The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of a Genetic Locus Related to Antivirus Production in Pseudomonas fluorescence strain Gpf01 Against Cucumber mosaic virus

  • Cho, Sae-Youll (Department of Bio-resource of Technology, Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lee, Seon-Hwa (Department of Bio-resource of Technology, Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Park, Su-Jin (Life Science Institute, University of Michigan) ;
  • Choi, Kyu-Up (Department of Bio-resource of Technology, Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Cho, Jun-Mo (Department of Bio-resource of Technology, Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Hur, Jang-Hyun (Division of Biological Environment, College of Agriculture and Life Science, Kangwon National University) ;
  • Shrestha, Anupama (Department of Bio-resource of Technology, Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lim, Chun-Keun (Department of Bio-resource of Technology, Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University)
  • Published : 2009.03.31
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Abstract

Pseudomonas fluorescens strain Gpf01, isolated from ginseng rhizosphere showed antiviral activity against Cucumber mosaic virus, when tested in a local host of CMV, Chenopodium amaranticolor. Transposon mutant library of Gpf01 was prepared using pGS9::Tn5 and the mutant Gpf01-RS19 was found to loose antiviral production. We developed primers from the flanking region of Tn5 and found a cosmid clone pAV1123, harboring 1.2 kb antiviral compound producing (avcf01) locus. When a sub-clone pPH9, which carried 9.3 kb region of pAV1123, was introduced into antivirus deficient P. fluorescens wild type strain B16, it exhibited antiviral activity. Using Tn3-gus mutagenesis and complementation analysis, it was found that the genes related to antiviral activity production resided in a 9.3 kb HindIII-HindIII fragment of pAV1123, indicating that the plasmid carries an essential genes promoting antiviral activity.

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References

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