Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Detection of Antibodies Reacting with Anaplasma phagocytophilum and Ehrlichia chaffeensis from Cats, Horses and Cattle in Korea

한국에서 사육되는 고양이, 말, 소로부터 Anaplasma phagocytophilum과 Ehrlichia chaffeensis에 대한 항체 검출

  • Chae, Joon-Seok (The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Heo, Eun-Jeong (National Veterinary Research and Quarantine Service) ;
  • Park, Jin-Ho (College of Veterinary Medicine, Chonbuk National University) ;
  • Choi, Kyoung-Seong (Department of Animal Science, Kyungpook National University) ;
  • Dumler, J. Stephen (Division of Medical Microbiology, Department of Pathology, The Johns Hopkins Medical Institutions) ;
  • Lee, Sung-Soo (Jeju Sub-station, National Institute of Animal Science, Rural Development Administration) ;
  • Kang, Tae-Young (Jeju Sub-station, National Institute of Animal Science, Rural Development Administration) ;
  • Yang, Jae-Hyuk (Korea Racing Authority) ;
  • Kim, Do-Young (Korea Racing Authority) ;
  • Kim, Joon-Gyu (Korea Racing Authority) ;
  • Choi, Gui-Cheol (Korea Racing Authority) ;
  • Kang, Mun-Il (College of Veterinary Medicine, Chonnam National University)
  • Published : 2009.12.31
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Abstract

Antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected by the immunofluorescent antibody (IFA) test in sera collected from cats, thoroughbred horses and Holstein cattle in Gwangju, Jeonju and Jeju Island of Korea. Two hundred fifty four sera (33 feral and pet cats, 92 grazing horses and 129 grazing cattle) were obtained from Republic of Korea. Antibodies to A. phagocytophilum (titer $\geq$ 80) were detected in 6 of the 33 feral and pet cats (18.2%), and 1 seropositive cat (3.0%) also had antibodies to E. chaffeensis. Only 1 of 129 (0.8%) cattle and 2 of 92 (2.2%) horses had antibodies to A. phagocytophilum. Antibodies to E. chaffeensis were not detected in either of these animals. This is the first report of serological evidence of A. phagocytophilum and E. chaffeensis from cats, cattle and horses in Korea. These rickettsial agents could have an important impact on human health or impact animal health with economic losses among industrial grazing animals in Korea.

국내 전주, 광주 그리고 제주에서 고양이, 더러브렛 말, 홀스테인 소로부터 진드기 매개성 인수공통 병원체인 Anaplasma phagocytophilum과 Ehrlichia chaffeensis에 대한 항체가 조사를 위하여 면역형광항체법을 이용하였다. 본 연구를 위하여 254마리의 혈청(33마리의 애완 및 길거리 고양이, 92마리의 방목장 말 그리고 129마리의 방목장소)을 수집하였다. 33마리의 고양이 중에서 6마리가 A. phagocytophilum(titer $\geq$ 80)에 대한 양성항체가 검출되어 18.2%의 양성율을 나타내었으며, 1마리에 있어서는 E. chaffeensis에 대한 항체가 검출되어 3%의 양성율을 나타내었다. 소에 있어서는 129마리 중에서 1마리에서 그리고 말에 있어서는 92마리 중에서 2마리에서 A. phagocytophilum에 대한 양성항체가 검출되어 각각 0.8%와 2.2%의 양성율을 나타내었으나 E. chaffeensis에 대한 항체는 모두 음성 결과를 나타내었다. 이 결과는 국내 사육 고양이, 말 그리고 소에 있어서 A. phagocytophilum과 E. chaffeensis에 대한 혈청학적 양성결과로서 국내에서 처음 보고되는 자연감염 예이며, 진드기 서식지역의 방목동물에서는 이들 질병의 감염으로 경제성 손실이 우려되며 예방대책이 마련되어야 할 것으로 판단된다.

Keywords

References

  1. Anderson BE, Sims KG, Olson JG, Chiles JE, Piesman JE, Happ CM, Maupin GO, Johnson BJB. Amblyomma americanum: a potential vector of human ehrlichiosis. Am J Trop Med Hyg 1993; 49: 239-244
  2. Asanovich KM, Bakken JS, Madigan JE, Aguero-Rosenfeld M, Wormser GP, Dumler JS. Antigenic diversity of granulocytic Ehrlichia isolates from humans in Wisconsin and New York and a horse in California. J Infect Dis 1997; 176: 1029-1034 https://doi.org/10.1086/516529
  3. Bakken JS, Dumler JS, Chen SM, Eckman MR, Van Etta LL, Walker DH, Human granulocytic ehrlichiosis in the upper Midsest United States. A new species emerging? JAMA 1994; 272: 212-218 https://doi.org/10.1001/jama.272.3.212
  4. Bakken JS, Krueth J, Wilson-Nordskog C, Tilden RL, Asanovich K, Dumler JS. Clinical and laboratory characteristics of human granulocytic ehrlichiosis. JAMA 1996; 275:199-205 https://doi.org/10.1001/jama.275.3.199
  5. Baumgarten BU, Rollinghoff M, Bogdan C. Prevalence of Borrelia burgdorferi and granulocytic and monocytic Ehrlichiae in Ixodes ricinus ticks from southern Germany. J Clin Microbiol 1999; 37: 3448-3451
  6. Breitschwerdt EB, Hegarty BC, Hancock SI. Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii. J Clin Micribiol 1998; 36: 2645-2651
  7. Brouqui P, Dumler JS. Serologic evidence of human monocytic and granulocytic ehrlichiosis in Israel. Emerg Infect Dis 2000; 6: 314-315 https://doi.org/10.3201/eid0603.000316
  8. Chae JS, Kim CM, Kim EH, Hur EJ, Klein TA, Kang TK, Lee HC, Song JW. Molecular epidemiological study for tickborne disease (Ehrlichia and Anaplasma spp.) surveillance from wild rodents and mustelids at selected U.S. military training sites/installations in Korea. Ann N Y Acad Sci 2003; 990: 118-125 https://doi.org/10.1111/j.1749-6632.2003.tb07349.x
  9. Chae JS, Yu do H, Shringi S, Klein TA, Kim HC, Chong ST, Lee IY, Foley J. Microbial pathogens in ticks, rodents and a shrew in northern Gyeonggi-do near the DMZ, Korea. J Vet Sci. 2008; 9: 285-293 https://doi.org/10.4142/jvs.2008.9.3.285
  10. Cao WC, Zhao QM, Zhang PH, Dumler JS, Zhang XT, Fang LQ, Yang H. Granulocytic ehrlichiae in Ixodes persulcatus ticks from an area in China where Lyme disease is endemic. J Clin Microbiol 2000; 38: 4208-4210
  11. Comer JA, Nicholson WL, Olson JG, Childs JE. Serologic testing for human granulocytic ehrlichiosis at a national referral center. J Clin Microbiol 1999; 37: 558-564
  12. Dumler JS, Asanovich KM, Bakken JS, Richter P, Kimsey R, Madigan JE. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic ehrlichia. J Clin Micribiol 1995; 33: 1098-1103
  13. Engvall EO, Pettersson B, Persson M, Artursson K, Johansson KE. A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses, and cattle. 1996; 34: 2170-2174
  14. Gordon WS, Broenlee A, Wilson DR, MacLeod J, "Tick-borne fever." (A hitherto undescribed disease of sheep). J Comp Pathol Therap 1932; 65: 301-307
  15. Heo EJ, Park JH, Koo JR, Park MS, Park MY, Dumler JS, Chae JS. Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients. J Clin Microbial 2002;40: 3082-3085 https://doi.org/10.1128/JCM.40.8.3082-3085.2002
  16. Inokuma H, Nane G, Uechi T, Yonahara Y, Brouqui P, Okuda M, Onishi T. Survey of tick infestation and tickborne ehrlichial infection of dogs in Ishigaki Island, Japan. J Vet Med Sci 2001; 63: 1225-1227 https://doi.org/10.1292/jvms.63.1225
  17. Kim CM, Kim MS, Park MS, Park JH, Chae JS. Identification of Ehrlichia chaffeensis, Anaplasma phagocytophila, and A. bovis in Haemaphysalis longicornis and Ixodes persulcatus ticks from Korea. Vector Borne Zoonotic Dis. 2003; 3: 17-26 https://doi.org/10.1089/153036603765627424
  18. Kim CM, Yi YH, Yu DH, Lee MJ, Cho MR, Desai AR, Shringi S, Klein TA, Kim HC, Song JW, Baek LJ, Chong ST, O’Guinn ML, Lee SS, Lee IY, Park JH, Foley J, Chae JS. Tick-borne rickettsial pathogens in ticks and small mammals in Korea. Appl Environ Microbiol 2006; 72: 5766-5776 https://doi.org/10.1128/AEM.00431-06
  19. Lee SO, Na DK, Kim CM, Li YH, Cho YH, Park JH, Lee JH, Eo SK, Klein TA, Chae JS. Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene. J Vet Sci 2005; 6: 151-155
  20. Lockhart JM, Davidson WR, Stallknecht DE, Dawson JE, Howerth EW, Isolation of Ehrlichia chaffeensis from wild white-tailed deer (Odocoileus virginianus) confirms their role as natural reservoir hosts. J Clin Microbiol 1997; 35: 1681-1686
  21. Massung RF, Slater K, Owens JH, Nicholson WL, Mather TN, Solberg VB, Olson JG. Nested PCR assay for detection of granulocytic ehrlichiae. J Clin Microbiol 1998; 36: 1090-1095
  22. Neer TM. Ehrlichiosis. Canine monocytic and granulocytic ehrlichiosis. In: Greene CE. (Ed.). Infectious disease of the dog and cat. Philadelphia: Saunders. 1998: 139-154
  23. Pancholi P, Kolbert CP, Mitchell PD, Reed KD, Dumler JS, Bakken JS, Telford III SR, Persing DH. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J Infect Dis 1995; 172: 1007-1012 https://doi.org/10.1093/infdis/172.4.1007
  24. Park JH, Heo EJ, Choi KS, Dumler JS, Chae JS. Detection of antibodies to Anaplasma phagocytophila and Ehrlichia chaffeensis antigens in sera of Korean patients by Western immunoblotting and indirect immunofluorescence assay. Clin Diag Lab Immunol 2003; 10: 1059-1064
  25. Park HS, Lee JH, Jeong EJ, Park TK, Kim TY, Chae JS, Park JH, Klein TA, Jang WJ, Park KH, Lee SH. Differentiation of Anaplasmataceae through partial groEL gene analysis. Microbiol Immunol 2005; 49: 655-662
  26. Parola P, Raoult D. Ticks and tick-borne bacterial diseases in humans: an emerging infectious threat. Clin Infect Dis 2001; 32: 897-928 https://doi.org/10.1086/319347
  27. Rikihisa Y. New findings on members of the family Anaplasmataceae of veterinary importance. Ann N Y Acad Sci 2006; 1078: 438-445 https://doi.org/10.1196/annals.1374.083
  28. Rikihisa Y. The tribe Ehrlichieae and ehrlichial diseases. Clin Microbial Rev 1991; 4: 286-308
  29. Unver A, Felek S, Paddock CD, Zhi N, Horowitz HW, Wormser GP, Cullman LC, Rikihisa Y. Western blot analysis of sera reactive to human monocytic ehlrichiosis and human granulocytic ehrlichiosis agents. J Clin Microbiol 2001; 39: 3982-3986 https://doi.org/10.1128/JCM.39.11.3982-3986.2001
  30. Walls JJ, Aguero-Rosenfeld M, Bakken JS, Goodman JL, Hossain D, Johnson RC, Dumler JS. Inter- and intralaboratory comparison of Ehrlichia equi and human granulocytic ehrlichiosis (HGE) agent strains for serodiagnosis of HGE by the immunofluorescent-antibody test. J Clin Microbiol 1999; 37: 2968-2973
  31. Waner T, Strenger C, Keysary A, Harrus S. Kinetics of serologic cross-reactions between Ehrlichia canis and the Ehrlichia phagocytophila genogroups in experimental E. canis infection in dogs. Vet Immunol Immunopathol 1998; 66: 237-243 https://doi.org/10.1016/S0165-2427(98)00198-6
  32. Wong SJ, Brady GS, Dumler JS. Serological responses to Ehrlichia equi, Ehrlichia chaffeensis, and Borrelia burgdorferi in patients from New York State. J Clin Microbiol 1997; 35: 2198-2205
  33. Yu DH, Li YH, Yoon JS, Lee JH, Lee MJ, Yu IJ, Chae JS, Park JH. Ehrlichia chaffeensis infection in dogs in South Korea.Vector Borne Zoonotic Dis. 2008; 8: 355-358 https://doi.org/10.1089/vbz.2007.0226
  34. Zhi N, Ohashi N, Rikihisa Y, Horowitz HW, Wormser GP, Hechemy K. Cloning and expression of the 44-kilodalton major outer membrane protein gene of the human granulocytic ehrlichiosis agent and application of the recombinant protein to serodiagnosis. J Clin Microbiol 1998; 36: 1666-1673