Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Physiological Responses of Dark-banded Rockfish Sebastes inermis to Anesthetization with Clove Oil

  • Park, Min-Ouk (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Ji, Lifeng (Key Laboratory of Mariculture and Biotechnology Agriculture Ministry, Dalian Fisheries University) ;
  • Gil, Hyun-Woo (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Kim, Dong-Soo (Department of Aquaculture, Pukyong National University) ;
  • Park, In-Seok (Division of Marine Environment and Bioscience, Korea Maritime University)
  • Published : 2009.02.25
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Abstract

In order to establish optimum anesthesia concentration, we tested the efficacy of clove oil at five different concentrations in large sized (mean SL $17.1{\pm}2.21\;cm$) and small sized (mean SL $0.6{\pm}0.06\;cm$) dark-banded rockfish Sebastes inermis. Optimal anesthesia concentration for dark-banded rockfish was $150\;mgL^{-1}$ in both large and small sized fish. In general, fish exposed to higher anesthetic doses were rapidly induced but took longer to recover (P<0.05). Recovery time of small sized fish was longer than large sized fish in lower concentrations, while recovery time of large sized fish was longer than small sized fish in higher concentration (P<0.05). Using the established optimum aesthetic concentration, we evaluated the physiological response of dark-banded rockfish to clove oil by measuring plasma cortisol and glucose levels. Following administration of $150\;mgL^{-1}$ clove oil at $20^{\circ}C$ (optimum breeding temperature), plasma cortisol level was highest ($42.2{\pm}11.318{\mu}g/dL$) after 0 hour, while plasma glucose level was highest ($52.5{\pm}10.61\;mg/dL$) after 1 hour. Plasma cortisol and glucose concentrations required 6 and 2 hours, respectively, to return to pre-exposure levels.

Keywords

References

  1. Barton, B. A. and G K. IWanla, 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu. Rev. Fish Dis., 1, 3-26 https://doi.org/10.1016/0959-8030(91)90019-G
  2. Chang, Y. J. and J. W. Hur, 1999. Physiological responses of grey mullet (Mugil cephalus) and Nile tilapia (Oreochromis niloticus) by rapid changes in salinity of rearing water. J. Korean Fish. Soc., 32, 310-316. (in Korean with an English abstract)
  3. Cho, G K. and D. D. Heath, 2000. Comparison of tricaine methanesulphonate (MS-222) and clove oil anaesthesia effects on the physiology of juvenile Chinook salmon Oncorhynchus tshawytscha (Walbaum). Aquacult. Res., 31, 537-546 https://doi.org/10.1046/j.1365-2109.2000.00478.x
  4. Choi, Y., J. H. Kim and J .Y. Park, 2002. Marine Fishes of Korea. Kyo-Hak Publishing Co., Ltd., Seoul, 165 pp
  5. Choi, H. J., K. P. Hong, S. Y. Oh, C. H. Noh, Y. J. Park, J. G Myoung, J. M. Kim, J. W. Hur, C. I. Zhang and I. S. Park, 2005. Growth characteristics of cultured dark-banded rockfish, Sebates inernis Cuvier. J. Aquacult., 18, 147-153
  6. Donaldson, E. M., 1981. The pituitary-interrenal axis as an indicator of stress in fish. (in) A. D. Pickering (ed.), Stress in Fish. Academic Press, London, UK, pp. 11-47
  7. Duncan, D. B., 1955. Multiple-range and multiple F tests. Biometrics, 1, 1-42
  8. Gwak, W. O. and D. W. Park, 2006. Developmental changes in digestive enzymes activity of black rockfish Sebastes inermis. J. Aquacult., 19, 125-132
  9. Gilderhus, P. A. and L. L. Marking, 1987. Comparative efficacy of quinaldine sulfate: MS-222 mixtures for the anesthetization of freshwater fish. U.S. Fish and Wildlife Service, Investigations in Fish Control, pp. 59
  10. Kang, E. J., E. M. Kim, Y. J. Kim, S. G Lim, D. S. Sim, Y. H. Kim and I. S. Park, 2005. Effect of lidocaine hydrochloride and clove oil as an anaesthetic on Korean rose bitterling, Rhodeus uyekii and oily bitterling, Acheilognathus koreensis. J. Aquacult., 18, 272-279. (in Korean with an English abstract)
  11. Massee, K. C., M. B. Rust, R.W. Hardy and R. R. Stickney, 1995. The effectiveness of tricaine, quinaldine sulfate and metomidate as anesthetics for larval fish. Aquaculture, 134, 351-359 https://doi.org/10.1016/0044-8486(95)00057-9
  12. Mattson, N. S. and T. H. Riple, 1989. Metomidate, a better anesthetic for cod (Gadus morhua) in comparison with benzocaine, MS-222, chlorobutanol, and phenoxyethanol. Aquaculture, 83, 89-94 https://doi.org/10.1016/0044-8486(89)90063-X
  13. Oh, S. Y. and C. H. Noh, 2006. Effects ofwater temperature and photoperiod on the oxygen consumption rate of juvenile darkbanded rockfish, Sebastes inermis. J. Aquacult., 19, 210-215
  14. Park, I. S., J. H. JO, S. J> Lee, Y. A. Kim, K. E. Park, J. W. Hur, J. S. YOO and Y. C. Song, 2003. Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate and MS-222 on the greenling (Hixagrammos otakii). J. Korean Fish. Soc., 36, 449-453. (in Korena with an English abstract)
  15. Park, M. O., W. J. Hur, S. Y. Im, D. W. Seol, J. H. Lee and I. S. Park, 2008. Anaesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus. Aquacult. Res., 39, 877-884 https://doi.org/10.1111/j.1365-2109.2008.01941.x
  16. Raabo, E. and T. C. Terkildsen, 1960. On the enzymatic detennination of blood glucose. Scand. J. Invest., 12, 402-407 https://doi.org/10.3109/00365516009065404
  17. Schreck, C. B., 1982. Stress and rearing of salmonids. Aquaculture, 28, 241-249 https://doi.org/10.1016/0044-8486(82)90026-6
  18. Seol, D. W., J. Lee S. Y. Im and I. S. Park, 2007. Clove oil as an anaesthetic for common octopus (Octopus minor, Sasaki). Aquacult. Res., 38, 45-49 https://doi.org/10.1111/j.1365-2109.2006.01622.x
  19. Son, M. H., M. W. Park, J. I. Myeong，D. J. Kim, B. H. Kim, Q. T. Jo and I. G Jeon, 2001. Anaesthetic tolerance of juvenile black rockfish Sebastes schlegeli, produced for wild stock enhancement. Ocean Polar Res., 23, 285-290
  20. Soto, C. G and S. Burhanuddin, 1995. Clove oil as a fish anesthetic for measuring length and weight of rabbitfish (Siganus lineatus). Aquaculture, 136, 149-152 https://doi.org/10.1016/0044-8486(95)01051-3
  21. Summerfelt, R. C. and L. S. Smith, 1990. Anesthesia, surgery, and related techniques. (in) C. B. Schreck and P. B. Moyle (eds.), Methods for Fish Biology. American Fisheries Society, Bethesda Marland， pp. 213-272
  22. Tsantilas, H., A. D. Galatos, F. Athanassopoulou, N. N. Prassinos and K. Kousoulaki, 2006. Efficacy of 2-phenoxyethanol as an anaεsthetic for two size classes of white sea bream, Diplodza sargus L., and sharp snout sea bream, Diplodus puntazzo C. Aquaculture, 253, 64-70 https://doi.org/10.1016/j.aquaculture.2005.07.034
  23. Waterstrat, P. R., 1999. Induction and recovery from anesthesia in channel catfish Ictalurus punctatus fingerlings exposed to clove oil. J. World Aquacult. Soc., 30, 250-255 https://doi.org/10.1111/j.1749-7345.1999.tb00872.x
  24. Westerfield, M., 1993. The Zebrafish Book. University of Oregon Press, Eugene, OR., 342 pp
  25. Woody, C. A., J. Nelson and K. Ramstad, 2002. Clove oil as an anesthetic for adult sockeye salmon: field trials. J. Fish Biol., 60, 340-347 https://doi.org/10.1111/j.1095-8649.2002.tb00284.x
  26. Woolsey, J., M. Holcomb and R. L. Ingennann, 2004. Effect of temperaturε on clove oil anesthesia in steelhead fry. N. Am. J. Aquacult., 66, 35-41 https://doi.org/10.1577/A03-008