Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Isolation and Molecular Phylogeny of Three Muscle Actin Isoforms of an Endangered Freshwater Fish Species Hemibarbus mylodon (Cypriniformes; Cyprinidae)

  • Published : 2009.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

The Korean doty barbel Hemibarbus mylodon (Cypriniformes; Cyprinidae) is a critically endangered freshwater fish species mainly because of its natural habitat degradation. Three full-length complementary DNA (cDNA) clones representing different muscle actin isoforms were isolated and characterized. The three muscle actin isoforms were 1,294-1,601 bp long with the identical open reading frames of 1,134 bp with the deduced amino acid residues of 377. They showed 83.9-87.2% identities in the coding nucleotide level and 96.8-98.1% identities in the amino acid level. Phylogenetic analysis with the coding nucleotide sequences revealed that three muscle actin isoforms of H. mylodon formed strongly supported monophyletic groups with one of cypriniform skeletal $\alpha$-actin (acta1), cypriniform aortic $\alpha$-actins (acta2), and uncharacterized Danio rerio muscle actin isoform/Salmo trutta slow muscle actin (a novel muscle actin type). Our phylogenetic tree further suggested that cypriniform acta2 only showed the orthologous relationship to tetrapod acta2. Other multiple actin isoforms from diverse teleostean taxa were however clustered to no tetrapod orthologs, i.e., acta1, cardiac $\alpha$-actins (aetc1), acta2, and enteric $\gamma$-actin (actg2). This result strongly suggested that teleostean muscle actins have experienced different and complicated evolutionary history in comparison to mammalian counterparts.

Keywords

References

  1. Bang, I.-C., Y. H. Lim, Y. S. Cho, S. Y. Lee and Y. K. Nam, 2007. Survey of expressed sequence tags from tissue-specific cDNA libraries in Hemibarbus mylodon, an endangered fish species. J. Aquacult., 20, 248-254 (in Korean)
  2. Felsenstein, J., 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783-791 https://doi.org/10.2307/2408678
  3. Gunning, P., P. Ponte, H. Okayama, J. Engel, H. Blau and L. Kedesi, 1983. Isolation and characterization of full-length cDNA clones for human $\alpha$-, $\beta$-, and $\gamma$-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Mol. Cell. Biol., 3, 787-795
  4. Hall, T. A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser., 41, 95-98
  5. Jang, M.-H., M. C. Lucas and G-J. Joo, 2003. The fish fauna of mountain streams in South Korean national parks and its significance to conservation of regional freshwater fish biodiversity. Biol. Conserv., 114, 115-126 https://doi.org/10.1016/S0006-3207(03)00016-8
  6. Kim, I.-S., Y. Choi, C.-L. Lee, Y.-J. Lee, B.-J. Kim and J.-H. Kim, 2005. Illustrated Book ofKorean Fishes. Kyo-Hak Publishing Co. Ltd., Seoul, 615 pp. (in Korean)
  7. Kim, W.-J., Y.-A Lee and I.-C. Bang, 2007. Isolation and characterization of polymorphic microsatellite markers for the endangered Korean freshwater fish Hemibarbus mylodon. Mol. Ecol. Notes, 7, 516--518 https://doi.org/10.1111/j.1471-8286.2006.01639.x
  8. Kim, K.-Y., S. Y. Lee, Y. S. Cho, I.-C. Bang, D. S. Kim and Y. K. Nam, 2008. Characterization and phylogeny of two $\beta$-cytoskeletal actins from Hemibarbus mylodon Cyprinidae, Cypriniformes), a tlrreatened fish species in Korea. DNA Seq., 19, 87-97 https://doi.org/10.1080/10425170701445691
  9. Kim, S., A. Karsi, R. A. Dunham and Z. Liu, 2000. The skeletal muscle $\alpha$-actin gene of channel catfish (Ictalurus punctatus) and its association with piscine specific SINE elements. Gene, 252, 173-181 https://doi.org/10.1016/S0378-1119(00)00198-0
  10. Krasnov, A., H. Teerijoki, Y. Gorodilov and H. Molsa, 2003. Cloning of rainbow trout (Oncorhynchus mykiss) a-actin, myosin regulatory light chain genes and the 5'-flanking region of $\alpha$-tropomyosin: functional assessment of promoters. J. Exp. Biol., 206, 601-608 https://doi.org/10.1242/jeb.00116
  11. Lee, Y.-A, Y-E. Yun, Y. K. Nam and I.-C. Bang, 2008. Genetic diversity of endangered fish Hemibarbus mylodon (Cyprinidae) assessed by AFLP. J. Aquacult., 21, 196-200
  12. Miwa, T., Y. Manabe, K. Kurokawa, S. Kamada, N. Kanda, G Bruns, H. Ueyama and T. Kakunaga, 1991. Structure, chromosome location, and expression of the human smooili muscle (enteri type) $\gamma$-actin gene: evolution of six human actin genes. Mol. Cell. Biol., 11, 3296-3306
  13. Morita, T., 2000. Amino acid sequences of $\alpha$-skeletal muscle actin isoforms in two species of rattail fish, Co.ηphaenoides acrolepis and Coryphaenoides cinereus. Fish. Sci., 66, 1150-1157 https://doi.org/10.1046/j.1444-2906.2000.00182.x
  14. Morita, T., 2003. Structure-based analysis of high pressure adaptation of $\alpha$-actin. J. Biol. Chem., 278, 28060-28066 https://doi.org/10.1074/jbc.M302328200
  15. Moutou, K. A., S. Socorro, D. M. Power, Z. Mamuris and A. V. M. Canario, 2001. Molecular cloning and sequence of gilthead sea bream (Sparus aurata) $\alpha$-skeletal actin: tissue and devel-opmental expression. Biochem. Physiol. B, 130, 13-21 https://doi.org/10.1016/S1096-4959(01)00381-5
  16. Mudalige, W. A. K. A., D. M. Jackman, D. M. Waddleton and D. H. Heeley, 2007. A vertebrate slow skeletal muscle actin iso-form. FEBS J., 274, 3452-3461 https://doi.org/10.1111/j.1742-4658.2007.05877.x
  17. Nylander, J. A. A., 2004. MrModeltest v2. Program distribtued by the author. Evolutionary Centre, Uppsala University
  18. Pollard, T.D. and J. A. Cooper, 1986. Actin and actin-binding proteins:a critical evaluation of mechanisms and functions. Annu. Rev. Biochem., 55, 987-1035 https://doi.org/10.1146/annurev.bi.55.070186.005011
  19. Ronquist, F. and J. P. Huelsenbeck, 2003. MRBA YES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572-1574 https://doi.org/10.1093/bioinformatics/btg180
  20. Sheff, D. R. and P. A. Rubenstein, 1992. Isolation and characterisation of the rat liver actin N-acetyaminopeptidase. J. Biol Chem., 267, 20217-20224
  21. Swofford, D. L., 2002. PAUP: Phylogenetic Analysis Using Parsimony (and Other Methods). Ver 4.0b10, Sinauer Associates, Sunderland
  22. Tanka, H., Y. Maezawa, T. Ojima and K. Nishita, 2004. Cloning and sequencing of cDNAs encoding walleyc pollack $\alpha$-skeletal actin isoforms. Fish. Sci., 70, 198-200 https://doi.org/10.1111/j.1444-2906.2003.00791.x
  23. Vandekerckhove, J. and K. Weber, 1979. The completε amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal musele: a protein-chemical analysis of muscle actin ifferentiation. Differentiation, 14, 123-133 https://doi.org/10.1111/j.1432-0436.1979.tb01021.x
  24. Venkatesh, B, B. H. Tay, G. EIgar and S. Brenner, 1996. Isolation, characterization and cvolution of nine pufferfish (Fugurubripes) actin genes. J. Mol. Biol., 259, 655-665 https://doi.org/10.1006/jmbi.1996.0347
  25. Watabe, S., Y. Hirayama, J. Imai, K. Kikuchi and M. Yamashita, 1995. Sequences of cDNA clones encoding a-actin of carp and goldfish skcletal muscles. Fish. Sci., 61, 998-1003
  26. Xu, Y., J. He, X. Wang, T. M. Lim and Z. Gong, 2000. Asynchronous activation of 10 muscle-specific protein (MSP) genes during zebrafish somitogenesis. Dev. Dyn., 219, 201-215 https://doi.org/10.1002/1097-0177(2000)9999:9999<::AID-DVDY1043>3.3.CO;2-9