DOI QR코드

DOI QR Code

Essential Oil Prepared from Cymbopogon citrates Exerted an Antimicrobial Activity Against Plant Pathogenic and Medical Microorganisms

  • Jeong, Mi-Ran (Research Center of Bioactive Materials and Division of Biological Sciences, Chonbuk National University) ;
  • Park, Pyeong-Beom (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Kim, Dae-Hyuk (Research Center of Bioactive Materials and Division of Biological Sciences, Chonbuk National University) ;
  • Jang, Yong-Suk (Research Center of Bioactive Materials and Division of Biological Sciences, Chonbuk National University) ;
  • Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, Kunsan National University)
  • Published : 2009.03.31

Abstract

Essential oils are mixtures of volatile, lipophilic compounds originating from plants. Some essential oils have useful biological activities including antimicrobial, spasmolytic, antiplasmodial, and insect-repelling activities. In this study, we tested the antimicrobial activity of essential oil prepared from the aromatic plant, Cymbopogon citrates, against three important plant pathogenic and medical microorganisms, Pectobacterium carotovorum, Colletotrichum gloeosporioides, and Aspergillus niger. It effectively inhibited the growth of the bacterium, Pectobacterium carotovorum, in a dose-dependent fashion, and 0.5% of the oil inhibited the growth of bacteria completely. Similarly, the essential oil inhibited the growth of plant pathogenic fungus, Colletotrichum gloeosporioides, and the addition of 1% of essential oil completely inhibited the growth of fungus even after 5 days of culture. Finally, it effectively inhibited the growth of the medically and industrially important fungal species, Aspergillus spp. These results suggest that the essential oil from Cymbopogon citrates may be an environmentally safe alternative to inhibit antimicrobial agents for various uses.

Keywords

References

  1. Blanco, M. M., Costa, C. A. R. A. A. O., Freire, A. O., Santos Jr., J. G. and Costa, M. 2009. Neurobehavioral effect of essential oil of Cymbopogon citrates in mice. Phytomedicine 16:265-270 https://doi.org/10.1016/j.phymed.2007.04.007
  2. Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods - a review. Int. J. Food Microbiol. 94:223-253 https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  3. Burt, S. A., Vlielander, R., Haagsman, H. and Veldhuizen, P. E. J. 2005. Increase in activity of essential oil components carvacrol and thymol against Escherichia coli O157:H7 by addition of food stabilizers. J. Food Prot. 68:919-926 https://doi.org/10.4315/0362-028X-68.5.919
  4. Cha, J. D., Jeong, M. R., Jeong, S. I., Moon, S. E., Kim, J. Y., Kil, B. S. and Song, Y. H. 2005a. Chemical composition and antimicrobial activity of the essential oil of Artemisia scoparia and A. capillaris. Planta Med. 71:186-190 https://doi.org/10.1055/s-2005-837790
  5. Cha, J. D., Jeong, M. R., Choi, H. J. S., Jeong, S. I., Moon, S. E., Yun, S. I., Kim, Y. H., Kil, B. S. and Song, Y. H. 2005b. Chemical composition and antimicrobial activity of the essential oil of Artemisia lavandulaefolia. Planta Med. 71:575-577 https://doi.org/10.1055/s-2005-864164
  6. Crowell, P. L. 1999. Prevention and therapy of cancer by dietary monoterpenes. J. Nutr. 129:775S-778S https://doi.org/10.1093/jn/129.3.775S
  7. Delgado Ayza, C. 2005. What is aromatherapy? Rev. Enferm. 28:55-58
  8. Didry, N., Dubreuil, L. and Pinkas, M. 1993. Antibacterial activity of thymol, carvacrol and cinnamaldehyde alone or in combination. Pharmazie 48:301-304
  9. Di Stasi, L. C., Santos, E. M. G. and Hiruma, C. A. 1989. Plantas medicinais na Amazonia. UNESP, Sao Paulo, p. 194
  10. Duke, J. A. 1989. Handbook of medical herbs, 7th ed., CPR Press, FL, U.S.A.
  11. Edris, A. E. 2007. Pharmaceutical and therapeutic potentials oessential oils and their individual volatile constituents: A review. Phytother. Res. 21:308-323 https://doi.org/10.1002/ptr.2072
  12. Irkin, R. and Korukluoglu, M. 2009. Effectiveness of Cymbopogon citratus L. essential oil to inhibit the growth of some filamentous fungi and yeasts. J. Med. Food 12:193-197 https://doi.org/10.1089/jmf.2008.0108
  13. Juneja, V. K. and Friedman, M. 2007. Carvacol, cinnamaldehyde, oregano oil, and thymol inhibit Clostridium perfringens spore germination and outgrowth in ground turkey during chilling. J. Food. Prot. 70:218-222 https://doi.org/10.4315/0362-028X-70.1.218
  14. Kalemba, D. and Kunicka, A. 2003. Antibacterial and antifungal properties of essential oils. Curr. Med. Chem. 10:813-829 https://doi.org/10.2174/0929867033457719
  15. Kim, M.-K., Choi, G.-J. and Lee, H.-S. 2003. Fungicidal property of Curcuma longa L. rhizome-derived curcumin against phytopathogenic fungi in a greenhouse. J. Agric. Food Chem. 51:1578-1581 https://doi.org/10.1021/jf0210369
  16. Kumaran, A. M., D'Souza, P. Agarwal, A., Bokkolla, R. M. and Balasubramaniam, M. 2003. Geraniol, the putative anthelmintic principles of Cymbopogon martini. Phytother. Res. 17:957 https://doi.org/10.1002/ptr.1267
  17. Kurita, N., Makoto, M., Kurane, R. and Takahara, Y. 1981. Antifungal activity of components of essential oils. Agric. Biol. Chem. 45:945-952
  18. Nevas, M., Korhonen, A. R., Lindstrom, M., Turkki, P. and Korkeala, H. 2004. Antibacterial efficiency of Finnish spice essential oils against pathogenic and spoilage bacteria. J. Food Prot. 67:199-202 https://doi.org/10.4315/0362-028X-67.1.199
  19. Nogueira, J. C., Diniz Mde, F. and Lima, E. O. 2008. In vitro antimicrobial activity of plants in acute otitis externa. Braz. J. Otorhinolaryngol. 74:118-124 https://doi.org/10.1016/S1808-8694(15)30761-8
  20. Olaniyi, A. A., Sofowora, E. A. and Oguntimehin, B. O. 1975. Phytochemical investigation of some Nigerian plants used against fevers. II. Cymbopogon citratus. Planta Med. 28:186-189
  21. Oussalah, M., Caillet, S. and Lacroix, M. 2006. Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of Escherichia coli O157:H7 and Listeria monocytogenes. J. Food Prot. 69:1046-1055 https://doi.org/10.4315/0362-028X-69.5.1046
  22. Paranagama, P. A., Abeysekera, K. H., Abeywickrama, K. and Nugaliyadde, L. 2003. Fungicidal and anti-aflatoxigenic effects of the essential oil of Cymbopogon citratus (DC.) Stapf. (lemon grass) against Aspergillus flavus Link. isolated from stored rice. Lett. Appl. Microbiol. 37:86-90 https://doi.org/10.1046/j.1472-765X.2003.01351.x
  23. Puatanachokchai, R., Kishida, H., Denda, A., Murata, N., Konishi, Y., Vinitketkumnuen, U. and Nakae, D. 2002. Inhibitory effects of lemon grass (Cymbopogon citratus, Stapf) extract on the early phase of hepatocarcinogenesis after initiation with diethylnitrosamine in male Fischer 344 rats. Cancer Lett. 183:9-15 https://doi.org/10.1016/S0304-3835(02)00111-8
  24. Rommelt, H., Zuber, A., Dimagi, K. and Drexel, H. 1974. The absorption of terpenes from bath additives. Munch. Med. Wochenschr. 116:537-540
  25. Sheweita, S. A., Newairy, A. A., Mansour, H. A. and Yousef, M. I. 2002. Effect of some hypoglycemic herbs on the activity of phase I and II drug-metabolizing enzymes in alloxaninduced diabetic rats. Toxicology 174:131-139 https://doi.org/10.1016/S0300-483X(02)00048-3
  26. Tortoriello, J. and Romero, O. 1992. Plants used by Mexican traditional medicine with presumable sedative properties: an ethnobotanical approach. Arch. Med. Res. 23:111-116
  27. Trombetta, D., Castelli, F., Sarpietro, M. G., Venuti, V., Cristani, M., Daniele, C., Saija, A., Mazzanti, G. and Bisignano, G. 2005. Mechanisms of antibacterial action of three monoterpenes. Antimicrob. Agents Chemother. 49:2474-2478 https://doi.org/10.1128/AAC.49.6.2474-2478.2005

Cited by

  1. Óleos essenciais e extratos vegetais no controle da podridão mole em alface crespa vol.30, pp.4, 2012, https://doi.org/10.1590/S0102-05362012000400012
  2. Essential oil composition, insecticidal and antibacterial activities of Salvia tomentosa Miller vol.22, pp.2, 2013, https://doi.org/10.1007/s00044-012-0075-1
  3. Cymbopogon Species; Ethnopharmacology, Phytochemistry and the Pharmacological Importance vol.20, pp.5, 2015, https://doi.org/10.3390/molecules20057438
  4. essential oil in food preservation: Recent advances and future perspectives vol.57, pp.12, 2017, https://doi.org/10.1080/10408398.2015.1016140