Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Pathogen Inducible Voltage-Dependent Anion Channel (AtVDAC) Isoforms Are Localized to Mitochondria Membrane in Arabidopsis

  • Lee, Sang Min (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Hoang, My Hanh Thi (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Han, Hay Ju (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Kim, Ho Soo (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Kyunghee (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Kim, Kyung Eun (Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Kim, Doh Hoon (Faculty of Plant Biotechnology, Dona-A University) ;
  • Lee, Sang Yeol (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Chung, Woo Sik (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
  • Received : 2008.10.06
  • Accepted : 2008.12.18
  • Published : 2009.03.31
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Abstract

Voltage-dependent anion channels (VDACs) are reported to be porin-type, ${\beta}$-barrel diffusion pores. They are prominently localized in the outer mitochondrial membrane and are involved in metabolite exchange between the organelle and the cytosol. In this study, we have investigated a family of VDAC isoforms in Arabidopsis thaliana (AtVDAC). We have shown that the heterologous expression of AtVDAC proteins can functionally complement a yeast mutant lacking the endogenous mitochondrial VDAC gene. AtVDACs tagged with GFP were localized to mitochondria in both yeast and plant cells. We also looked at the response of AtVDACs to biotic and abiotic stresses and found that four AtVDAC transcripts were rapidly up-regulated in response to a bacterial pathogen.

Keywords

Acknowledgement

Supported by : Environmental Biotechnology National Core Research Center, Ministry of Education, Science and Technology, Rural Development Administration, Ministry of Education, Science and Technology in Korea

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