International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Assessment of Genetic Diversity in Different Populations of Raily Ecorace of Indian Tasar Silkworm, Antheraea Mylitta Drury Using ISSR Markers

  • Srivastava, Ashok Kumar (Silkworm Breeding and Genetics Division, Central Tasar Research and Training Institute, Central Silk Board) ;
  • Kar, Prasanta Kumar (Silkworm Breeding and Genetics Division, Central Tasar Research and Training Institute, Central Silk Board) ;
  • Sinha, Ravibhushan (Silkworm Breeding and Genetics Division, Central Tasar Research and Training Institute, Central Silk Board) ;
  • Sinha, Manoj Kumar (Silkworm Breeding and Genetics Division, Central Tasar Research and Training Institute, Central Silk Board) ;
  • Vijayaprakash, Nanjappa Basavappa (Silkworm Breeding and Genetics Division, Central Tasar Research and Training Institute, Central Silk Board)
  • Published : 2009.12.31
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Abstract

Raily ecorace of Indian tasar silkworm is wild in nature and distributed abundantly in dense deciduous forest on Shorea robusta (Sal) in Bastar ($17^{\circ}4'$ and $20^{\circ}34'$ N, $80^{\circ}15'$ and $82^{\circ}15'$ E and altitude ranging from 150 to 1200 mMSL) forest ranges of Chhattisgarh, India. It is represented by about 20 populations. Out of those, eleven populations showed intra- as well as inter- population variability based on phenotypic expression and also in major economic traits viz. cocoon weight, shell weight, filament length and denier. Genetic diversity in these eleven populations was studied using Inter-Simple Sequence Repeat (ISSR) markers. The band profiles generated with eight ISSR primers have depicted variation in band size. All the primers exhibited polymorphism which is an indicative of the genetic variation in individual Raily silkworm. Among the populations, total polymorphism recorded was 76%. The population genetic aspects assessed through POPGENE software package are discussed in the paper. Nei's gene diversity (h) ranged from 0.194 to 0.337 exhibiting high heterozygosity. Relevance of the present study is of high significance in formulating conservation strategies and sustainable utilization of the economically important Raily ecorace of Antheraea mylitta.

Keywords

References

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