Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Ultrastructure of Sarcocystis grueneri-like Sarcocysts from Cardiac Muscle of Red Deer (Cervus elaphus) in Korea

한국산 Red Deer (Cervus elaphus)의 심근에서 관찰된 Sarcocystis grueneri 양 포낭의 투과전자현미경 소견

  • Son, Hwa-Young (Research Institute of Veterinary Medicine.College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Nam-Soo (Research Institute of Veterinary Medicine.College of Veterinary Medicine, Chungnam National University) ;
  • Ryu, Si-Yun (Research Institute of Veterinary Medicine.College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Hyeon-Cheol (School of Veterinary Medicine, Kangwon National University) ;
  • Rhee, Ju-Hee (Gyeonggi Veterinary Service Center) ;
  • Cho, Jeong-Gon (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Park, Bae-Keun (Research Institute of Veterinary Medicine.College of Veterinary Medicine, Chungnam National University)
  • 손화영 (충남대학교 수의과대학.동물의과학연구소) ;
  • 김남수 (충남대학교 수의과대학.동물의과학연구소) ;
  • 류시윤 (충남대학교 수의과대학.동물의과학연구소) ;
  • 김현철 (강원대학교 수의과대학) ;
  • 이주희 (경기도 가축위생연구소) ;
  • 조정곤 (전북대학교 수의과대학) ;
  • 박배근 (충남대학교 수의과대학.동물의과학연구소)
  • Published : 2009.12.31
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Abstract

Sarcocystis grueneri-like sarcocysts were found from the cardiac muscles of a rearing red deer (Cervus elaphus) carcass in Korea. In the light microscopical examination of sarcocysts, they were oval to spherical cysts and 90-170$\times$110-380 ${\mu}m$ in size. However, there was no inflammation and myofiber degeneration. In the transmission electron microscope, these cysts were located within the sarcoplasm of the host cell and filled with merozoites. The sarcocysts were enclosed by a very thin wall (0.45-0.6 ${\mu}m$ thick) that consists of protrusions and ground substance. The primary cyst wall formed numerous strip-like protrusions which were 0.2-0.3 ${\mu}m$ wide and up to 4.2 ${\mu}m$ long. The protrusions were running in parallel with the surface of the cyst. A characteristic of the cyst wall was absent of fibrils inside the protrusions. Merozoites in the compartment measured about $15\times4\;{\mu}m$. The merozoite consisted of four regions: micronemes and rhoptries, amylopectin granule, nucleus, and amylopectin granules. The number of rhoptry was counted in 7-13.

한국산 양축 엘크사슴 (Cervus elaphus)의 심장 조직에서 Sarcocystis grueneri 양 포낭을 광학 및 투과전자현미경으로 관찰하였다. 심장조직의 병리소견에서는 다수의 포낭이 관찰되었으며, 염증 및 근섬유의 변성은 관찰되지 않았다. 염증 및 근섬유의 변성은 관찰되지 않았으며 다수의 포낭이 관찰되었다. 포낭은 난형에서 난원형 이었으며, 크기는 90-170$\times$ 110-380 ${\mu}m$로 계측되었다. 투과전자현미경 소견에서 포낭은 숙주세포 유래의 sarcoplasm에 싸여 있었고 0.45-0.6 ${\mu}m$ 두께의 매우 얇은 포낭벽을 갖고 있었으며, 포낭벽은 protrusion 및 ground substance로 구성되어 있었다. 다수의 길고 가느다란 protrusion 층의 높이는 0.2-0.3 ${\mu}m$, protrusion의 길이는 최장 4.2 ${\mu}m$로 계측되었다. Protrusion은 포낭의 표면과 평행하게 주행하였다. 포낭은 중격에 의하여 소실로 나뉘어져 있었고 소실에는 크기가 약 $15\times4\;{\mu}m$인 다수의 merozoites가 존재하였다. 관찰된 모든 포낭은 같은 발육기를 갖고 있었다. Merozoite는 4개 구역(micronemes and rhoptries, amylopectin granules, 핵 및 amylopectin granules)으로 구분되었으며 7-11 개의 rhoptry가 충체의 전방에 분포하였다.

Keywords

References

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