Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Synergistic Effects of the Combination of 20-Hydroxyecdysone with Ampicillin and Gentamicin Against Methicillin-Resistant Staphylococcus aureus

  • Kim, Eun-Sook (Vestibulocochlear Research Center and Department of Microbiology, School of Medicine, Wonkwang University School of Medicine) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Kim, Jung-Hoon (Korea Institute of Oriental Medicine) ;
  • Park, Channy (Vestibulocochlear Research Center and Department of Microbiology, School of Medicine, Wonkwang University School of Medicine) ;
  • Kim, Shin-Moo (Department of Clinical Laboratory Science, Wonkwang Health Science University) ;
  • Kim, Jin-Kyung (Vestibulocochlear Research Center and Department of Microbiology, School of Medicine, Wonkwang University School of Medicine) ;
  • Lee, Kang-Min (Division of Biological Sciences, Chonbuk National University) ;
  • Lee, Sang-Heon (Department of Otorhinolaryngology, Wonkwang Medical Science) ;
  • So, Hong-Seob (Vestibulocochlear Research Center and Department of Microbiology, School of Medicine, Wonkwang University School of Medicine) ;
  • Park, Ra-Kil (Vestibulocochlear Research Center and Department of Microbiology, School of Medicine, Wonkwang University School of Medicine)
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has led to an urgent need for the discovery and development of new antibacterial agents. As part of an ongoing investigation into the antibacterial properties of natural products, 20-hydroxyecdysone (20E), isolated from the roots of Achyranthes japonica Nakai, was found to be active against MRSA, either alone or in combination with ampicillin (AM) or gentamicin (GM), via checkerboard assay. This study investigated the antibacterial activity of 20E, which exhibited poor antibacterial activity ($MIC=250-500\;{\mu}g/ml$) against MRSA tested. The combined activity of AM or GE plus 20E against MRSA resulted in fractional inhibitory concentractions (FICs) ranging from 4.00 to $0.031\;{\mu}g/ml$, respectively. Meanwhile, the FIC index ranged from 0.16-4.50, indicating a marked synergistic relationship between AM, GE, and 20E against MRSA. Time-kill assays also showed a remarkable decrease between the combination and the more active compound. Therefore, this study demonstrated that AM, GE, and 20E can act synergistically in inhibiting MRSA in vitro.

Keywords

References

  1. Baker, C. N., C. Thornsberry, and R. R. Facklam. 1981. Synergism, killing kinetics, and antimicrobial susceptibility of group A and B streptococci. Antimicrob. Agents Chemother. 19: 716-725 https://doi.org/10.1128/AAC.19.5.716
  2. Catalan, R. E., A. M. Martinez, M. D. Aragones, B. G. Miguel, A. Robles, and J. E. Godoy. 1985. Alterations in rat lipid metabolism following ecdysterone treatment. Comp. Biochem. Physiol. B 81: 771-775 https://doi.org/10.1016/0305-0491(85)90403-1
  3. Choi, H. S., M. J. Lee, M. S. Na, M. Y. Lee, and D. B. Choi. 2009. Antioxidant properties of Achyranthis radix extract in rats. J. Ind. Eng. Chem. 15: 275-280 https://doi.org/10.1016/j.jiec.2008.12.001
  4. Clinical and Laboratory Standards Institute. 2006. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard, 7th Ed. Document M7-A7. Clinical and Laboratory Standards Institute, Villanova, PA, U.S.A
  5. Clinical and Laboratory Standards Institute. 2009. Methods For Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard, 9th Ed. Document M100-S19. Clinical and Laboratory Standards Institute, Villanova, PA, U.S.A.
  6. Coticchia, J. M. and J. E. Dohar. 2005. Methicillin-resistant Staphylococcus aureus otorrhea after tympanostomy tube placement. Arch. Otolaryngol. Head Neck Surg. 131: 868-873 https://doi.org/10.1001/archotol.131.10.868
  7. Cusimano, F., V. C. Cocita, and A. D. Amico. 1989. Sensorineural hearing loss in chronic otitis media. J. Laryngol. Otol. 103: 158-163 https://doi.org/10.1017/S0022215100108333
  8. Engel, F., R. Blatz, J. Kellner, M. Palmer, U. Weller, and S. Bhadki. 1995. Breakdown of the round window membrane permeability barrier evoked by streptolysin O: Possible etiologic role in development of sensorineural hearing loss in acute otitis media. Infect. Immun. 63: 1305-1310
  9. Engelmann, F. 1971. 20-Hydroxyecdysone, what it can do. Science 174: 1041
  10. Guo, J., Y. Wu, W. Chen, and J. Lin. 1994. Endotoxic damage of the stria vascularis: The pathogenesis of sensorineural hearing loss secondary to otitis media. J. Laryngol. Otol. 108: 310-313 https://doi.org/10.1017/S0022215100126623
  11. Harada, T., T. Yamasoba, and M. Yagi. 1992. Sensorineural hearing loss associated with otitis media with effusion. ORL J. Otorhinolaryngol. Relat. Spec. 54: 61-65 https://doi.org/10.1159/000276263
  12. Heslop, A. and T. Ovesen. 2006. Severe acute middle ear infections: Microbiology and treatment. Int. J. Pediatr. Otorhinolaryngol. 70: 1811-1816 https://doi.org/10.1016/j.ijporl.2006.06.009
  13. Huang, M., D. Dulon, and J. Schacht. 1990. Outer hair cells as potential targets of inflammatory mediators. Ann. Otol. Rhinol. Laryngol. Suppl. 148: 35-38
  14. Housley, G. P., C. H. Norris, and P. S. Guth. 1988. Histamine and related substances influence neurotransmission in the semicircular canal. Hear. Res. 35: 87-98 https://doi.org/10.1016/0378-5955(88)90043-3
  15. J$\acute{o}$kay, I., Z. Papp, G. So$\acute{o}$s, I. Sziklai, and B. Dezs$\ddot{o}$. 2001.The effect of chronic otitis media on the immunoreactivity of human inner ear. Eur. Arch. Otorhinolaryngol. 258: 529-532 https://doi.org/10.1007/s004050100384
  16. Lee, Y. S., O. H. Kang, J. G. Choi, Y. C. Oh, H. S. Chae, J. H. Kim, et al. 2008. Synergistic effects of the combination of galangin with gentamicin against methicillin-resistant Staphylococcus aureus. J. Microbiol. 46: 283-288 https://doi.org/10.1007/s12275-008-0012-7
  17. Lafont, R., J. Harmatha, F. Marion-Poll, L. Dinan, and I. D. Wilson. 2002. Ecdybase - The Ecdysone Handbook. 3rd Ed. Continuosly updated online at http://ecdybase.org
  18. Mates, S. M., E. S. Eisenberg, L. J. Mandel, L. Patel, H. R. Kaback, and M. H. Miller. 1982. Membrane potential and gentamicin uptake in Staphylococcus aureus. Proc. Natl. Acad. Sci. U.S.A. 79: 6693-6697 https://doi.org/10.1073/pnas.79.21.6693
  19. Miranda-Novales, G., B. E. Leanos-Miranda, M. Vilchis-Perez, and F. Solórzano-Santos. 2006. In vitro activity effects of combinations of cephalothin, dicloxacillin, imipenem, vancomycin and amikacin against methicillin-resistant Staphylococcus spp. strains. Ann. Clin. Microbiol. Antimicrob. 5: 25 https://doi.org/10.1186/1476-0711-5-25
  20. Nascimento, A. M., M. G. Brandao, G. B. Oliveira, I. C. Fortes, and E. Chartone-Souza. 2007. Synergistic bactericidal activity of Eremanthus erythropappus oil or beta-bisabolene with ampicillin against Staphylococcus aureus. Antonie Van Leeuwenhoek 92: 95-100 https://doi.org/10.1007/s10482-006-9139-x
  21. Pajor, A., M. Durko, A. Jankowski, A. Bartoszko-Tyczkowska, and A. Sta$\acute{n}$czyk. 2006. Bacteriological evaluation in chronic otitis media. Otolaryngol. Pol. 60: 757-763
  22. Paparella, M. M., T. Morizono, C. T. Le, F. Mancini, P. Sipil$\ddot{a}$, Y. B. Choo, G. Liden, and C. S. Kim. 1984. Sensorineural hearing loss in otitis media. Ann. Otol. Rhinol. Laryngol. 93: 623-629
  23. Ryffel, C. W., I. Tesch, P. E. Birch-Machin, L. Reynolds, F. H. Barberis-Maino, H. Kayser, and B. Berger-B$\ddot{a}$chi. 1990. Sequence comparison of mecA genes isolated from methicillinresistant Staphylococcus aureus and Staphylococcus epidermidis. Gene 28: 137-138
  24. Schlievert, P. M., K. N. Shands, B. B. Dan, G. P. Schmid, and R. D. Nishimura. 1981. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxicshock syndrome. J. Infect. Dis. 143: 509-516
  25. Shahverdi, A. R., A. Fakhimi, G. Zarrini, G. Dehghan, and M. Iranshahi. 2007. Galbanic acid from Ferula szowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus. Biol. Pharm. Bull. 30: 1805-1807 https://doi.org/10.1248/bpb.30.1805
  26. Tsuji, B. T. and M. J. Rybak 2005. Short-course gentamicin in combination with daptomycin or vancomycin against Staphylococcus aureus in an in vitro pharmacodynamic model with simulated endocardial vegetations. Antimicrob. Agents Chemother. 49: 2735-2745 https://doi.org/10.1128/AAC.49.7.2735-2745.2005
  27. Zheng, Y., B. Liu, M. Chen, and T. Chen. 2008. Supercritical fluid extraction of ecdysterone from the roots of Achyranthes bidentata BL. J. Sep. Sci. 31: 1393-1398 https://doi.org/10.1002/jssc.200700468

Cited by

  1. Structural characterization and surface activities of biogenic rhamnolipid surfactants from Pseudomonas aeruginosa isolate MN1 and synergistic effects against methicillin-resistant Staphylococcus aure vol.57, pp.6, 2009, https://doi.org/10.1007/s12223-012-0164-z
  2. 오배자, 우슬, 가자 및 감초를 포함하는 혼합추출물의 항산화 및 항균활성 vol.29, pp.1, 2009, https://doi.org/10.7841/ksbbj.2014.29.1.29
  3. In vitro synergism of magnolol and honokiol in combination with antibacterial agents against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) vol.15, pp.None, 2009, https://doi.org/10.1186/s12906-015-0938-3
  4. Plant-derived antimicrobials to fight against multi-drug-resistant human pathogens vol.7, pp.3, 2009, https://doi.org/10.1007/s13205-017-0848-9
  5. Synthesis, physicochemical, and biological activities of novel N‐acyl tyrosine monomeric and Gemini surfactants in single and SDS/CTAB–mixed micellar system vol.30, pp.10, 2009, https://doi.org/10.1002/poc.3675
  6. 항 Methicillin Resistant Staphylococcus aureus 활성을 가지는 해양미생물 Pseudomonas sp. YJ-1의 분리와 특성 vol.35, pp.4, 2009, https://doi.org/10.11626/kjeb.2017.35.4.694
  7. Synergistic effects of anti-MRSA herbal extracts combined with antibiotics vol.15, pp.13, 2009, https://doi.org/10.2217/fmb-2020-0001