the rat. Endocrinology 132:1078-1084 https://doi.org/10.1210/en.132.3.1078
  21. Lunt K, Smith SB 1991 Wagyu beefs holds profit potential for U.S. feed lot. Feedstuff 19:18-22
  22. Luong JHT, Nguyen AL, Male KB 1987 Recent developments in downstream processing based on affinity interaction. Trends in Biotechnol 5:281-286 https://doi.org/10.1016/0167-7799(87)90060-6
  23. Moon MS, Lee MS, Kim CT, Kim YH 2007 Dietary chitosan enhances hepatic CYP7A1 activity and reduces plasma and liver cholesterol concentration in diet-induced hypercholesterolmia in rats. Nutrition Research and Practice 1(3):175-179 https://doi.org/10.4162/nrp.2007.1.3.175
  24. Muzzarelli RA 1997 Human enzymatic activities related to the therapeutic administration of chitin derivatives. Cell Mol Life Sci 53:131-140 https://doi.org/10.1007/PL00000584
  25. Muzzarelli RA, Barontini G, Rocchetti R 1976 Immunobilized enzymes on chitosan columns; a-chymotrysin and phosphatase. Biotechnol Bioeng 18:1445-1454 https://doi.org/10.1002/bit.260181011
  26. Razdan A, Pettersson D 1994 Effect of chitosan on nutrient digestibility and plasma lipid concentration in broiler chicken. Br J Nutr 72:277-288 https://doi.org/10.1079/BJN19940029
  27. Saito M 1988 International between lipid peroxide formation and nutritional status. J Jpn Soc Nutr Food Sci 41:343-349 https://doi.org/10.4327/jsnfs.41.343
  28. SAS Institute Inc. 1996 SAS/STAT User's Guide: Version 6. SAS Institute, Inc., Cary, North Carolina
  29. Seo H, Kinemura Y 1998 Preparation and properties of chitosan porous beads, in Proceeding of chitosan porous beads, in Proceeding from the 4th International Conference on Chitin and Chitosan. Vol. 4. pp. 585-588
  30. Soultos N, Tzikas Z, Abrahim A, Georgantelis D, Ambrosiadis I 2008 Chitosan effects on quality properties of Greek style fresh pork sausage. Meat Sci 80:1150-1156 https://doi.org/10.1016/j.meatsci.2008.05.008
  31. Witte VC, Krause F, Baile ME 1970 A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. J Food Sci 35:352-358 https://doi.org/10.1111/j.1365-2621.1970.tb00928.x
  32. Yong KS, Han SO, Mahato RI, Kim SW 2000 Development of biomaterials for gene therapy. Molecular Therapy 2(4):302-317 https://doi.org/10.1006/mthe.2000.0142
  33. 김길남 조은숙 김규일 김세권 양현필 전유진 2005 고콜레스테롤식이에 있어 키토산 올리고당이 체내 콜레스테롤 농도 및 황산화 효소 활성에 미치는 영향. 한국식품영양과학회지 34(1):36-41 https://doi.org/10.3746/jkfn.2005.34.1.036
  34. 김종원 김종덕 성기성 강석남 2003 키토산 발효 사료의 첨가가 비육돈의 도체특성 및 육질에 미치는 영향. 동물자원지 45(3):463-472 https://doi.org/10.5187/JAST.2003.45.3.463
  35. 남기달 한효규 조승주 박길현 김종대 2008 닭 성장 및 육질 개선을 위한 수용성 키토산의 사료 첨가 급이 효과. J Chitin Chitosan 13(1):14-22
  36. 성형철 성용길 2002 키토산의 농축산 분야에의 응용. 한국 키틴키토산학회지 7:179-184
  37. 이유방 성삼경 1984 식육과 육제품의 분석실험. 선진문화사. 서울 pp.129
  38. 정병윤 백인기 이동명 서상봉 2004 키토산 제제 첨가가 육용 종계, 육계, 돼지 및 젖소의 생산성에 미치는 영향. J Chitin Chitosan 9(4):168-174