Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
권호 표지

Improvement of the Inferior Epigastric Artery Flap Viability Using Adenovirus-mediated VEGF and COMP-angiopoietin-1

아래쪽배벽동맥피판의 생존향상을 위한 VEGF와 COMP-angiopoietin-1 유전자 치료

  • Yoo, Eun Kyung (Department of Plastic and Reconstructive Surgery Keimyung University School of Medicine) ;
  • Son, Daegu (Department of Plastic and Reconstructive Surgery Keimyung University School of Medicine) ;
  • Kim, Hyung Tae (Department of General Surgery Keimyung University School of Medicine) ;
  • Lee, In Kyu (Department of Internal Medicine, Kyungpook National University School of Medicine) ;
  • Choi, Taehyun (Department of Plastic and Reconstructive Surgery Keimyung University School of Medicine) ;
  • Kim, Junhyung (Department of Plastic and Reconstructive Surgery Keimyung University School of Medicine) ;
  • Han, Kihwan (Department of Plastic and Reconstructive Surgery Keimyung University School of Medicine)
  • 유은경 (계명대학교 의과대학 성형외과학교실) ;
  • 손대구 (계명대학교 의과대학 성형외과학교실) ;
  • 김형태 (계명대학교 의과대학 외과학교실) ;
  • 이인규 (경북대학교 의과대학 내분비내과학교실) ;
  • 최태현 (계명대학교 의과대학 성형외과학교실) ;
  • 김준형 (계명대학교 의과대학 성형외과학교실) ;
  • 한기환 (계명대학교 의과대학 성형외과학교실)
  • Received : 2008.08.04
  • Accepted : 2008.10.17
  • Published : 2009.01.15
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Abstract

Purpose: Partial necrosis of skin flaps remains a substantial problem in reconstructive surgery. We investigated the potential use of an adenovirus vector encoding the VEGF, COMP-angiopoietin-1 gene in an attempt to promote the viability of the inferior epigastric artery flap in a rat model. Methods: Three by six cm lower abdominal transverse skin flaps, supplied only by the left inferior epigastric artery, were designed. After skin flap elevation, the adenovirus VEGF and adenovirus COMP-angiopoietin-1 were injected into the distal portion of the flap, which has a high tendency of developing flap ischemia. Control animals were injected with the same volume of normal saline. On 3, 7 and 14 days after the flap elevation, the flap survival and vascularization were assessed using Visitrak digital$^{(R)}$, CD31 immunohistochemistry in addition to evaluating the general histological characteristics. Results: There was a significant increase in the mean percentage of flap viability by 89.8%, 91.1% and 94.8% in flaps transfected with adenovirus VEGF, COMP-angiopoietin-1, coadministraion of VEGF and COMP-angiopoietin-1 at seven days, and by 95.6%, 94.8% and 96.3% at 14 days. Histological assessment revealed that there were more blood vessels formed after adenovirus with VEGF, COMP-angiopoietin-1 or VEGF plus COMP-angiopoietin-1 than with adenovirus Lac Z. Conclusion: The results of this study suggest that adenovirus-mediated VEGF, COMP-angiopoietin-1 gene therapy, promote therapeutic angiogenesis in patients that undergo reconstructive procedures.

Keywords

References

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