구강회복응용과학지 (Journal of Dental Rehabilitation and Applied Science)

  • 제25권3호
  • /
  • Pages.293-306
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  • 2009
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  • 2384-4353(pISSN)
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  • 2384-4272(eISSN)
대한턱관절교합학회 (Korean Academy of Stomatognathic Function and Occlusion)
권호 표지

티타니움 표면의 알칼리-열처리가 골모세포의 분화에 미치는 영향

The Effect of Alkali- and Heat-Treated Titanium Surfaces on Differentiation of Osteoblast

  • 강충희 (전남대학교 치의학전문대학원 보철학교실) ;
  • 방몽숙 (전남대학교 치의학전문대학원 보철학교실) ;
  • 양홍서 (전남대학교 치의학전문대학원 보철학교실) ;
  • 박상원 (전남대학교 치의학전문대학원 보철학교실) ;
  • 임현필 (전남대학교 치의학전문대학원 보철학교실)
  • Kang, Choong Hee (Department of Prosthodontics, Division of Dentistry, School of Dentistry, Chonnam National University) ;
  • Vang, Mong-Sook (Department of Prosthodontics, Division of Dentistry, School of Dentistry, Chonnam National University) ;
  • Yang, Hong-so (Department of Prosthodontics, Division of Dentistry, School of Dentistry, Chonnam National University) ;
  • Park, Sang-Won (Department of Prosthodontics, Division of Dentistry, School of Dentistry, Chonnam National University) ;
  • Lim, Hyun-Pil (Department of Prosthodontics, Division of Dentistry, School of Dentistry, Chonnam National University)
  • 투고 : 2009.05.15
  • 심사 : 2009.09.25
  • 발행 : 2009.09.30
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초록

본 연구에서는 알카리 열처리 티타늄 임플란트상에서 백서 태자 두개관 세포의 생물학적 반응을 알아보고자 하여 다음과 같은 결과를 얻었다. 알카리 열처리 티타니움 표면에서 Ti-6Al-4V 합금 표면에서보다 세포증식도가 유의하게 높았다 (p<0.01). 면역효소 분석에서 interleukin $(IL)-1{\beta}$와 IL-6의 합성은 배양 시간이 길수록 증가하였다. Herbimycin으로 전처치한 경우에는 세포 배양 7일 째에 IL-6의 합성이 유의하게 억제되었다(p<0.01). 알카리인산 분해 효소 활성은 알카리 열처리 임플란트에서 타이타늄, Ti-6Al-4V 합금 표면에서 보다 유의하게 더 높았다 (p<0.001). 유전자 분석에서 알카리 열처리 임플란트에서 배양된 세포들의 alkaline phosphatase, bone sialoprotein, receptor activated nuclear factor ligand 유전자의 발현이 증가하였다. Herbimycin과 SB203580으로 전처치시 $IL-1{\beta}-induced$ IL-6 유전자의 발현이 감소하였다. 이상의 결과는 알카리 열처리된 티타늄 임플란트가 조골세포의 분화를 자극하고 골개조를 촉진시킴을 시사한다.

In this study, the biological response of fetal rat calvarial cells on alkali- and heat-treated titanium was assessed. The results were as follows; Cell proliferation on alkali- and heat-treated surfaces showed significantly higher level than on the titanium-6aluminum-4vanadium (weight percentage: 6 % aluminum, 4 % vanadium, Ti-6Al-4V) surface (p<0.01). In ELISA analysis, concentration of $IL-1{\beta}$ and IL-6 were raised when the cells were grown to day 7. Pre-treatment with herbimycin, a known tyrosine kinase inhibitor, suppressed the production of IL-6 (p<0.01). In comparison to commercially pure titanium (grade II, cp-Ti) and Ti-6Al-4V alloy, alkali- and heat-treated titanium enhanced alkaline phosphatase activity (p<0.001). In RT-PCR analysis, alkaline phosphatase, bone sialoprotein, receptor activated nuclear factor ligand mRNA expression was increased alkali- and heat-treated titanium. Herbimycin and SB203580, p38 MAPK inhibitor, were repressed of $IL-1{\beta}-induced$ IL-6 mRNA expression. These results suggest that alkali- and heat-treated titanium stimulate osteoblasts differentiation and facilitate bone remodeling.

키워드

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