Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지
DOI QR코드

DOI QR Code

Genotype Analysis of apoVLDL-II Gene in Korean Chicken Breeds

한국 재래닭의 경제형질 개량을 위한 apoVLDL-II 유전자의 유전자형 분석

  • Jung, K.C. (Division of Animal Science and Resources, Chungnam National University) ;
  • Lee, Y.J. (Division of Animal Science and Resources, Chungnam National University) ;
  • Bhuiyan, M.S.A. (Division of Animal Science and Resources, Chungnam National University) ;
  • Jang, B.K. (National Institute of Animal Science, R.D.A.) ;
  • Choi, K.D. (The Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, Chungnam National University)
  • 정기철 (충남대학교 동물자원과학부) ;
  • 이유주 (충남대학교 동물자원과학부) ;
  • ;
  • 장병귀 (농촌진흥청 난지농업연구소) ;
  • 최강덕 (한경대학교 생물정보통신대학원) ;
  • 이준헌 (충남대학교 동물자원과학부)
  • Published : 2009.01.02
Download PDF
⟨ Previous Next ⟩

Abstract

The very low density apolipoprotein-II (apoVLDL-II) gene is closely related with the constitution of the lipoprotein in various tissues. The apoVLDL-II gene have main functions for reducing fat elements from tissues and muscles. Previous results indicated that the polymorphisms in apoVLDL-II gene were positively related with growth and body composition traits in chicken. In this study, we analyzed previously identified apoVLDL-II gene polymorphisms using the PCR-RFLP method and investigated allele and genotype frequencies in three chicken breeds. Data indicated that Korean native chicken and Korean Oge chicken have similar B and F gene frequencies, indicating that this marker can be used for the improvement of growth and body composition traits in those breeds and can be used as marker assisted selection with further verifications.

초저밀도 아포지단백(Very Low Density Apolipoprotein-II)은 조직의 지방단백질 분비 조성과 밀접한 관련이 있다고 알려진 유전자로서 최근 닭에서 성장 및 체구성과 매우 높은 연관이 있다고 알려져 있다. 본 연구는 닭의 3품종에서 PCR-RFLP 방법을 통해 apoVLDL-II 유전자의 유전자형을 분석하고 Broiler 형(B)과 Fayoumi 형(F)을 결정하였다. 각 품종간 유전자형 빈도는 한국 재래계에서 BB가 0.37, BF가 0.43, FF가 0.2로 검출되었고, 오계에서 BB가 0.2, BF가 0.6, FF가 0.2로 검출되었으며, 백색레그혼에서 BB가 0.16, BF가 0.84로 검출되었다. Broiler 형은 성장이나 체구성을 높이는 방향으로 작용한다는 기존의 보고를 바탕으로 본 연구에서 나타난 집단내 다양한 유전자형 분포는 재검증 작업을 거쳐 육종에 의해 이 형질들을 개량할 수 있음을 의미한다.

Keywords

References

  1. Abasht B, Dekkers JC, Lamont SJ 2006 Review of quantitative trait loci identified in the chicken. Poult Sci 85:2079-2096. https://doi.org/10.1093/ps/85.12.2079
  2. Andersson L 2001 Genetic dissection of phenotypic diversity in farm animals. Nat Rev Genet 2:130-138. https://doi.org/10.1038/35052563
  3. Burt DW 2005 Chicken genome: current status and future opportunities. Genome Res 15:1692-1698. https://doi.org/10.1101/gr.4141805
  4. Douaire M, Le Fur N, El Khadir-Mounier C, Langlois P, Flamant F, Mallard J 1992 Identifying genes involved in the variability of genetic fatness in the growing chicken. Poult Sci 71:1911-1920. https://doi.org/10.3382/ps.0711911
  5. Falconer DS, Mackey TFC 1996 Introduction to quantitative genetics. 4th ed. Addison Wesley Longman Limited, Edinburgh Gate, Harlow.
  6. Hocking PM 2005 Review of QTL results in chicken. World's Poult Sci J 61:215-226. https://doi.org/10.1079/WPS200461
  7. Li H, Deeb N, Zhou H, Ashwell CM, Lamont SJ 2005 Chicken quantitative trait loci for growth and body composition associated with the very low density apolipoprotein-II gene. Poult Sci 84:697-703. https://doi.org/10.1093/ps/84.5.697
  8. Luskey KL, Brown MS, Goldstein JL 1974 Stimulation of the synthesis of very low density lipoproteins in rooster liver by estradiol. J Biol Chem 25:5939-5947.
  9. van het Schip AD, Meijlink FC, Strijker R, Gruber M, van Vliet AJ, van de Klundert JA, Ab G 1983 The nucleotide sequence of the chicken apo very low density lipoprotein II gene. Nucleic Acids Res 11:2529-2540. https://doi.org/10.1093/nar/11.9.2529
  10. Van Laere AS, Nguyen M, Braunschweig M, Nezer C, Collette C, Moreau L, Archibald AL, Haley CS, Buys N, Tally M, Andersson G, Georges M, Andersson L 2003 A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425(6960):832-836. https://doi.org/10.1038/nature02064
  11. Wallis JW, Aerts J, Groenen MA, Crooijmans RP, Layman D, Graves TA, Scheer DE, Kremitzki C, Fedele MJ, Mudd NK, Cardenas M, Higginbotham J, Carter J, McGrane R, Gaige T, Mead K, Walker J, Albracht D, Davito J, Yang SP, Leong S, Chinwalla A, Sekhon M, Wylie K, Dodgson J, Romanov MN, Cheng H, de Jong PJ, Osoegawa K, Nefedov M, Zhang H, McPherson JD, Krzywinski M, Schein J, Hillier L, Mardis ER, Wilson RK, Warren WC 2004 A physical map of the chicken genome. Nature 432(7018):761-764. https://doi.org/10.1038/nature03030
  12. Weinshilboum R, Wang L 2004 Pharmacogenomics: bench to bedside. Nat Rev Drug Discov 9:739-748.
  13. 이준헌 박찬규 2005 주요 가축들의 유용유전자 및 질병관련 유전자 연구 현황. 한국동물자원과학회지 47:325-340. https://doi.org/10.5187/JAST.2005.47.3.325