Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Comparison of Recovery Levels of Staphylococcus aureus Treated at Different NaCl Concentrations after Sublethal Heating

Staphylococcus aureus 의 sublethal heating 후 NaCl 농도에 따른 회복 정도 비교

  • Park, Kyung-Shik (Dept. of Food Science and Technology, Chung-Ang University) ;
  • Kim, Min-Ju (Dept. of Food Science and Technology, Chung-Ang University) ;
  • Jung, Hye-Jin (Dept. of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Dept. of Food Science and Technology, Chung-Ang University)
  • Published : 2009.12.31
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Abstract

The viability of Staphylococcus aureus, a significant cause of food poisoning in Korea, on TSA plates was determined after sublethal heating treatments and NaCl treatments. In addition, recovery levels of sublethally injured cells on TSA plates containing different concentrations of NaCl (TSAS) were investigated. The viability decreased significantly with increasing degree of sublethal heating treatments, but increases in NaCl treatment concentrations from 0 to 6% had little effect on the viability. After being sublethally treated at $55^{\circ}C$ for 30 min, bacterial populations were reduced by 7.91, 7.97 and 7.99 log CFU/mL on 2, 4 and 6% TSAS, respectively. After being sublethally treated at 60oC for 30 min, bacterial populations were reduced by 6.46, 6.47 and 6.48 log CFU/mL on 2, 4 and 6% TSAS, respectively. Decimal reduction times (D-value) decreased with increasing NaCl treatment concentrations after sublethal heating at 55 and $60^{\circ}C$. These data imply that the S. aureus cells sublethally injured by insufficient heating processes had a lower recovery rate with increasing NaCl concentrations in the recovery media.

본 연구에서는 우리나라에서 2-3번째로 자주 발생하는 식중독 원인균인 S. aureus를 대상으로 불충분한 열처리와 NaCl처리에 따른 각각의 생존균수 변화와 불충분한 열처리 후 다양한 NaCl농도(0%, 2%, 4%, 6%)하에서 sublethal injured cell의 회복 정도를 조사하였다. 그 결과, 불충분한 열처리 정도가 증가함에 따라 생존균수가 유의적인 감소를 하였으나, NaCl 처리시 NaCl 농도가 6%까지 증가함에 따라서 생존균수의 변화가 거의 없었다. 그리고 $55^{\circ}C$나 $60^{\circ}C$에서 30분간 열처리하는 기간 동안에 sublethal injured cell들이 발생하였으며, 그들의 회복 정도는 높은 염농도에 노출될수록 회복 정도가 감소하였다. 또한 이와 같은 현상은 같은 온도에서 열처리 시간이 증가할수록 더욱 명확히 나타났다. 그리고 동일한 온도에서 sublethal heating 후 노출된 NaCl 농도가 증가함에 따라서 D-value가 감소하는 경향을 나타내었다. 이와 같은 결과는 불충분한 열처리 과정에 의하여 생성된 sublethal injured cell들이 열처리 후 회복되는 과정에서 배지의 NaCl 농도가 증가함에 따라서 회복 정도가 감소하였음을 의미한다.

Keywords

References

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