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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Flavonoids inhibit the AU-rich element binding of HuC

  • Kwak, Ho-Joong (Departments of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University) ;
  • Jeong, Kyung-Chae (Molecular Oncology Branch, Division of Basic Sciences, Research Institute, National Cancer Center) ;
  • Chae, Min-Ju (Departments of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University) ;
  • Kim, Soo-Youl (Molecular Oncology Branch, Division of Basic Sciences, Research Institute, National Cancer Center) ;
  • Park, Woong-Yang (Departments of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University)
  • Published : 2009.01.31
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Abstract

Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their $IC_{50}$ values were low ($0.2-1.8\;{\mu}M$).

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References

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