Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Leukocyte Markers Differentiate Non-Infected from Spontaneously Infected Dairy Cows

우유의 체세포내 면역 표지자 분석을 통한 소 유방염 진단

  • Yu, Do-Hyeon (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Jong-Hyeon (College of Veterinary Medicine, Chonbuk National University) ;
  • Song, Ru-Hui (College of Veterinary Medicine, Chonbuk National University) ;
  • Noh, Dong-Ho (College of Veterinary Medicine, Chonbuk National University) ;
  • Li, Ying-Hua (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Mi-Jin (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Jin-Ho (College of Veterinary Medicine, Chonbuk National University)
  • Published : 2009.12.31
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Abstract

Spontaneously infected and non-infected dairy cows were assessed in a cross-sectional study aimed at determining whether bovine leukocyte markers may diagnose intra-mammary infections (bovine mastitis). Animals located in herds where bovine mastitis was highly prevalent were investigated (n = 31 animals). The expression of three cell-surface markers (CD11b, CD4 and CD8) was assessed, and the somatic cell count (SCC) and bacteriological analyses (both cultures and PCR tests) were also conducted. Cows identified as infected revealed statistically significant higher milk leukocyte CD11b, CD4 percentage and milk CD4/CD8 ratios than non-infected cows. Immunological markers may diagnose spontaneous bovine mastitis.

본 연구는 소의 유선 내 감염(유방염)과 체세포 면역 표지자의 상관 관계 분석을 위하여 자연적으로 감염된 젖소와 건강한 젖소의 우유를 비교한 단면조사 연구이다. 유방염에 이환된 31마리의 국내 젖소의 우유에서 세 가지의 체세포 면역 표지자(CD11b, CD4, CD8)의 발현과 체세포 수(SCC), 그리고 세균학적인 분석(배양 및 PCR검사)을 통한 감염 여부 및 병원체의 종류에 대하여 분석하였다. 그 결과 감염된 젖소의 우유는 건강한 젖소의 우유보다 체세포 내의 CD11b와 CD4발현이 유의적으로 증가하였으며, CD4/CD8비율도 높았다. 그러나, 병원체의 종류에 따른 증가된 체세포 면역 표지자와는 커다란 연관성을 보이지 않았으나, 감염된 병원체의 수와 관련해서는, 체세포 면역 표지 인자 CD11b, CD4의 발현 그리고 CD4/8 비율의 증가와 현저한 관련이 있었다. 이러한 연구 결과로 볼 때, 면역 표지자를 이용한 우유의 체세포 분석을 통하여 특발성 소 유방염의 진단 및 관리에 유용하게 활용될 수 있을 것이다.

Keywords

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